Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize (Zea mays L.)
Postemergence application of nicosulfuron for weed control in maize fields can cause great damage to certain maize inbred lines and hybrids. Two maize genotypes, tolerant inbred (HBR) and sensitive inbred (HBS), were found to significantly differ in their phenotypic responses to nicosulfuron, with t...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2018-08-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311918619215 |
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| author | Xiao-min LIU Xian XU Bing-hua LI Xiao-xia YAO Huan-huan ZHANG Gui-qi WANG Yu-jun HAN |
| author_facet | Xiao-min LIU Xian XU Bing-hua LI Xiao-xia YAO Huan-huan ZHANG Gui-qi WANG Yu-jun HAN |
| author_sort | Xiao-min LIU |
| collection | DOAJ |
| description | Postemergence application of nicosulfuron for weed control in maize fields can cause great damage to certain maize inbred lines and hybrids. Two maize genotypes, tolerant inbred (HBR) and sensitive inbred (HBS), were found to significantly differ in their phenotypic responses to nicosulfuron, with the EC50 (50% effective concentration) values differed statistically (763.6 and 5.9 g a.i. ha–1, respectively). Pre-treatment with malathion, a known cytochrome P450 inhibitor, increased nicosulfuron injury in both HBR and HBS. Our results support the hypothesis that nicosulfuron selectivity in maize is associated with cytochrome P450 metabolism. Further analysis of the maize genome resulted in the identification of 314 full length cytochrome P450 monooxygenase (CYP) genes. These genes were classified into 2 types, 10 clans and 44 families. The CYP71 clan was represented by all A-type genes (168) belonging to 17 families. Nine clans possessed 27 families containing 146 non-A-type genes. The consensus sequences of the heme-binding regions of A-type and non-A-type CYP proteins are ‘PFGXGRRXCPG’ and ‘FXXGPRXCXG’, respectively. Illumina transcriptome sequence results showed that there were 53 differentially expressed CYP genes on the basis of high variation in expression between HBS and HBR, nicosulfuron-treated and untreated samples. These genes may contribute to nicosulfuron tolerance in maize. A hierarchical clustering analysis obtained four main clusters named C1 to C4 in which 4, 15, 21, and 13 CYP genes were found in each respective cluster. The expression patterns of some CYP genes were confirmed by RT-qPCR analysis. The research will improve our understanding of the function of maize cytochrome P450 in herbicide metabolism. |
| format | Article |
| id | doaj-art-be5195c7c58746b999bf0fd064202ccd |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-be5195c7c58746b999bf0fd064202ccd2025-08-02T18:15:09ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-08-0117817901799Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize (Zea mays L.)Xiao-min LIU0Xian XU1Bing-hua LI2Xiao-xia YAO3Huan-huan ZHANG4Gui-qi WANG5Yu-jun HAN6Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, P.R.China; LIU Xiao-min, Tel: +86-311-87670616Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, P.R.ChinaInstitute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, P.R.ChinaXingtai Academy of Agriculture Sciences, Xingtai 054000, P.R.ChinaInstitute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, P.R.ChinaInstitute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, P.R.China; Correspondence WANG Gui-qi, Tel: +86-311-87670632College of Agriculture, Northeast Agricultural University, Harbin 150030, P.R.China; HAN Yu-jun, Tel: +86-451-55190447Postemergence application of nicosulfuron for weed control in maize fields can cause great damage to certain maize inbred lines and hybrids. Two maize genotypes, tolerant inbred (HBR) and sensitive inbred (HBS), were found to significantly differ in their phenotypic responses to nicosulfuron, with the EC50 (50% effective concentration) values differed statistically (763.6 and 5.9 g a.i. ha–1, respectively). Pre-treatment with malathion, a known cytochrome P450 inhibitor, increased nicosulfuron injury in both HBR and HBS. Our results support the hypothesis that nicosulfuron selectivity in maize is associated with cytochrome P450 metabolism. Further analysis of the maize genome resulted in the identification of 314 full length cytochrome P450 monooxygenase (CYP) genes. These genes were classified into 2 types, 10 clans and 44 families. The CYP71 clan was represented by all A-type genes (168) belonging to 17 families. Nine clans possessed 27 families containing 146 non-A-type genes. The consensus sequences of the heme-binding regions of A-type and non-A-type CYP proteins are ‘PFGXGRRXCPG’ and ‘FXXGPRXCXG’, respectively. Illumina transcriptome sequence results showed that there were 53 differentially expressed CYP genes on the basis of high variation in expression between HBS and HBR, nicosulfuron-treated and untreated samples. These genes may contribute to nicosulfuron tolerance in maize. A hierarchical clustering analysis obtained four main clusters named C1 to C4 in which 4, 15, 21, and 13 CYP genes were found in each respective cluster. The expression patterns of some CYP genes were confirmed by RT-qPCR analysis. The research will improve our understanding of the function of maize cytochrome P450 in herbicide metabolism.http://www.sciencedirect.com/science/article/pii/S2095311918619215cytochrome P450Zea mays L.malathionnicosulfuronherbicide metabolism |
| spellingShingle | Xiao-min LIU Xian XU Bing-hua LI Xiao-xia YAO Huan-huan ZHANG Gui-qi WANG Yu-jun HAN Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize (Zea mays L.) Journal of Integrative Agriculture cytochrome P450 Zea mays L. malathion nicosulfuron herbicide metabolism |
| title | Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize (Zea mays L.) |
| title_full | Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize (Zea mays L.) |
| title_fullStr | Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize (Zea mays L.) |
| title_full_unstemmed | Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize (Zea mays L.) |
| title_short | Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize (Zea mays L.) |
| title_sort | genomic and transcriptomic insights into cytochrome p450 monooxygenase genes involved in nicosulfuron tolerance in maize zea mays l |
| topic | cytochrome P450 Zea mays L. malathion nicosulfuron herbicide metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2095311918619215 |
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