Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virus
Soybean mosaic virus (SMV) is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, RSC4, RSC8, and RSC14Q, have been identified and mapped on soybean chromosomes 14, 2, and 13 from Dabaima, Kefeng 1, and Qihuang 1 cultivars, respectively. Soybean cultivar N...
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KeAi Communications Co., Ltd.
2017-11-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311917616824 |
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| author | Da-gang WANG Lin ZHAO Kai LI Ying MA Li-qun WANG Yong-qing YANG Yun-hua YANG Hai-jian ZHI |
| author_facet | Da-gang WANG Lin ZHAO Kai LI Ying MA Li-qun WANG Yong-qing YANG Yun-hua YANG Hai-jian ZHI |
| author_sort | Da-gang WANG |
| collection | DOAJ |
| description | Soybean mosaic virus (SMV) is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, RSC4, RSC8, and RSC14Q, have been identified and mapped on soybean chromosomes 14, 2, and 13 from Dabaima, Kefeng 1, and Qihuang 1 cultivars, respectively. Soybean cultivar Nannong 1138-2 is widely grown in the Yangtze River Valley of China. In this study, crosses were made between Qihuang 1×Kefeng 1 and Dabaima×Nannong 1138-2. Ten simple sequence repeat (SSR) markers linked to three resistance loci (RSC4, RSC8, and RSC14Q) were used to assist pyramided breeding. Pyramided families containing three resistance loci (RSC4, RSC8, and RSC14Q) were evaluated by inoculating them with 21 SMV strains from China. Results indicated that the 10 markers can be used effectively to assist the selection of resistant individuals containing RSC4, RSC8, and RSC14Q A total of 53 F6 plants were confirmed to contain three homozygous alleles conferring resistance to SMV. Five F7 homozygous pyramided families exhibited resistance to 21 strains of SMV and showed desirable agronomic traits using dual selection. The strategy of pyramiding resistance gene derived from different varieties has practical breeding value in providing broad-spectrum resistance against the existing strains of SMV in China. |
| format | Article |
| id | doaj-art-bb2edc3695ca4496a96e31a8644fc1a6 |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2017-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-bb2edc3695ca4496a96e31a8644fc1a62025-08-02T17:56:14ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192017-11-01161124132420Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virusDa-gang WANG0Lin ZHAO1Kai LI2Ying MA3Li-qun WANG4Yong-qing YANG5Yun-hua YANG6Hai-jian ZHI7College of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.China; Crop Institute, Anhui Academy of Agricultural Sciences/Anhui Key Laboratory of Crops Quality Improving, Hefei 230031, P.R.China; WANG Da-gangCollege of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.ChinaCollege of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.ChinaCollege of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.ChinaCollege of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.ChinaCollege of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.ChinaCollege of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.ChinaCollege of Agriculture, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.China; Correspondence ZHI Hai-jian, Tel: +86-25-84396463Soybean mosaic virus (SMV) is one of the major viral pathogens affecting soybean crops worldwide. Three SMV resistance genes, RSC4, RSC8, and RSC14Q, have been identified and mapped on soybean chromosomes 14, 2, and 13 from Dabaima, Kefeng 1, and Qihuang 1 cultivars, respectively. Soybean cultivar Nannong 1138-2 is widely grown in the Yangtze River Valley of China. In this study, crosses were made between Qihuang 1×Kefeng 1 and Dabaima×Nannong 1138-2. Ten simple sequence repeat (SSR) markers linked to three resistance loci (RSC4, RSC8, and RSC14Q) were used to assist pyramided breeding. Pyramided families containing three resistance loci (RSC4, RSC8, and RSC14Q) were evaluated by inoculating them with 21 SMV strains from China. Results indicated that the 10 markers can be used effectively to assist the selection of resistant individuals containing RSC4, RSC8, and RSC14Q A total of 53 F6 plants were confirmed to contain three homozygous alleles conferring resistance to SMV. Five F7 homozygous pyramided families exhibited resistance to 21 strains of SMV and showed desirable agronomic traits using dual selection. The strategy of pyramiding resistance gene derived from different varieties has practical breeding value in providing broad-spectrum resistance against the existing strains of SMV in China.http://www.sciencedirect.com/science/article/pii/S2095311917616824soybeansoybean mosaic virusresistance genespyramidingmarker-assisted breeding |
| spellingShingle | Da-gang WANG Lin ZHAO Kai LI Ying MA Li-qun WANG Yong-qing YANG Yun-hua YANG Hai-jian ZHI Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virus Journal of Integrative Agriculture soybean soybean mosaic virus resistance genes pyramiding marker-assisted breeding |
| title | Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virus |
| title_full | Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virus |
| title_fullStr | Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virus |
| title_full_unstemmed | Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virus |
| title_short | Marker-assisted pyramiding of soybean resistance genes RSC4, RSC8, and RSC14Q to soybean mosaic virus |
| title_sort | marker assisted pyramiding of soybean resistance genes rsc4 rsc8 and rsc14q to soybean mosaic virus |
| topic | soybean soybean mosaic virus resistance genes pyramiding marker-assisted breeding |
| url | http://www.sciencedirect.com/science/article/pii/S2095311917616824 |
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