Rapid and sensitive detection of Mycobacterium tuberculosis using the RPA/Cas12f1_ge4.1 system with fluorescence and lateral flow readouts
ABSTRACT Tuberculosis remains a major global health threat, with existing detection methods often limited by efficiency and resource demands. Our previous PAM-dependent dsDNA Target-activated Cas12f1 Trans Reporter (PDTCTR) fluorescence sensing platform, while effective for PAM-dependent pathogen de...
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Main Authors: | Zhongliang Deng, Xingyong Weng, Honghua Tang, Tintao Zou, Xuan Zhou, Hangxi Liu, Piaoting Wen, Gemiao Luo, Tian Gan, Jun He |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2025-07-01
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Series: | Microbiology Spectrum |
Subjects: | |
Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02652-24 |
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