Purification of <i>Mogroside V</i> from Crude Extract of <i>Siraitia grosvenorii</i> Using Boronic Acid-Functionalized Silica Gel and Its Hypoglycemic Activity Determination
<i>Mogroside V</i> crude extract from <i>Siraitia grosvenorii</i> has many pharmacological effects, such as anti-diabetes, antioxidant, etc. It is being used as a kind of natural sweetener in more and more countries. The improvement of <i>Mogroside V</i> purity ca...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2297-8739/12/6/135 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <i>Mogroside V</i> crude extract from <i>Siraitia grosvenorii</i> has many pharmacological effects, such as anti-diabetes, antioxidant, etc. It is being used as a kind of natural sweetener in more and more countries. The improvement of <i>Mogroside V</i> purity can greatly promote the utilization value of <i>Siraitia grosvenorii</i> and the quality of related products. For this paper, a boronic acid-functionalized silica gel adsorbent (SiO<sub>2</sub>-GP-APBA) was synthesized and applied for the first time in the purification of <i>mogroside V</i> from the crude extract of <i>Siraitia grosvenorii</i>. It was demonstrated that it was 30–100 μm with 163.1 μmol/g of boronic acid groups on the surface of silica gel and stable at below 380.20 °C. Its maximum adsorption capacity to <i>mogroside V</i> was up to 206.74 mg/g at room temperature. After the saturated absorption from the crude extract of <i>Siraitia grosvenorii</i> in a pH 3 solution, 96.36% <i>mogroside V</i> could be released from SiO<sub>2</sub>-GP-APBA using a pH 7 aqueous solution, which was better than ethanol. The purity of <i>mogroside V</i> was significantly increased from 35.67% to 76.34%. Semi-preparative HPLC could further improve the purity <i>of mogroside V</i> to 99.60%. Additionally, the direct inhibition effect of the <i>mogroside V</i> on α-glucosidase was determined for the first time. Its inhibitory constant was 46.11 μM, indicating <i>mogroside V</i> was beneficial for the treatment of diabetes. |
|---|---|
| ISSN: | 2297-8739 |