10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus
Antimicrobial resistance (AMR) is a global concern that undermines microbial disease treatment and prevention. WHO and World Bank's EcoAMR report predicts that AMR could cause 39 million deaths and $3.4 trillion in annual GDP losses by the year 2050. This is particularly critical with S. aureus...
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Elsevier
2025-12-01
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| Series: | Biofilm |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207525000553 |
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| author | Hiren Dodia Suvendu Ojha Puja Chatterjee Tushar Kant Beuria |
| author_facet | Hiren Dodia Suvendu Ojha Puja Chatterjee Tushar Kant Beuria |
| author_sort | Hiren Dodia |
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| description | Antimicrobial resistance (AMR) is a global concern that undermines microbial disease treatment and prevention. WHO and World Bank's EcoAMR report predicts that AMR could cause 39 million deaths and $3.4 trillion in annual GDP losses by the year 2050. This is particularly critical with S. aureus, a cause of diverse infections like skin abscesses and pneumonia, where antibiotic resistance increases mortality and hinders treatment. Biofilms are one of the major causes of multi-drug resistance in S. aureus, and their inhibition can restore antibiotic sensitivity. In this study, through screening of the LOPAC drug library, we identified several compounds that exhibit biofilm inhibitory properties against multi-drug-resistant S. aureus without affecting its growth. The compound 10058-F4 was found to have the strongest anti-biofilm activity (>70 % inhibition) with minimal antibacterial effects (MIC 256 μg/mL); however, it showed no inhibitory effects on pre-existing biofilm. Further, the 10058-F4 treatment suppressed the expression of sarA, the biofilm master regulator, along with biofilm genes, such as icaA, fnb, nuc, and sspA. Additionally, the results showed that 10058-F4 synergistically enhanced the antibacterial activity of norfloxacin and tetracycline, indicating its potential use as an adjunct to the existing antibiotic treatments. While these findings suggest the potential of 10058-F4 for clinical use, further investigations are necessary to elucidate its mechanism of action and optimize its application in combination therapies. |
| format | Article |
| id | doaj-art-be4bd5f596b14f3ba6cd7e2f5fb1fd7e |
| institution | Matheson Library |
| issn | 2590-2075 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-be4bd5f596b14f3ba6cd7e2f5fb1fd7e2025-07-21T04:10:04ZengElsevierBiofilm2590-20752025-12-011010030710058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureusHiren Dodia0Suvendu Ojha1Puja Chatterjee2Tushar Kant Beuria3Department of Infectious Disease Biology, Institute of Life Sciences, NALCO Square, Chandrasekharpur, Bhubaneswar, Odisha, 751023, India; Regional Centre for Biotechnology, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad Rd, Faridabad, Haryana, 121001, IndiaDepartment of Infectious Disease Biology, Institute of Life Sciences, NALCO Square, Chandrasekharpur, Bhubaneswar, Odisha, 751023, India; Regional Centre for Biotechnology, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad Rd, Faridabad, Haryana, 121001, IndiaDepartment of Infectious Disease Biology, Institute of Life Sciences, NALCO Square, Chandrasekharpur, Bhubaneswar, Odisha, 751023, India; Regional Centre for Biotechnology, 3rd Milestone, Faridabad-Gurgaon Expressway, Faridabad Rd, Faridabad, Haryana, 121001, IndiaDepartment of Infectious Disease Biology, Institute of Life Sciences, NALCO Square, Chandrasekharpur, Bhubaneswar, Odisha, 751023, India; Corresponding author.Antimicrobial resistance (AMR) is a global concern that undermines microbial disease treatment and prevention. WHO and World Bank's EcoAMR report predicts that AMR could cause 39 million deaths and $3.4 trillion in annual GDP losses by the year 2050. This is particularly critical with S. aureus, a cause of diverse infections like skin abscesses and pneumonia, where antibiotic resistance increases mortality and hinders treatment. Biofilms are one of the major causes of multi-drug resistance in S. aureus, and their inhibition can restore antibiotic sensitivity. In this study, through screening of the LOPAC drug library, we identified several compounds that exhibit biofilm inhibitory properties against multi-drug-resistant S. aureus without affecting its growth. The compound 10058-F4 was found to have the strongest anti-biofilm activity (>70 % inhibition) with minimal antibacterial effects (MIC 256 μg/mL); however, it showed no inhibitory effects on pre-existing biofilm. Further, the 10058-F4 treatment suppressed the expression of sarA, the biofilm master regulator, along with biofilm genes, such as icaA, fnb, nuc, and sspA. Additionally, the results showed that 10058-F4 synergistically enhanced the antibacterial activity of norfloxacin and tetracycline, indicating its potential use as an adjunct to the existing antibiotic treatments. While these findings suggest the potential of 10058-F4 for clinical use, further investigations are necessary to elucidate its mechanism of action and optimize its application in combination therapies.http://www.sciencedirect.com/science/article/pii/S2590207525000553Staphylococcus aureus10058-F4LOPAC drug libraryBiofilm inhibitorDrug repurposingAntimicrobial resistance (AMR) |
| spellingShingle | Hiren Dodia Suvendu Ojha Puja Chatterjee Tushar Kant Beuria 10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus Biofilm Staphylococcus aureus 10058-F4 LOPAC drug library Biofilm inhibitor Drug repurposing Antimicrobial resistance (AMR) |
| title | 10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus |
| title_full | 10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus |
| title_fullStr | 10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus |
| title_full_unstemmed | 10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus |
| title_short | 10058-F4 Mediated inhibition of the biofilm formation in multidrug-resistant Staphylococcus aureus |
| title_sort | 10058 f4 mediated inhibition of the biofilm formation in multidrug resistant staphylococcus aureus |
| topic | Staphylococcus aureus 10058-F4 LOPAC drug library Biofilm inhibitor Drug repurposing Antimicrobial resistance (AMR) |
| url | http://www.sciencedirect.com/science/article/pii/S2590207525000553 |
| work_keys_str_mv | AT hirendodia 10058f4mediatedinhibitionofthebiofilmformationinmultidrugresistantstaphylococcusaureus AT suvenduojha 10058f4mediatedinhibitionofthebiofilmformationinmultidrugresistantstaphylococcusaureus AT pujachatterjee 10058f4mediatedinhibitionofthebiofilmformationinmultidrugresistantstaphylococcusaureus AT tusharkantbeuria 10058f4mediatedinhibitionofthebiofilmformationinmultidrugresistantstaphylococcusaureus |