Study on culturing Trichodema mutants
Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone+chlorothalonil, or maneb or phosethyl-Al). The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 2...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.04.0443 |
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| Summary: | Trichodema mutants strains T5, T0803, T1010, T1003were cultured in different conditions and media, also in the presence of fungicides at 40 mg/kg (CK or procymidone+chlorothalonil, or maneb or phosethyl-Al). The pH values of media were 5, 6, 7 and 8 and hyphae were grown at temperatures of 15, 20, 25 and 30 ℃. After being cultured for 3, 4, 5, or 6 days, the strains were transferred at a lower temperature to sporulate (20 ℃). Obtained data were analyzed statistically, with the orthogonal array and ranges (R) differing dependes on the treatments (R=40.0,42.4, 48.0, 62.8, 107.0). The results indicated that the most important factor was the nature of the strain (R=107.0), while the change in temperature and time of cultivation produced the lowest effect (R=40.0). Each factor variance was significant and A<sub>3</sub>B<sub>4</sub>C<sub>2</sub>D<sub>1</sub>E<sub>3</sub> was the optimum combined condition, in which strain T1010 grew more quickly and sporulated most. |
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| ISSN: | 1008-9209 2097-5155 |