Construction and application of a novel multiple target plasmid used for identification of 18 genetically modified soybean transformants
A novel multiple target plasmid (MTP) molecule that satisfies the identification of specific transgenic soybean transformants in China was constructed. Fourteen transformants approved for import into China and four ones unauthorized but have important application prospects, were chosen as the target...
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| Príomhchruthaitheoirí: | , , , , , , , , , , , , |
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| Formáid: | Alt |
| Teanga: | Béarla |
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Zhejiang University Press
2020-06-01
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| Sraith: | 浙江大学学报. 农业与生命科学版 |
| Ábhair: | |
| Rochtain ar líne: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2019.08.122 |
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| Achoimre: | A novel multiple target plasmid (MTP) molecule that satisfies the identification of specific transgenic soybean transformants in China was constructed. Fourteen transformants approved for import into China and four ones unauthorized but have important application prospects, were chosen as the targets. The transformants’ specific sequences described in corresponding national detection standard and the detection sequence of the soybean endogenous reference gene Lectin were properly arranged and spliced, and then was inserted to linearized pUC18 plasmid to produce a multi-target plasmid pDDID-1905. Among the insertion sequences, plenty of restriction endonuclease recognition sites were dispersed uniquely, which could be used for later revision and/or update of the plasmid. Polymerase chain reaction (PCR) detection targeted on the 19 inserted sequences was performed to verify the utility of the plasmid. The results showed that all target sequences were successfully amplified and the products met the expected sizes, which indicated that the multi-target plasmid pDDID-1905 suitable for the identification of 18 transgenic transformants was constructed. Due to the widest target coverage of pDDID-1905, it will greatly simplify the cumbersome preparation of positive materials for identifying these transgenic soybean transformants and their derivates. |
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| ISSN: | 1008-9209 2097-5155 |