Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)
To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any qua...
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KeAi Communications Co., Ltd.
2018-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S209531191861963X |
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| author | Wen-wen LIU Min XIN Meng-ji CAO Meng QIN Hui LIU Shou-qi ZHAO Xi-feng WANG |
| author_facet | Wen-wen LIU Min XIN Meng-ji CAO Meng QIN Hui LIU Shou-qi ZHAO Xi-feng WANG |
| author_sort | Wen-wen LIU |
| collection | DOAJ |
| description | To identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA (dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus (HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5' untranslated region (UTR), 3' UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame (ORF) that encodes a deduced 4 867 amino acids (aa) polyprotein with three domains: RdRP, Hel and UGT (UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization (FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower. |
| format | Article |
| id | doaj-art-bfa21f7b01c5453aae7d7e75b9dac7f8 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-bfa21f7b01c5453aae7d7e75b9dac7f82025-08-02T18:23:11ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-10-01171022812291Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.)Wen-wen LIU0Min XIN1Meng-ji CAO2Meng QIN3Hui LIU4Shou-qi ZHAO5Xi-feng WANG6State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. ChinaNational Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, P. R. ChinaNational Agricultural Technology Extension and Service Center, Beijing 100125, P. R. ChinaNational Agricultural Technology Extension and Service Center, Beijing 100125, P. R. ChinaNational Agricultural Technology Extension and Service Center, Beijing 100125, P. R. ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China; Correspondence WANG Xi-fengTo identify the possible quarantine viruses in seven common sunflower varieties imported from the United States of America and the Netherlands, we tested total RNAs extracted from the leaf tissues using next-generation sequencing of small RNAs. After analysis of small RNA sequencing data, no any quarantine virus was found, but a double-stranded RNA (dsRNA) molecule showing typical genomic features of endornavirus was detected in two varieties, X3939 and SH1108. Full-length sequence and phylogenetic analysis showed that it is a novel endornavirus, temporarily named as Helianthus annuus alphaendornavirus (HaEV). Its full genome corresponds to a 14 662-bp dsRNA segment, including a 21-nt 5' untranslated region (UTR), 3' UTR ending with the unique sequence CCCCCCCC and lacking a poly(A) tail. An open reading frame (ORF) that encodes a deduced 4 867 amino acids (aa) polyprotein with three domains: RdRP, Hel and UGT (UDP-glycosyltransferase). HaEV mainly distributed in the cytoplasm but less in the nucleus of leaf cells by fluorescence in situ hybridization (FISH) experiment. This virus has a high seed infection rate in the five varieties, X3907, X3939, A231, SH1108 and SR1320. To our knowledge, this is the first report about the virus of the family Endornaviridae in the common sunflower.http://www.sciencedirect.com/science/article/pii/S209531191861963Xcommon sunflower (Helianthus annuus)next-generation sequencing (NGS)double-stranded RNAEndornaviridae |
| spellingShingle | Wen-wen LIU Min XIN Meng-ji CAO Meng QIN Hui LIU Shou-qi ZHAO Xi-feng WANG Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.) Journal of Integrative Agriculture common sunflower (Helianthus annuus) next-generation sequencing (NGS) double-stranded RNA Endornaviridae |
| title | Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.) |
| title_full | Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.) |
| title_fullStr | Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.) |
| title_full_unstemmed | Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.) |
| title_short | Identification, characterization and full-length sequence analysis of a novel endornavirus in common sunflower (Helianthus annuus L.) |
| title_sort | identification characterization and full length sequence analysis of a novel endornavirus in common sunflower helianthus annuus l |
| topic | common sunflower (Helianthus annuus) next-generation sequencing (NGS) double-stranded RNA Endornaviridae |
| url | http://www.sciencedirect.com/science/article/pii/S209531191861963X |
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