Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae Family
The pharmacological potential of Lamiaceae plants is primarily linked to their high content of phenolic acids and flavonoids, known for strong antioxidant properties. This study investigated the antioxidant activity of ten widely used Lamiaceae herbs—oregano, lavender, basil, savory, garden thyme, w...
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2025-06-01
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| author | Branislava Teofilović Emilia Gligorić Martina Ninić Saša Vukmirović Žarko Gagić Nebojša Mandić-Kovačević Biljana Tubić Đorđe Đukanović Nevena Grujić-Letić |
| author_facet | Branislava Teofilović Emilia Gligorić Martina Ninić Saša Vukmirović Žarko Gagić Nebojša Mandić-Kovačević Biljana Tubić Đorđe Đukanović Nevena Grujić-Letić |
| author_sort | Branislava Teofilović |
| collection | DOAJ |
| description | The pharmacological potential of Lamiaceae plants is primarily linked to their high content of phenolic acids and flavonoids, known for strong antioxidant properties. This study investigated the antioxidant activity of ten widely used Lamiaceae herbs—oregano, lavender, basil, savory, garden thyme, wild thyme, sage, rosemary, lemon balm, and mint—prepared as traditional infusions and microwave-assisted extracts. The antioxidant capacity was evaluated using spectrophotometric assays, and total phenolics and flavonoids were quantified via spectrophotometry and HPLC. Chemometric analysis (PCA) was applied to explore correlations among antioxidant parameters. The results demonstrated excellent antioxidant activity across all samples. The IC50 for DPPH radicals was in the range from 3.73(0.13) to 8.03(0.17) μg/mL and that for ABTS radicals was from 2.89(0.12) to 8.55(0.34). The CUPRAC antioxidant assay delivered values in the range from 351.93(11.85) to 1129.68(44.46) μg TE/mg DE. The FRAP method produced values from 1.27(0.03) to 6.60(0.26) μmol Fe/mg DE. The presence of gallic acid was detected in all examined samples, with lemon balm and lavender exhibiting the highest concentrations across both applied extraction methods. Notably, lavender showed especially high levels of p-hydroxybenzoic acid and chlorogenic acid. Microwave-assisted extraction generally yielded higher levels of bioactive compounds compared to infusion. These findings highlight the potential of Lamiaceae herbal extracts, particularly those obtained through microwave-assisted extraction, as valuable sources of dietary antioxidants for everyday use. |
| format | Article |
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| institution | Matheson Library |
| issn | 2297-8739 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-b64c005f73ad44d68b40d8bc14ea24bb2025-06-25T14:25:57ZengMDPI AGSeparations2297-87392025-06-0112615510.3390/separations12060155Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae FamilyBranislava Teofilović0Emilia Gligorić1Martina Ninić2Saša Vukmirović3Žarko Gagić4Nebojša Mandić-Kovačević5Biljana Tubić6Đorđe Đukanović7Nevena Grujić-Letić8Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, SerbiaFaculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, SerbiaFaculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, SerbiaFaculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, SerbiaFaculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Bosnia and HerzegovinaFaculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Bosnia and HerzegovinaFaculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Bosnia and HerzegovinaFaculty of Medicine, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Bosnia and HerzegovinaFaculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, SerbiaThe pharmacological potential of Lamiaceae plants is primarily linked to their high content of phenolic acids and flavonoids, known for strong antioxidant properties. This study investigated the antioxidant activity of ten widely used Lamiaceae herbs—oregano, lavender, basil, savory, garden thyme, wild thyme, sage, rosemary, lemon balm, and mint—prepared as traditional infusions and microwave-assisted extracts. The antioxidant capacity was evaluated using spectrophotometric assays, and total phenolics and flavonoids were quantified via spectrophotometry and HPLC. Chemometric analysis (PCA) was applied to explore correlations among antioxidant parameters. The results demonstrated excellent antioxidant activity across all samples. The IC50 for DPPH radicals was in the range from 3.73(0.13) to 8.03(0.17) μg/mL and that for ABTS radicals was from 2.89(0.12) to 8.55(0.34). The CUPRAC antioxidant assay delivered values in the range from 351.93(11.85) to 1129.68(44.46) μg TE/mg DE. The FRAP method produced values from 1.27(0.03) to 6.60(0.26) μmol Fe/mg DE. The presence of gallic acid was detected in all examined samples, with lemon balm and lavender exhibiting the highest concentrations across both applied extraction methods. Notably, lavender showed especially high levels of p-hydroxybenzoic acid and chlorogenic acid. Microwave-assisted extraction generally yielded higher levels of bioactive compounds compared to infusion. These findings highlight the potential of Lamiaceae herbal extracts, particularly those obtained through microwave-assisted extraction, as valuable sources of dietary antioxidants for everyday use.https://www.mdpi.com/2297-8739/12/6/155Lamiaceaeantioxidant activityherbal teaschemometric analysisinfusionmicrowave-assisted extraction |
| spellingShingle | Branislava Teofilović Emilia Gligorić Martina Ninić Saša Vukmirović Žarko Gagić Nebojša Mandić-Kovačević Biljana Tubić Đorđe Đukanović Nevena Grujić-Letić Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae Family Separations Lamiaceae antioxidant activity herbal teas chemometric analysis infusion microwave-assisted extraction |
| title | Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae Family |
| title_full | Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae Family |
| title_fullStr | Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae Family |
| title_full_unstemmed | Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae Family |
| title_short | Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae Family |
| title_sort | green extraction combined with chemometric approach profiling phytochemicals and antioxidant properties of ten species of the lamiaceae family |
| topic | Lamiaceae antioxidant activity herbal teas chemometric analysis infusion microwave-assisted extraction |
| url | https://www.mdpi.com/2297-8739/12/6/155 |
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