E3RC: A step-by-step computational protocol for exploring enhancer RNA expression and regulation using conventional RNA-seq data

E3RC: A step-by-step computational protocol for exploring enhancer RNA expression and regulation using conventional RNA-seq data

Summary: Here, we present a computational framework, E3RC, for exploring enhancer RNA (eRNA) expression and regulation using conventional RNA sequencing (RNA-seq) data. Using a public dataset as an example, we describe step-by-step guidelines for identifying eRNAs and characterizing their expression...

Full description

Saved in:
Bibliographic Details
Main Authors: Hua Yu, Yunjie Liu, Zhouming Zhang, Guanglei Xie, Jingyu Liu, Yijia Liu, Siyue He, Zhiwei Peng, Lu Lu, Fei Li, Xi Wang
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
RNAseq
Sequence analysis
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003958
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary: Here, we present a computational framework, E3RC, for exploring enhancer RNA (eRNA) expression and regulation using conventional RNA sequencing (RNA-seq) data. Using a public dataset as an example, we describe step-by-step guidelines for identifying eRNAs and characterizing their expressional changes and transcriptional regulation across multiple developmental stages of mouse preimplantation embryos.For complete details on the use and execution of this protocol, please refer to Yu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

Similar Items