STUDY OF THERMAL INACTIVATION CLADOSPORIUM CLADOSPORIOIDES α-GALACTOSIDASE
Thermostability of Cladosporium cladosporioides of α-galactosidase was studied in the range of 55 °С and 60 °С. Some of the thermodynamic parameters have been estimated and the mechanism of thermal inactivation has been suggested. Activation energy of transition α-galactosidase subunit from native t...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Odesa I. I. Mechnikov National University
2010-03-01
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| Series: | Mìkrobìologìâ ì Bìotehnologìâ. Мікробіологія і біотехнологія |
| Subjects: | |
| Online Access: | http://mbt.onu.edu.ua/article/view/98370 |
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| Summary: | Thermostability of Cladosporium cladosporioides of α-galactosidase was studied in the range of 55 °С and 60 °С. Some of the thermodynamic parameters have been estimated and the mechanism of thermal inactivation has been suggested. Activation energy of transition α-galactosidase subunit from native to denatured state was 165 kJ/M. Stabilization of enzyme molecule in the presence of BSA (19%), glycerol (30–40%), galactose (15%), melibiose, raffinose and stachyose (45%, 21% and 15%) was shown. |
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| ISSN: | 2076-0558 2307-4663 |