Effects of combo therapy with coenzyme Q10 and mitochondrial transplantation on myocardial ischemia/reperfusion-induced arrhythmias in aged rats
Objective(s): Ischemia/reperfusion (IR)-induced ventricular arrhythmia, which mainly occurs after the opening of coronary artery occlusion, poses a clinical problem. This study aims to investigate the effectiveness of pretreatment with coenzyme Q10 (CoQ10) in combination with mitochondrial transplan...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2025-01-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_24884_141a83170627ab7898f5e2953d44c982.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839613316178116608 |
|---|---|
| author | Soleyman Bafadam Behnaz Mokhtari Alireza Alihemmati Reza Badalzadeh |
| author_facet | Soleyman Bafadam Behnaz Mokhtari Alireza Alihemmati Reza Badalzadeh |
| author_sort | Soleyman Bafadam |
| collection | DOAJ |
| description | Objective(s): Ischemia/reperfusion (IR)-induced ventricular arrhythmia, which mainly occurs after the opening of coronary artery occlusion, poses a clinical problem. This study aims to investigate the effectiveness of pretreatment with coenzyme Q10 (CoQ10) in combination with mitochondrial transplantation on IR-induced ventricular arrhythmias in aged rats.Materials and Methods: Myocardial IR induction was performed by left anterior descending coronary artery occlusion for 30 min, followed by re-opening for 24 hr. CoQ10 was administered intraperitoneally at a dosage of 10 mg/kg/day for two weeks before inducing IR. At the start of reperfusion, 500 µl of the respiration buffer containing 6×106±5×105 mitochondria/ml of respiration buffer harvested from the pectorals major muscle of young donor rats were injected intramyocardially. To investigate arrhythmias, the heart’s electrical activity during ischemia and the first 30 min of reperfusion were recorded by electrocardiogram. After 24 hr of reperfusion, cardiac histopathological changes, creatine kinase-MB, nitric oxide metabolites (NOx), oxidative stress markers (malondialdehyde, total anti-oxidant, superoxide dismutase, and glutathione peroxidase), and the expression of genes regulating mitochondrial fission/fusion were measured.Results: Pretreatment with CoQ10 in combination with mitochondrial transplantation reduced ventricular arrhythmias, cardiac histopathological changes, and creatine kinase-MB levels. Simultaneously, this combined therapeutic approach increased myocardial NOx levels, fostering an improved oxidative balance. It also triggered the down-regulation of mitochondrial fission genes, coupled with the up-regulation of mitochondrial fusion genes. Conclusion: The combination of CoQ10 and mitochondrial transplantation demonstrated a notable anti-arrhythmic effect by elevating NOx levels, reducing oxidative stress, and improving mitochondrial fission/fusion in aged rats with myocardial IRI. |
| format | Article |
| id | doaj-art-b9a4013ecd2e49b489e4eb7c7c9726c0 |
| institution | Matheson Library |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-b9a4013ecd2e49b489e4eb7c7c9726c02025-07-27T09:51:52ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-01-01281384810.22038/ijbms.2024.80092.1734824884Effects of combo therapy with coenzyme Q10 and mitochondrial transplantation on myocardial ischemia/reperfusion-induced arrhythmias in aged ratsSoleyman Bafadam0Behnaz Mokhtari1Alireza Alihemmati2Reza Badalzadeh3Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2 Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranMolecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, IranObjective(s): Ischemia/reperfusion (IR)-induced ventricular arrhythmia, which mainly occurs after the opening of coronary artery occlusion, poses a clinical problem. This study aims to investigate the effectiveness of pretreatment with coenzyme Q10 (CoQ10) in combination with mitochondrial transplantation on IR-induced ventricular arrhythmias in aged rats.Materials and Methods: Myocardial IR induction was performed by left anterior descending coronary artery occlusion for 30 min, followed by re-opening for 24 hr. CoQ10 was administered intraperitoneally at a dosage of 10 mg/kg/day for two weeks before inducing IR. At the start of reperfusion, 500 µl of the respiration buffer containing 6×106±5×105 mitochondria/ml of respiration buffer harvested from the pectorals major muscle of young donor rats were injected intramyocardially. To investigate arrhythmias, the heart’s electrical activity during ischemia and the first 30 min of reperfusion were recorded by electrocardiogram. After 24 hr of reperfusion, cardiac histopathological changes, creatine kinase-MB, nitric oxide metabolites (NOx), oxidative stress markers (malondialdehyde, total anti-oxidant, superoxide dismutase, and glutathione peroxidase), and the expression of genes regulating mitochondrial fission/fusion were measured.Results: Pretreatment with CoQ10 in combination with mitochondrial transplantation reduced ventricular arrhythmias, cardiac histopathological changes, and creatine kinase-MB levels. Simultaneously, this combined therapeutic approach increased myocardial NOx levels, fostering an improved oxidative balance. It also triggered the down-regulation of mitochondrial fission genes, coupled with the up-regulation of mitochondrial fusion genes. Conclusion: The combination of CoQ10 and mitochondrial transplantation demonstrated a notable anti-arrhythmic effect by elevating NOx levels, reducing oxidative stress, and improving mitochondrial fission/fusion in aged rats with myocardial IRI.https://ijbms.mums.ac.ir/article_24884_141a83170627ab7898f5e2953d44c982.pdfarrhythmiacoenzyme q10mitochondriamyocardial ischemia/reperfusion injuryoxidative stress |
| spellingShingle | Soleyman Bafadam Behnaz Mokhtari Alireza Alihemmati Reza Badalzadeh Effects of combo therapy with coenzyme Q10 and mitochondrial transplantation on myocardial ischemia/reperfusion-induced arrhythmias in aged rats Iranian Journal of Basic Medical Sciences arrhythmia coenzyme q10 mitochondria myocardial ischemia/reperfusion injury oxidative stress |
| title | Effects of combo therapy with coenzyme Q10 and mitochondrial transplantation on myocardial ischemia/reperfusion-induced arrhythmias in aged rats |
| title_full | Effects of combo therapy with coenzyme Q10 and mitochondrial transplantation on myocardial ischemia/reperfusion-induced arrhythmias in aged rats |
| title_fullStr | Effects of combo therapy with coenzyme Q10 and mitochondrial transplantation on myocardial ischemia/reperfusion-induced arrhythmias in aged rats |
| title_full_unstemmed | Effects of combo therapy with coenzyme Q10 and mitochondrial transplantation on myocardial ischemia/reperfusion-induced arrhythmias in aged rats |
| title_short | Effects of combo therapy with coenzyme Q10 and mitochondrial transplantation on myocardial ischemia/reperfusion-induced arrhythmias in aged rats |
| title_sort | effects of combo therapy with coenzyme q10 and mitochondrial transplantation on myocardial ischemia reperfusion induced arrhythmias in aged rats |
| topic | arrhythmia coenzyme q10 mitochondria myocardial ischemia/reperfusion injury oxidative stress |
| url | https://ijbms.mums.ac.ir/article_24884_141a83170627ab7898f5e2953d44c982.pdf |
| work_keys_str_mv | AT soleymanbafadam effectsofcombotherapywithcoenzymeq10andmitochondrialtransplantationonmyocardialischemiareperfusioninducedarrhythmiasinagedrats AT behnazmokhtari effectsofcombotherapywithcoenzymeq10andmitochondrialtransplantationonmyocardialischemiareperfusioninducedarrhythmiasinagedrats AT alirezaalihemmati effectsofcombotherapywithcoenzymeq10andmitochondrialtransplantationonmyocardialischemiareperfusioninducedarrhythmiasinagedrats AT rezabadalzadeh effectsofcombotherapywithcoenzymeq10andmitochondrialtransplantationonmyocardialischemiareperfusioninducedarrhythmiasinagedrats |