Electrochemical Disposable Printed Aptasensor for Sensitive Ciprofloxacin Monitoring in Milk Samples
An electrochemical aptasensor was developed for the rapid and sensitive detection of ciprofloxacin (CPX) in milk samples. The device was fabricated on a polyethylene terephthalate (PET) substrate using a screen-printing technique with carbon-based conductive ink. Gold nanoparticles (AuNPs) were inco...
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MDPI AG
2025-06-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/13/7/235 |
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| author | Daniela Nunes da Silva Thaís Cristina de Oliveira Cândido Arnaldo César Pereira |
| author_facet | Daniela Nunes da Silva Thaís Cristina de Oliveira Cândido Arnaldo César Pereira |
| author_sort | Daniela Nunes da Silva |
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| description | An electrochemical aptasensor was developed for the rapid and sensitive detection of ciprofloxacin (CPX) in milk samples. The device was fabricated on a polyethylene terephthalate (PET) substrate using a screen-printing technique with carbon-based conductive ink. Gold nanoparticles (AuNPs) were incorporated to enhance aptamer immobilization and facilitate electron transfer at the electrode surface. The sensor’s analytical performance was optimized by adjusting key parameters, including AuNP volume, DNA aptamer concentration, and incubation times for both the aptamer and the blocking agent (6-mercapto-1-hexanol, MCH). Differential pulse voltammetry (DPV) measurements demonstrated a linear response ranging from 10 to 50 nmol L<sup>−1</sup> and a low detection limit of 3.0 nmol L<sup>−1</sup>. When applied to real milk samples, the method achieved high recovery rates (101.4–106.7%) with a relative standard deviation below 3.1%, confirming its robustness. This disposable and cost-effective aptasensor represents a promising tool for food safety monitoring, with potential for adaptation to detect other pharmaceutical residues in dairy products. |
| format | Article |
| id | doaj-art-bd6fa65b6a924d78af4b5ed092ceed1c |
| institution | Matheson Library |
| issn | 2227-9040 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Chemosensors |
| spelling | doaj-art-bd6fa65b6a924d78af4b5ed092ceed1c2025-07-25T13:18:01ZengMDPI AGChemosensors2227-90402025-06-0113723510.3390/chemosensors13070235Electrochemical Disposable Printed Aptasensor for Sensitive Ciprofloxacin Monitoring in Milk SamplesDaniela Nunes da Silva0Thaís Cristina de Oliveira Cândido1Arnaldo César Pereira2Natural Sciences Department, Federal University of São João del Rei, 74 Praça Dom Helvécio, São João del Rei 36301-160, MG, BrazilNatural Sciences Department, Federal University of São João del Rei, 74 Praça Dom Helvécio, São João del Rei 36301-160, MG, BrazilNatural Sciences Department, Federal University of São João del Rei, 74 Praça Dom Helvécio, São João del Rei 36301-160, MG, BrazilAn electrochemical aptasensor was developed for the rapid and sensitive detection of ciprofloxacin (CPX) in milk samples. The device was fabricated on a polyethylene terephthalate (PET) substrate using a screen-printing technique with carbon-based conductive ink. Gold nanoparticles (AuNPs) were incorporated to enhance aptamer immobilization and facilitate electron transfer at the electrode surface. The sensor’s analytical performance was optimized by adjusting key parameters, including AuNP volume, DNA aptamer concentration, and incubation times for both the aptamer and the blocking agent (6-mercapto-1-hexanol, MCH). Differential pulse voltammetry (DPV) measurements demonstrated a linear response ranging from 10 to 50 nmol L<sup>−1</sup> and a low detection limit of 3.0 nmol L<sup>−1</sup>. When applied to real milk samples, the method achieved high recovery rates (101.4–106.7%) with a relative standard deviation below 3.1%, confirming its robustness. This disposable and cost-effective aptasensor represents a promising tool for food safety monitoring, with potential for adaptation to detect other pharmaceutical residues in dairy products.https://www.mdpi.com/2227-9040/13/7/235electrochemical aptasensorscreen-printed electrodesgold nanoparticlesciprofloxacinmilk |
| spellingShingle | Daniela Nunes da Silva Thaís Cristina de Oliveira Cândido Arnaldo César Pereira Electrochemical Disposable Printed Aptasensor for Sensitive Ciprofloxacin Monitoring in Milk Samples Chemosensors electrochemical aptasensor screen-printed electrodes gold nanoparticles ciprofloxacin milk |
| title | Electrochemical Disposable Printed Aptasensor for Sensitive Ciprofloxacin Monitoring in Milk Samples |
| title_full | Electrochemical Disposable Printed Aptasensor for Sensitive Ciprofloxacin Monitoring in Milk Samples |
| title_fullStr | Electrochemical Disposable Printed Aptasensor for Sensitive Ciprofloxacin Monitoring in Milk Samples |
| title_full_unstemmed | Electrochemical Disposable Printed Aptasensor for Sensitive Ciprofloxacin Monitoring in Milk Samples |
| title_short | Electrochemical Disposable Printed Aptasensor for Sensitive Ciprofloxacin Monitoring in Milk Samples |
| title_sort | electrochemical disposable printed aptasensor for sensitive ciprofloxacin monitoring in milk samples |
| topic | electrochemical aptasensor screen-printed electrodes gold nanoparticles ciprofloxacin milk |
| url | https://www.mdpi.com/2227-9040/13/7/235 |
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