Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation of <i>Suaeda glauca</i> and <i>Phragmites communis</i> to Saline–Alkali Stress
While rhizosphere microbiome functions in saline soils are well documented, complementary microbial strategies between pioneer and late-successional halophytes remain unexplored. Here, we used 16S rRNA sequencing and FAPROTAX functional prediction to compare the rhizosphere bacterial communities of...
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2025-06-01
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| author | Hao Dai Mingyun Jia Jianhui Xue Zhuangzhuang Liu Dongqin Zhou Zhaoqi Hou Jinping Yu Shipeng Lu |
| author_facet | Hao Dai Mingyun Jia Jianhui Xue Zhuangzhuang Liu Dongqin Zhou Zhaoqi Hou Jinping Yu Shipeng Lu |
| author_sort | Hao Dai |
| collection | DOAJ |
| description | While rhizosphere microbiome functions in saline soils are well documented, complementary microbial strategies between pioneer and late-successional halophytes remain unexplored. Here, we used 16S rRNA sequencing and FAPROTAX functional prediction to compare the rhizosphere bacterial communities of two key halophytes—<i>Suaeda glauca</i> and <i>Phragmites communis</i>—in a reclaimed coastal wetland. The results demonstrate that both plants significantly restructured microbial communities through convergent enrichment of stress-tolerant taxa (<i>Firmicutes</i>, <i>Pseudomonas</i>, <i>Bacillus</i>, and <i>Planococcus</i>) while suppressing sulfur-oxidizing bacteria (<i>Sulfurovum</i> and <i>Thiobacillus</i>). However, they exhibited distinct microbial specialization: <i>S. glauca</i> uniquely enriched organic-matter-degrading taxa (<i>Promicromonospora</i> and <i>Zhihengliuella</i>) and upregulated aromatic compound degradation (2.29%) and ureolysis (0.86%) according to FAPROTAX analysis, facilitating carbon mobilization in early successional stages. Notably, <i>P. communis</i> selectively recruited nitrogen-cycling <i>Serratia</i>, with increased nitrate respiration (3.51% in <i>P. communis</i> vs. 0.91% in <i>S. glauca</i>) function, reflecting its higher nitrogen demand. Environmental factors also diverged: <i>S. glauca</i>’s microbiome correlated with potassium and sodium, whereas <i>P. communis</i> responded to phosphorus and chloride. These findings uncover distinct microbial recruitment strategies by halophytes to combat saline stress—<i>S. glauca</i>–<i>P. communis</i> synergy through microbial carbon-nitrogen coupling—offering a template for consortia design in saline soil restoration. |
| format | Article |
| id | doaj-art-bc68d926e11f4c51b89f3f96182570a4 |
| institution | Matheson Library |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-06-01 |
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| series | Microorganisms |
| spelling | doaj-art-bc68d926e11f4c51b89f3f96182570a42025-06-25T14:12:47ZengMDPI AGMicroorganisms2076-26072025-06-01136139910.3390/microorganisms13061399Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation of <i>Suaeda glauca</i> and <i>Phragmites communis</i> to Saline–Alkali StressHao Dai0Mingyun Jia1Jianhui Xue2Zhuangzhuang Liu3Dongqin Zhou4Zhaoqi Hou5Jinping Yu6Shipeng Lu7Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaWhile rhizosphere microbiome functions in saline soils are well documented, complementary microbial strategies between pioneer and late-successional halophytes remain unexplored. Here, we used 16S rRNA sequencing and FAPROTAX functional prediction to compare the rhizosphere bacterial communities of two key halophytes—<i>Suaeda glauca</i> and <i>Phragmites communis</i>—in a reclaimed coastal wetland. The results demonstrate that both plants significantly restructured microbial communities through convergent enrichment of stress-tolerant taxa (<i>Firmicutes</i>, <i>Pseudomonas</i>, <i>Bacillus</i>, and <i>Planococcus</i>) while suppressing sulfur-oxidizing bacteria (<i>Sulfurovum</i> and <i>Thiobacillus</i>). However, they exhibited distinct microbial specialization: <i>S. glauca</i> uniquely enriched organic-matter-degrading taxa (<i>Promicromonospora</i> and <i>Zhihengliuella</i>) and upregulated aromatic compound degradation (2.29%) and ureolysis (0.86%) according to FAPROTAX analysis, facilitating carbon mobilization in early successional stages. Notably, <i>P. communis</i> selectively recruited nitrogen-cycling <i>Serratia</i>, with increased nitrate respiration (3.51% in <i>P. communis</i> vs. 0.91% in <i>S. glauca</i>) function, reflecting its higher nitrogen demand. Environmental factors also diverged: <i>S. glauca</i>’s microbiome correlated with potassium and sodium, whereas <i>P. communis</i> responded to phosphorus and chloride. These findings uncover distinct microbial recruitment strategies by halophytes to combat saline stress—<i>S. glauca</i>–<i>P. communis</i> synergy through microbial carbon-nitrogen coupling—offering a template for consortia design in saline soil restoration.https://www.mdpi.com/2076-2607/13/6/1399<i>Suaeda glauca</i><i>Phragmites communis</i>rhizosphere microbiomesaline–alkali soilfunctional specializationhalophyte adaptation |
| spellingShingle | Hao Dai Mingyun Jia Jianhui Xue Zhuangzhuang Liu Dongqin Zhou Zhaoqi Hou Jinping Yu Shipeng Lu Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation of <i>Suaeda glauca</i> and <i>Phragmites communis</i> to Saline–Alkali Stress Microorganisms <i>Suaeda glauca</i> <i>Phragmites communis</i> rhizosphere microbiome saline–alkali soil functional specialization halophyte adaptation |
| title | Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation of <i>Suaeda glauca</i> and <i>Phragmites communis</i> to Saline–Alkali Stress |
| title_full | Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation of <i>Suaeda glauca</i> and <i>Phragmites communis</i> to Saline–Alkali Stress |
| title_fullStr | Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation of <i>Suaeda glauca</i> and <i>Phragmites communis</i> to Saline–Alkali Stress |
| title_full_unstemmed | Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation of <i>Suaeda glauca</i> and <i>Phragmites communis</i> to Saline–Alkali Stress |
| title_short | Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation of <i>Suaeda glauca</i> and <i>Phragmites communis</i> to Saline–Alkali Stress |
| title_sort | complementary rhizosphere microbial strategies drive functional specialization in coastal halophyte succession differential adaptation of i suaeda glauca i and i phragmites communis i to saline alkali stress |
| topic | <i>Suaeda glauca</i> <i>Phragmites communis</i> rhizosphere microbiome saline–alkali soil functional specialization halophyte adaptation |
| url | https://www.mdpi.com/2076-2607/13/6/1399 |
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