Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma
Objective The development of self nano emulsifying co-delivery system of doxorubicin and Nigella sativa oil for potentiating the anticancer effects against HepG2 cell lines.Materials and methods SNEDDS were formulated by using Labrafil and N. sativa oil (3:2% w/w), Kolliphor RH40 (15% w/w), glycerol...
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1581791 |
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| author | Afreen Usmani Anuradha Mishra Md Arshad Asif Jafri |
| author_facet | Afreen Usmani Anuradha Mishra Md Arshad Asif Jafri |
| author_sort | Afreen Usmani |
| collection | DOAJ |
| description | Objective The development of self nano emulsifying co-delivery system of doxorubicin and Nigella sativa oil for potentiating the anticancer effects against HepG2 cell lines.Materials and methods SNEDDS were formulated by using Labrafil and N. sativa oil (3:2% w/w), Kolliphor RH40 (15% w/w), glycerol (5% w/w) as oil phase, surfactant and co-surfactant while deionized water (75% v/v) used as an aqueous phase. Optimized SNEDDS was evaluated for drug release and in vitro anticancer efficacy in liver cancer (HepG2) cell line.Results and discussion The selected formulation (F6) has a mean particle size of 79.7 nm with PDI 0.098 and the minimum viscosity of 16.42 cps with % transmittance of 1.332 with maximum drug release of 96.968% in 32 h as compared to DOX alone. Stability data showed stable emulsion in both 250C and -40C. F6 showed improved efficacy in HepG2 cells by cytotoxicity, showed significant results p<.05 with 2.5 μg/ml of (inhibitory concentration) IC50.Conclusion The overall study displayed that co-delivery of DOX and Nigella sativa oil in the form of SNEDDS may be an efficient carrier for further in vivo studies using oral delivery in human hepatocellular carcinoma in mammals. |
| format | Article |
| id | doaj-art-b6ece908b87e4e3f9da11b2e8ec53013 |
| institution | Matheson Library |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-b6ece908b87e4e3f9da11b2e8ec530132025-07-21T21:20:32ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-0147193394410.1080/21691401.2019.1581791Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinomaAfreen Usmani0Anuradha Mishra1Md Arshad2Asif Jafri3Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, IndiaFaculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, IndiaMolecular Endocrinology Lab, Department of Zoology, University of Lucknow, Lucknow, Uttar Pradesh, IndiaMolecular Endocrinology Lab, Department of Zoology, University of Lucknow, Lucknow, Uttar Pradesh, IndiaObjective The development of self nano emulsifying co-delivery system of doxorubicin and Nigella sativa oil for potentiating the anticancer effects against HepG2 cell lines.Materials and methods SNEDDS were formulated by using Labrafil and N. sativa oil (3:2% w/w), Kolliphor RH40 (15% w/w), glycerol (5% w/w) as oil phase, surfactant and co-surfactant while deionized water (75% v/v) used as an aqueous phase. Optimized SNEDDS was evaluated for drug release and in vitro anticancer efficacy in liver cancer (HepG2) cell line.Results and discussion The selected formulation (F6) has a mean particle size of 79.7 nm with PDI 0.098 and the minimum viscosity of 16.42 cps with % transmittance of 1.332 with maximum drug release of 96.968% in 32 h as compared to DOX alone. Stability data showed stable emulsion in both 250C and -40C. F6 showed improved efficacy in HepG2 cells by cytotoxicity, showed significant results p<.05 with 2.5 μg/ml of (inhibitory concentration) IC50.Conclusion The overall study displayed that co-delivery of DOX and Nigella sativa oil in the form of SNEDDS may be an efficient carrier for further in vivo studies using oral delivery in human hepatocellular carcinoma in mammals.https://www.tandfonline.com/doi/10.1080/21691401.2019.1581791DoxorubicinNigella sativacell viabilitynuclear fragmentationROS |
| spellingShingle | Afreen Usmani Anuradha Mishra Md Arshad Asif Jafri Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma Artificial Cells, Nanomedicine, and Biotechnology Doxorubicin Nigella sativa cell viability nuclear fragmentation ROS |
| title | Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma |
| title_full | Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma |
| title_fullStr | Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma |
| title_full_unstemmed | Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma |
| title_short | Development and evaluation of doxorubicin self nanoemulsifying drug delivery system with Nigella Sativa oil against human hepatocellular carcinoma |
| title_sort | development and evaluation of doxorubicin self nanoemulsifying drug delivery system with nigella sativa oil against human hepatocellular carcinoma |
| topic | Doxorubicin Nigella sativa cell viability nuclear fragmentation ROS |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1581791 |
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