Effects of DTX3L on the cell proliferation, adhesion, and drug resistance of multiple myeloma cells
Cell adhesion–mediated drug resistance is an important factor that influences the effects of chemotherapy in multiple myeloma. DTX3L, a ubiquitin ligase, plays a key role in cell-cycle-related process. Here, we found that the expression of DTX3L gradually increased during the proliferation of myelom...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2017-06-01
|
| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317703941 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839598074031243264 |
|---|---|
| author | Yaodong Shen Yuxiang Sun Linlin Zhang Hong Liu |
| author_facet | Yaodong Shen Yuxiang Sun Linlin Zhang Hong Liu |
| author_sort | Yaodong Shen |
| collection | DOAJ |
| description | Cell adhesion–mediated drug resistance is an important factor that influences the effects of chemotherapy in multiple myeloma. DTX3L, a ubiquitin ligase, plays a key role in cell-cycle-related process. Here, we found that the expression of DTX3L gradually increased during the proliferation of myeloma cells, which resulted in arrest of the cell cycle in the G1 phase and promoted the adherence of myeloma cells to fibronectin or bone marrow stromal cells. In addition, silencing of DTX3L improved sensitivity to chemotherapy drugs in multiple myeloma cell lines adherent to bone marrow stromal cells and increased the expression of caspase-3 and poly-adenosine diphosphate-ribose polymerase, two markers of apoptosis. Finally, we also found that DTX3L expression was regulated by focal adhesion kinase. Taken together, the results of this study show that DTX3L plays an important role in the proliferation and cell adhesion–mediated drug resistance of multiple myeloma cells and as such may play a key role in the development of multiple myeloma. |
| format | Article |
| id | doaj-art-b78c1baac5d44e9ab7ff755ae7af7eb0 |
| institution | Matheson Library |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-b78c1baac5d44e9ab7ff755ae7af7eb02025-08-02T17:35:04ZengSAGE PublishingTumor Biology1423-03802017-06-013910.1177/1010428317703941Effects of DTX3L on the cell proliferation, adhesion, and drug resistance of multiple myeloma cellsYaodong Shen0Yuxiang Sun1Linlin Zhang2Hong Liu3Department of Hematology, Affiliated Hospital of Nantong University, Nantong, People’s Republic of ChinaJiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, People’s Republic of ChinaJiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, People’s Republic of ChinaDepartment of Hematology, Affiliated Hospital of Nantong University, Nantong, People’s Republic of ChinaCell adhesion–mediated drug resistance is an important factor that influences the effects of chemotherapy in multiple myeloma. DTX3L, a ubiquitin ligase, plays a key role in cell-cycle-related process. Here, we found that the expression of DTX3L gradually increased during the proliferation of myeloma cells, which resulted in arrest of the cell cycle in the G1 phase and promoted the adherence of myeloma cells to fibronectin or bone marrow stromal cells. In addition, silencing of DTX3L improved sensitivity to chemotherapy drugs in multiple myeloma cell lines adherent to bone marrow stromal cells and increased the expression of caspase-3 and poly-adenosine diphosphate-ribose polymerase, two markers of apoptosis. Finally, we also found that DTX3L expression was regulated by focal adhesion kinase. Taken together, the results of this study show that DTX3L plays an important role in the proliferation and cell adhesion–mediated drug resistance of multiple myeloma cells and as such may play a key role in the development of multiple myeloma.https://doi.org/10.1177/1010428317703941 |
| spellingShingle | Yaodong Shen Yuxiang Sun Linlin Zhang Hong Liu Effects of DTX3L on the cell proliferation, adhesion, and drug resistance of multiple myeloma cells Tumor Biology |
| title | Effects of DTX3L on the cell proliferation, adhesion, and drug resistance of multiple myeloma cells |
| title_full | Effects of DTX3L on the cell proliferation, adhesion, and drug resistance of multiple myeloma cells |
| title_fullStr | Effects of DTX3L on the cell proliferation, adhesion, and drug resistance of multiple myeloma cells |
| title_full_unstemmed | Effects of DTX3L on the cell proliferation, adhesion, and drug resistance of multiple myeloma cells |
| title_short | Effects of DTX3L on the cell proliferation, adhesion, and drug resistance of multiple myeloma cells |
| title_sort | effects of dtx3l on the cell proliferation adhesion and drug resistance of multiple myeloma cells |
| url | https://doi.org/10.1177/1010428317703941 |
| work_keys_str_mv | AT yaodongshen effectsofdtx3lonthecellproliferationadhesionanddrugresistanceofmultiplemyelomacells AT yuxiangsun effectsofdtx3lonthecellproliferationadhesionanddrugresistanceofmultiplemyelomacells AT linlinzhang effectsofdtx3lonthecellproliferationadhesionanddrugresistanceofmultiplemyelomacells AT hongliu effectsofdtx3lonthecellproliferationadhesionanddrugresistanceofmultiplemyelomacells |