Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors
The rapid and precise identification of multiple pathogens is critical for ensuring food safety, controlling epidemics, diagnosing diseases, and monitoring the environment. However, traditional detection methods are hindered by complex workflows, the need for skilled operators, and reliance on sophi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/6/378 |
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| _version_ | 1839654611348094976 |
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| author | Yue Wu Xing Xu Yinchu Zhu Jiaxin Wan Xingbo Wang Xin Zhou Xiangjun Li Weidong Zhou |
| author_facet | Yue Wu Xing Xu Yinchu Zhu Jiaxin Wan Xingbo Wang Xin Zhou Xiangjun Li Weidong Zhou |
| author_sort | Yue Wu |
| collection | DOAJ |
| description | The rapid and precise identification of multiple pathogens is critical for ensuring food safety, controlling epidemics, diagnosing diseases, and monitoring the environment. However, traditional detection methods are hindered by complex workflows, the need for skilled operators, and reliance on sophisticated equipment, making them unsuitable for rapid, on-site testing. Optical biosensors, known for their rapid analysis, portability, high sensitivity, and multiplexing capabilities, offer a promising solution for simultaneous multi-pathogenic identification. This paper explores recent advancements in the utilization of optical biosensors for multiple pathogenic detection. First, it provides an overview of key sensing principles, focusing on colorimetric, fluorescence-based, surface-enhanced Raman scattering (SERS), and surface plasmon resonance (SPR) techniques, as well as their applications in pathogenic detection. Then, the research progress and practical applications of optical biosensors for multiplex pathogenic detection are discussed in detail from three perspectives: microfluidic devices, nucleic acid amplification technology (NAAT), and nanomaterials. Finally, the challenges presented by optical biosensing technologies in multi-pathogen detection are discussed, along with future prospects and potential innovations in the field. |
| format | Article |
| id | doaj-art-b51c0c2a1b9b401787d21bd85d6ff501 |
| institution | Matheson Library |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-b51c0c2a1b9b401787d21bd85d6ff5012025-06-25T13:34:09ZengMDPI AGBiosensors2079-63742025-06-0115637810.3390/bios15060378Research Progress on Multiplexed Pathogen Detection Using Optical BiosensorsYue Wu0Xing Xu1Yinchu Zhu2Jiaxin Wan3Xingbo Wang4Xin Zhou5Xiangjun Li6Weidong Zhou7State Key Laboratory for Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCollege of Biological and Environmental Science, Zhejiang Wanli University, Ningbo 315100, ChinaState Key Laboratory for Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCollege of Information Engineering, China Jiliang University, Hangzhou 314423, ChinaState Key Laboratory for Quality and Safety of Agro-Products, Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThe rapid and precise identification of multiple pathogens is critical for ensuring food safety, controlling epidemics, diagnosing diseases, and monitoring the environment. However, traditional detection methods are hindered by complex workflows, the need for skilled operators, and reliance on sophisticated equipment, making them unsuitable for rapid, on-site testing. Optical biosensors, known for their rapid analysis, portability, high sensitivity, and multiplexing capabilities, offer a promising solution for simultaneous multi-pathogenic identification. This paper explores recent advancements in the utilization of optical biosensors for multiple pathogenic detection. First, it provides an overview of key sensing principles, focusing on colorimetric, fluorescence-based, surface-enhanced Raman scattering (SERS), and surface plasmon resonance (SPR) techniques, as well as their applications in pathogenic detection. Then, the research progress and practical applications of optical biosensors for multiplex pathogenic detection are discussed in detail from three perspectives: microfluidic devices, nucleic acid amplification technology (NAAT), and nanomaterials. Finally, the challenges presented by optical biosensing technologies in multi-pathogen detection are discussed, along with future prospects and potential innovations in the field.https://www.mdpi.com/2079-6374/15/6/378multiple pathogensoptical biosensorsmicrofluidic devicesnucleic acid amplificationnanomaterials |
| spellingShingle | Yue Wu Xing Xu Yinchu Zhu Jiaxin Wan Xingbo Wang Xin Zhou Xiangjun Li Weidong Zhou Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors Biosensors multiple pathogens optical biosensors microfluidic devices nucleic acid amplification nanomaterials |
| title | Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors |
| title_full | Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors |
| title_fullStr | Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors |
| title_full_unstemmed | Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors |
| title_short | Research Progress on Multiplexed Pathogen Detection Using Optical Biosensors |
| title_sort | research progress on multiplexed pathogen detection using optical biosensors |
| topic | multiple pathogens optical biosensors microfluidic devices nucleic acid amplification nanomaterials |
| url | https://www.mdpi.com/2079-6374/15/6/378 |
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