Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics
Summary: Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain are now available. However, few effective approaches enable both optical and mechanical access to the brain. Here, we present a protocol for synthesizing, implanting, and using polydimet...
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| Language: | English |
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Elsevier
2025-09-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003703 |
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| author | Kengo Takahashi Gerjan Huis in ‘t Veld Davide Benedetti Jun-Ying Wang Samuel Pontes Quero Rafael Yuste Cyriel M.A. Pennartz Umberto Olcese |
| author_facet | Kengo Takahashi Gerjan Huis in ‘t Veld Davide Benedetti Jun-Ying Wang Samuel Pontes Quero Rafael Yuste Cyriel M.A. Pennartz Umberto Olcese |
| author_sort | Kengo Takahashi |
| collection | DOAJ |
| description | Summary: Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain are now available. However, few effective approaches enable both optical and mechanical access to the brain. Here, we present a protocol for synthesizing, implanting, and using polydimethylsiloxane (PDMS) windows as skull replacements for chronic wide-field and two-photon calcium imaging in mice. We also describe steps for performing viral injections and multi-site silicon probe implantation. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-bc9e36c77c1e4d649ac3f9ee60040ad1 |
| institution | Matheson Library |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-bc9e36c77c1e4d649ac3f9ee60040ad12025-07-23T05:25:05ZengElsevierSTAR Protocols2666-16672025-09-0163103964Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogeneticsKengo Takahashi0Gerjan Huis in ‘t Veld1Davide Benedetti2Jun-Ying Wang3Samuel Pontes Quero4Rafael Yuste5Cyriel M.A. Pennartz6Umberto Olcese7Swammerdam Institute for Life Sciences, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Amsterdam Brain and Cognition, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Corresponding authorSwammerdam Institute for Life Sciences, University of Amsterdam, 1098XH Amsterdam, the NetherlandsSwammerdam Institute for Life Sciences, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Department of Translational Research and of New Surgical and Medical Technologies, University of Pisa, 56124 Pisa, ItalySwammerdam Institute for Life Sciences, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Amsterdam Brain and Cognition, University of Amsterdam, 1098XH Amsterdam, the NetherlandsNeuroTechnology Center, Department of Biological Sciences, Columbia University, New York City, NY 10027, USANeuroTechnology Center, Department of Biological Sciences, Columbia University, New York City, NY 10027, USASwammerdam Institute for Life Sciences, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Amsterdam Brain and Cognition, University of Amsterdam, 1098XH Amsterdam, the NetherlandsSwammerdam Institute for Life Sciences, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Amsterdam Brain and Cognition, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Corresponding authorSummary: Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain are now available. However, few effective approaches enable both optical and mechanical access to the brain. Here, we present a protocol for synthesizing, implanting, and using polydimethylsiloxane (PDMS) windows as skull replacements for chronic wide-field and two-photon calcium imaging in mice. We also describe steps for performing viral injections and multi-site silicon probe implantation. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725003703MicroscopyNeuroscienceCognitive NeuroscienceBehavior |
| spellingShingle | Kengo Takahashi Gerjan Huis in ‘t Veld Davide Benedetti Jun-Ying Wang Samuel Pontes Quero Rafael Yuste Cyriel M.A. Pennartz Umberto Olcese Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics STAR Protocols Microscopy Neuroscience Cognitive Neuroscience Behavior |
| title | Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics |
| title_full | Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics |
| title_fullStr | Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics |
| title_full_unstemmed | Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics |
| title_short | Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging, electrophysiology, and optogenetics |
| title_sort | protocol for synthesizing implanting and using polydimethylsiloxane as skull replacement in mice for imaging electrophysiology and optogenetics |
| topic | Microscopy Neuroscience Cognitive Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725003703 |
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