Smoking Effect on Oxidative Stress and Antioxidant Capacity of Cervical Mucus in Women with Primary Idiopathic Sterility – Observational Case-Control Study
The aim of the study was to determine the effects of smoking on cervical mucus in women with primary idiopathic sterility. A prospective, case-control, observational, quantitative, and analytical study was carried out over a five-year period through ambulatory care. The first part of the study in...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sestre Milosrdnice University hospital, Institute of Clinical Medical Research
2024-01-01
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| Series: | Acta Clinica Croatica |
| Subjects: | |
| Online Access: | https://hrcak.srce.hr/file/481966 |
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| Summary: | The aim of the study was to determine the effects of smoking on cervical mucus
in women with primary idiopathic sterility. A prospective, case-control, observational, quantitative,
and analytical study was carried out over a five-year period through ambulatory care. The first part of
the study involved 140 healthy women (85 smokers and 55 non-smokers). The second part included
90 women (58 healthy, 32 suffering from sterility), grouped according to their smoking status. Concentrations
of total proteins in cervical mucus solutions and homogenates of cervical surface cells and
their mitochondria, thiocyanate and ascorbic acid, rhodanese activity, concentrations of cytochrome
P-450 (mitochondria), lipid peroxidation and the total antioxidant capacity in homogenates of cervical
surface cells, concentrations of superoxide O2, reduced gluthatione (GSH), advanced oxidation
protein products (AOPP), and ferric reducing ability of plasma (FRAP) were measured. The measurements
showed higher concentrations of thiocyanate, cytochrome P-450 and lipid peroxidation
values, and lower concentrations of ascorbic acid, cytochrome P-450 activity and FRAP in smokers.
Higher concentrations of superoxide O2, GSH, AOPP, and lower FRAP were found among smokers
and women suffering from sterility. In conclusion, changes in oxidative stress and antioxidant capacity
caused by smoking indicate that the quality of cervical mucus among patients suffering from sterility
is lower compared to healthy women. |
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| ISSN: | 0353-9466 1333-9451 |