A novel device for buffy coat collection
Collection of the buffy coat layer from whole blood is critical for detecting rare circulating cells, such as circulating tumor cells (CTCs), which are of great diagnostic and research importance. Conventional methods for buffy coat collection often have low yields, significant erythrocyte contamina...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-07-01
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| Series: | BioTechniques |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07366205.2025.2534299 |
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| _version_ | 1839621789877010432 |
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| author | Kyle Christian Hardy Lisa Arrigo Jennifer Campbell Jacob Fiedler Paul Fiedler |
| author_facet | Kyle Christian Hardy Lisa Arrigo Jennifer Campbell Jacob Fiedler Paul Fiedler |
| author_sort | Kyle Christian Hardy |
| collection | DOAJ |
| description | Collection of the buffy coat layer from whole blood is critical for detecting rare circulating cells, such as circulating tumor cells (CTCs), which are of great diagnostic and research importance. Conventional methods for buffy coat collection often have low yields, significant erythrocyte contamination, and/or high costs limiting their utility. We developed a novel, multichannel aspiration device that provides efficient buffy coat collection with minimal erythrocyte contamination. This study employed spiked-in myeloma cells to model CTCs and evaluate device performance across a range of CTC concentrations (12, 30, and 300 cells/mL). The device demonstrated high CTC recovery rates, achieving up to 98% at high CTC concentrations and 89% at low concentrations. Immunofluorescent imaging confirmed preservation of cell morphology throughout the collection process. This convenient technology offers the potential of a low-cost alternative for buffy coat collection to be utilized in a wide range of clinical and research applications. |
| format | Article |
| id | doaj-art-bbd7b5c8b5e14b3c85bc4e61d14ea6ad |
| institution | Matheson Library |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-bbd7b5c8b5e14b3c85bc4e61d14ea6ad2025-07-22T11:42:53ZengTaylor & Francis GroupBioTechniques0736-62051940-98182025-07-011810.1080/07366205.2025.2534299A novel device for buffy coat collectionKyle Christian Hardy0Lisa Arrigo1Jennifer Campbell2Jacob Fiedler3Paul Fiedler4Rudy L. Ruggles Biomedical Research Institute, Nuvance Health, Danbury, CT, USARudy L. Ruggles Biomedical Research Institute, Nuvance Health, Danbury, CT, USARudy L. Ruggles Biomedical Research Institute, Nuvance Health, Danbury, CT, USANew York Medical College, Tuoro University System, Valhalla, NY, USARudy L. Ruggles Biomedical Research Institute, Nuvance Health, Danbury, CT, USACollection of the buffy coat layer from whole blood is critical for detecting rare circulating cells, such as circulating tumor cells (CTCs), which are of great diagnostic and research importance. Conventional methods for buffy coat collection often have low yields, significant erythrocyte contamination, and/or high costs limiting their utility. We developed a novel, multichannel aspiration device that provides efficient buffy coat collection with minimal erythrocyte contamination. This study employed spiked-in myeloma cells to model CTCs and evaluate device performance across a range of CTC concentrations (12, 30, and 300 cells/mL). The device demonstrated high CTC recovery rates, achieving up to 98% at high CTC concentrations and 89% at low concentrations. Immunofluorescent imaging confirmed preservation of cell morphology throughout the collection process. This convenient technology offers the potential of a low-cost alternative for buffy coat collection to be utilized in a wide range of clinical and research applications.https://www.tandfonline.com/doi/10.1080/07366205.2025.2534299Buffy coat collectioncirculating tumor cellsaspiration deviceliquid biopsycell recovery efficiency |
| spellingShingle | Kyle Christian Hardy Lisa Arrigo Jennifer Campbell Jacob Fiedler Paul Fiedler A novel device for buffy coat collection BioTechniques Buffy coat collection circulating tumor cells aspiration device liquid biopsy cell recovery efficiency |
| title | A novel device for buffy coat collection |
| title_full | A novel device for buffy coat collection |
| title_fullStr | A novel device for buffy coat collection |
| title_full_unstemmed | A novel device for buffy coat collection |
| title_short | A novel device for buffy coat collection |
| title_sort | novel device for buffy coat collection |
| topic | Buffy coat collection circulating tumor cells aspiration device liquid biopsy cell recovery efficiency |
| url | https://www.tandfonline.com/doi/10.1080/07366205.2025.2534299 |
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