Integrated Transcriptomic and Physiological Analysis Reveals the Drought Adaptation Strategies of <i>Sapindus delavayi</i>, an Important Tree for Industrial Use in the Dry–Hot Valleys
<i>Sapindus delavayi</i> is a drought-resistant tree species endemic to the dry–hot valleys of Southwestern China and is of great significance for soil and water conservation and ecological restoration. In this study, we sequenced the transcriptome of its leaves using the Illumina HiSeq...
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2025-05-01
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| author | Xinyu He Meng Xie Lan Wang Liangjun Yu Fengjuan Li Hong Ma |
| author_facet | Xinyu He Meng Xie Lan Wang Liangjun Yu Fengjuan Li Hong Ma |
| author_sort | Xinyu He |
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| description | <i>Sapindus delavayi</i> is a drought-resistant tree species endemic to the dry–hot valleys of Southwestern China and is of great significance for soil and water conservation and ecological restoration. In this study, we sequenced the transcriptome of its leaves using the Illumina HiSeq 4000 platform and obtained 96.12 Gb of high-quality data (Q20 = 98.68%, Q30 = 95.62%), which were <i>de novo</i> assembled to obtain 89,228 unigenes (N50 = 1538 bp), of which 63,005 (70.61%) were successfully annotated to at least one database (NR, NT, SwissProt, KOG, KEGG, GO, Pfam). Overall, 53.96% of the unigenes in the <i>S. delavayi</i> leaves were annotated to <i>Acer yangbiense</i>, which belongs to the same family as <i>S. delavayi</i>. A total of 42,870 CDSs and 21,488 SSR loci were detected, with the highest mononucleotide repeat rate at 42.72% of the total number. Drought stress experiments identified 669–1203 differentially expressed genes (DEGs). Through our research, the first high-quality transcriptome database of <i>S. delavayi</i> has been constructed and its drought-resistance-related gene features have been analyzed, laying an important foundation for future functional gene mining, molecular marker development, molecular diversity studies, molecular breeding, and ecological adaptation research. |
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| publishDate | 2025-05-01 |
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| spelling | doaj-art-b7318dba9b744955a1b0a080e1e7dbfc2025-06-25T13:55:11ZengMDPI AGHorticulturae2311-75242025-05-0111660310.3390/horticulturae11060603Integrated Transcriptomic and Physiological Analysis Reveals the Drought Adaptation Strategies of <i>Sapindus delavayi</i>, an Important Tree for Industrial Use in the Dry–Hot ValleysXinyu He0Meng Xie1Lan Wang2Liangjun Yu3Fengjuan Li4Hong Ma5Institute of Highland Forest Science, Chinese Academy of Forestry, State Key Laboratory of Efficient Production of Forest Resources, Kunming 650216, ChinaInstitute of Highland Forest Science, Chinese Academy of Forestry, State Key Laboratory of Efficient Production of Forest Resources, Kunming 650216, ChinaYunnan Xianggu Biotechnology Co., Ltd., Kunming 650102, ChinaEditorial Department of Journal, Yunnan Agricultural University, Kunming 650201, ChinaInstitute of Highland Forest Science, Chinese Academy of Forestry, State Key Laboratory of Efficient Production of Forest Resources, Kunming 650216, ChinaInstitute of Highland Forest Science, Chinese Academy of Forestry, State Key Laboratory of Efficient Production of Forest Resources, Kunming 650216, China<i>Sapindus delavayi</i> is a drought-resistant tree species endemic to the dry–hot valleys of Southwestern China and is of great significance for soil and water conservation and ecological restoration. In this study, we sequenced the transcriptome of its leaves using the Illumina HiSeq 4000 platform and obtained 96.12 Gb of high-quality data (Q20 = 98.68%, Q30 = 95.62%), which were <i>de novo</i> assembled to obtain 89,228 unigenes (N50 = 1538 bp), of which 63,005 (70.61%) were successfully annotated to at least one database (NR, NT, SwissProt, KOG, KEGG, GO, Pfam). Overall, 53.96% of the unigenes in the <i>S. delavayi</i> leaves were annotated to <i>Acer yangbiense</i>, which belongs to the same family as <i>S. delavayi</i>. A total of 42,870 CDSs and 21,488 SSR loci were detected, with the highest mononucleotide repeat rate at 42.72% of the total number. Drought stress experiments identified 669–1203 differentially expressed genes (DEGs). Through our research, the first high-quality transcriptome database of <i>S. delavayi</i> has been constructed and its drought-resistance-related gene features have been analyzed, laying an important foundation for future functional gene mining, molecular marker development, molecular diversity studies, molecular breeding, and ecological adaptation research.https://www.mdpi.com/2311-7524/11/6/603<i>Sapindus delavayi</i>transcriptome analysisfunctional annotationdrought stress response |
| spellingShingle | Xinyu He Meng Xie Lan Wang Liangjun Yu Fengjuan Li Hong Ma Integrated Transcriptomic and Physiological Analysis Reveals the Drought Adaptation Strategies of <i>Sapindus delavayi</i>, an Important Tree for Industrial Use in the Dry–Hot Valleys Horticulturae <i>Sapindus delavayi</i> transcriptome analysis functional annotation drought stress response |
| title | Integrated Transcriptomic and Physiological Analysis Reveals the Drought Adaptation Strategies of <i>Sapindus delavayi</i>, an Important Tree for Industrial Use in the Dry–Hot Valleys |
| title_full | Integrated Transcriptomic and Physiological Analysis Reveals the Drought Adaptation Strategies of <i>Sapindus delavayi</i>, an Important Tree for Industrial Use in the Dry–Hot Valleys |
| title_fullStr | Integrated Transcriptomic and Physiological Analysis Reveals the Drought Adaptation Strategies of <i>Sapindus delavayi</i>, an Important Tree for Industrial Use in the Dry–Hot Valleys |
| title_full_unstemmed | Integrated Transcriptomic and Physiological Analysis Reveals the Drought Adaptation Strategies of <i>Sapindus delavayi</i>, an Important Tree for Industrial Use in the Dry–Hot Valleys |
| title_short | Integrated Transcriptomic and Physiological Analysis Reveals the Drought Adaptation Strategies of <i>Sapindus delavayi</i>, an Important Tree for Industrial Use in the Dry–Hot Valleys |
| title_sort | integrated transcriptomic and physiological analysis reveals the drought adaptation strategies of i sapindus delavayi i an important tree for industrial use in the dry hot valleys |
| topic | <i>Sapindus delavayi</i> transcriptome analysis functional annotation drought stress response |
| url | https://www.mdpi.com/2311-7524/11/6/603 |
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