Antitumor Enzyme L-Lyzine-α-Oxidase from Trichoderma harzianum Rifai F-180 and Investigation of Its Action on L-Lysine Oxidation by Capillar Electrophoresis
Trichoderma harzianum Rifai F-180, an organism producing the antitumor enzyme L-lysine-α-oxidase was cultivated and the enzyme was isolated and purified under the manufacturing conditions. The effect of L-lysine-α-oxidase on oxidation of L-lysine was investigated for the first time by capillary elec...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
LLC "Publishing House OKI"
2020-05-01
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| Series: | Антибиотики и Химиотерапия |
| Subjects: | |
| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/537 |
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| Summary: | Trichoderma harzianum Rifai F-180, an organism producing the antitumor enzyme L-lysine-α-oxidase was cultivated and the enzyme was isolated and purified under the manufacturing conditions. The effect of L-lysine-α-oxidase on oxidation of L-lysine was investigated for the first time by capillary electrophoresis and the procedure conditions were developed. The reaction of L-lysine oxidative deamination is described and location of the reaction components picks on the elecrophoregrams was identified. The average rate of the catalytic reaction of L-lysine oxidation equal to 0.46 RU/min (7.7х10-3 RU/sec) was determined. The use of the antitumor enzyme L-lysine-α-oxidase is recommended as a drug for the treatment of superficial tumors and tissue relative oxygen excess. |
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| ISSN: | 0235-2990 |