Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation
BackgroundDonation-related infections (DRIs), particularly those caused by carbapenem-resistant gram-negative bacteria (CRGNB), can have disastrous consequences because of their extensive drug resistance. Contamination during graft acquisition and transport can lead to DRIs, and the use of antibioti...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-07-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1572799/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839619562459365376 |
|---|---|
| author | Yazhe Duan Junhao Yu Pei Zhang Yuhong Li Kewen Chen Yanfeng Li Yuxiang Wan Kang Wu Li Zeng Yanhua Li Mingxing Sui |
| author_facet | Yazhe Duan Junhao Yu Pei Zhang Yuhong Li Kewen Chen Yanfeng Li Yuxiang Wan Kang Wu Li Zeng Yanhua Li Mingxing Sui |
| author_sort | Yazhe Duan |
| collection | DOAJ |
| description | BackgroundDonation-related infections (DRIs), particularly those caused by carbapenem-resistant gram-negative bacteria (CRGNB), can have disastrous consequences because of their extensive drug resistance. Contamination during graft acquisition and transport can lead to DRIs, and the use of antibiotics in preservation fluid (PF) before organ transplantation can reduce the incidence of DRIs. This study was to determine and compare the effectiveness of different PF decontamination regimens to prevent CRGNB related DRIs.MethodsTwelve CRGNB strains were chosen to be the targets of decontamination, and a drug concentration gradient was established for each test drug based on the previous clinical research. In addition the standard decontamination procedures were performed to evaluate the antimicrobial effectiveness of polymyxin B (PB), colistin sulfate (CS), colistimethate sodium (CMS) and amikacin (AK) in the 0~4°C PF, and to explore the antimicrobial effects of CMS after different preprocessing methods.ResultsPB and CS exhibited significantly better antimicrobial effectiveness against CRGNB than AK and CMS in the 0~4°C PF, and the antimicrobial effects on CRGNB increased with the increasing concentration of drugs. Notably, CMS after pretreatment (CMS-AP), its antibacterial was significantly enhanced at 4°C.ConclusionsThe PF decontamination is important in preventing the DRIs caused by CRGNB, and the decontamination regimens based on PB or CS were confirmed effective. Notably, CMS could even achieve a better decontamination effect than PB after a simple and fast pretreatment. |
| format | Article |
| id | doaj-art-b50cc4bca66f45a99638ae02fcdca51a |
| institution | Matheson Library |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-b50cc4bca66f45a99638ae02fcdca51a2025-07-23T05:35:41ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-07-011510.3389/fcimb.2025.15727991572799Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantationYazhe Duan0Junhao Yu1Pei Zhang2Yuhong Li3Kewen Chen4Yanfeng Li5Yuxiang Wan6Kang Wu7Li Zeng8Yanhua Li9Mingxing Sui10Department of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Laboratory Diagnostics, Shanghai Changhai Hospital, Shanghai, ChinaDepartment of Laboratory Diagnostics, Shanghai Changhai Hospital, Shanghai, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaDepartment of Organ Transplantation, The First Affiliated Hospital of Naval Medical University, Shanghai, ChinaBackgroundDonation-related infections (DRIs), particularly those caused by carbapenem-resistant gram-negative bacteria (CRGNB), can have disastrous consequences because of their extensive drug resistance. Contamination during graft acquisition and transport can lead to DRIs, and the use of antibiotics in preservation fluid (PF) before organ transplantation can reduce the incidence of DRIs. This study was to determine and compare the effectiveness of different PF decontamination regimens to prevent CRGNB related DRIs.MethodsTwelve CRGNB strains were chosen to be the targets of decontamination, and a drug concentration gradient was established for each test drug based on the previous clinical research. In addition the standard decontamination procedures were performed to evaluate the antimicrobial effectiveness of polymyxin B (PB), colistin sulfate (CS), colistimethate sodium (CMS) and amikacin (AK) in the 0~4°C PF, and to explore the antimicrobial effects of CMS after different preprocessing methods.ResultsPB and CS exhibited significantly better antimicrobial effectiveness against CRGNB than AK and CMS in the 0~4°C PF, and the antimicrobial effects on CRGNB increased with the increasing concentration of drugs. Notably, CMS after pretreatment (CMS-AP), its antibacterial was significantly enhanced at 4°C.ConclusionsThe PF decontamination is important in preventing the DRIs caused by CRGNB, and the decontamination regimens based on PB or CS were confirmed effective. Notably, CMS could even achieve a better decontamination effect than PB after a simple and fast pretreatment.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1572799/fulldonation-related infectionCRGNBdecontaminationpolymyxintransplant |
| spellingShingle | Yazhe Duan Junhao Yu Pei Zhang Yuhong Li Kewen Chen Yanfeng Li Yuxiang Wan Kang Wu Li Zeng Yanhua Li Mingxing Sui Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation Frontiers in Cellular and Infection Microbiology donation-related infection CRGNB decontamination polymyxin transplant |
| title | Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation |
| title_full | Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation |
| title_fullStr | Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation |
| title_full_unstemmed | Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation |
| title_short | Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation |
| title_sort | prevention of donation related infections investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation |
| topic | donation-related infection CRGNB decontamination polymyxin transplant |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1572799/full |
| work_keys_str_mv | AT yazheduan preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT junhaoyu preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT peizhang preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT yuhongli preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT kewenchen preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT yanfengli preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT yuxiangwan preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT kangwu preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT lizeng preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT yanhuali preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation AT mingxingsui preventionofdonationrelatedinfectionsinvestigatingtheuseofantibioticsinthedecontaminationofpreservationfluidfororgantransplantation |