Molecular characteristics and antimicrobial susceptibility of carbapenem-resistant Klebsiella pneumoniae in a multicenter study in Ningbo, China
ObjectiveTo analyze the molecular epidemiology and antimicrobial resistance profiles of carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolates in Ningbo, with the aim of providing a theoretical basis for hospital infection control strategies and the implementation of precise clinical diagnosis...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1628592/full |
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| author | Hongfei Shi Hongfei Shi Yanye Tu Hong Li Hui Gao Feng Wang Wei Zhang Min Jiang Qian Sun Zheng Bao Xiangwei Yang Yanzi Chang |
| author_facet | Hongfei Shi Hongfei Shi Yanye Tu Hong Li Hui Gao Feng Wang Wei Zhang Min Jiang Qian Sun Zheng Bao Xiangwei Yang Yanzi Chang |
| author_sort | Hongfei Shi |
| collection | DOAJ |
| description | ObjectiveTo analyze the molecular epidemiology and antimicrobial resistance profiles of carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolates in Ningbo, with the aim of providing a theoretical basis for hospital infection control strategies and the implementation of precise clinical diagnosis and treatment protocols.MethodsDuring the period from April 30, 2023 to June 30, 2024, clinical isolates of Klebsiella pneumoniae were collected from multiple centers in Ningbo, including The Affiliated Li Huili Hospital (Yinzhou District, Ningbo), Xiangshan Red Cross Taiwan Compatriot Hospital Medical and Health Group (Xiangshan, Ningbo), and the Second Hospital of Ninghai County (Ninghai, Ningbo). A total of 81 CR-KP strains were identified using the broth dilution method for carbapenem resistance screening. These isolates were submitted to Beijing Novo gene Co., Ltd. for sequencing analysis. The sequencing data were analyzed using online tools (https://bigsdb.pasteur.fr/ and http://genepi.food.dtu.dk/resfinder) to obtain information on multilocus sequence typing (MLST), capsular serotype (KL type), virulence genes, and resistance genes. Phylogenetic relationships were constructed using SNP software. For plasmid characterization, the PlasmidFinder online tool (https://cge.food.dtu.dk/services/PlasmidFinder/) was utilized to identify plasmid replicon genes and perform Inc. typing analysis. Furthermore, to conduct a comprehensive collinearity analysis of the blaKPC-2 resistance plasmid gene, gene cluster maps were constructed using Bakta v1.11.0 and Clinker v0.0.28 software packages.ResultsAmong the 81 CR-KP isolates, MLST typing revealed that ST11 was the predominant sequence type, accounting for 66.67% (54/81), with KL64 being the dominant capsular type. Among the non-ST11 CR-KP isolates, the ST15 type accounted for 48.15% (13/27), with KL19 being the predominant capsular serotype. The carriage rate of virulence genes—including rmpA2, fyuA, and 10 other genes—was significantly higher in ST11 CR-KP compared to non-ST11 CR-KP (p < 0.05). Analysis of resistance genes revealed that ST11 CR-KP primarily carried blaKPC-2 (100%, 54/54), whereas the resistance gene profiles among non-ST11 CR-KP isolates were more diverse, including blaNDM, blaIMP, and blaOXA. Plasmid typing indicated that ST11 CR-KP predominantly harbored IncFII (98.15%, 53/54) and RepB (72.22%, 39/54) plasmid types. In contrast, non-ST11 CR-KP isolates exhibited a wider range of plasmid types, including IncX3 (33.33%, 9/27), RepB (25.93%, 7/27), IncFII (25.93%, 7/27), IncFIB (7.41%, 2/27), and both ColKP3 and Col440II (7.41%, 2/27). Antimicrobial susceptibility testing demonstrated high resistance rates to commonly used antibiotics in both ST11 and non-ST11 CR-KP isolates. ST11 CR-KP exhibited 100% resistance to six antibiotics, including ceftriaxone (CRO), cefotetan (CTT), and cefepime (FEP), and showed susceptibility only to gentamicin (GEN), aztreonam/avibactam (AZA), ceftazidime/avibactam (CZA), polymyxin B (POL), and tigecycline (TGC). Non-ST11 CR-KP showed a significantly higher resistance rate to gentamicin (GEN) and ceftazidime/avibactam (CZA) than ST11 CR-KP (p < 0.05), but lower resistance rates to cefotetan (74.07%), all of which were statistically significant (p < 0.05).ConclusionIn the Ningbo region, CR-KP is predominantly of the ST11-KL64 type, exhibiting both strong antimicrobial resistance and high virulence characteristics. Non-ST11 CR-KP isolates carry genetically diverse carbapenemase genes and mobile genetic elements (e.g., IncX3, ColKP3). ST11 CR-KP strains demonstrate significantly stronger resistance profiles compared to non-ST11 strains. Therefore, stringent control over the use of carbapenem antibiotics is essential, along with measures to prevent the spread of resistance plasmids and the continuous improvement of hospital infection control strategies. |
| format | Article |
| id | doaj-art-b24a63b9c5a24b28b6a8544621c7c7b1 |
| institution | Matheson Library |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-b24a63b9c5a24b28b6a8544621c7c7b12025-07-21T06:05:52ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.16285921628592Molecular characteristics and antimicrobial susceptibility of carbapenem-resistant Klebsiella pneumoniae in a multicenter study in Ningbo, ChinaHongfei Shi0Hongfei Shi1Yanye Tu2Hong Li3Hui Gao4Feng Wang5Wei Zhang6Min Jiang7Qian Sun8Zheng Bao9Xiangwei Yang10Yanzi Chang11Department of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Ningbo Women and Children’s Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, Xiangshan Red Cross Taiwan Compatriot Hospital Medical and Health Group, Ningbo, ChinaDepartment of Clinical Laboratory, Second Hospital of Ninghai County, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated Li Huili Hospital of Ningbo University, Ningbo, ChinaObjectiveTo analyze the molecular epidemiology and antimicrobial resistance profiles of carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolates in Ningbo, with the aim of providing a theoretical basis for hospital infection control strategies and the implementation of precise clinical diagnosis and treatment protocols.MethodsDuring the period from April 30, 2023 to June 30, 2024, clinical isolates of Klebsiella pneumoniae were collected from multiple centers in Ningbo, including The Affiliated Li Huili Hospital (Yinzhou District, Ningbo), Xiangshan Red Cross Taiwan Compatriot Hospital Medical and Health Group (Xiangshan, Ningbo), and the Second Hospital of Ninghai County (Ninghai, Ningbo). A total of 81 CR-KP strains were identified using the broth dilution method for carbapenem resistance screening. These isolates were submitted to Beijing Novo gene Co., Ltd. for sequencing analysis. The sequencing data were analyzed using online tools (https://bigsdb.pasteur.fr/ and http://genepi.food.dtu.dk/resfinder) to obtain information on multilocus sequence typing (MLST), capsular serotype (KL type), virulence genes, and resistance genes. Phylogenetic relationships were constructed using SNP software. For plasmid characterization, the PlasmidFinder online tool (https://cge.food.dtu.dk/services/PlasmidFinder/) was utilized to identify plasmid replicon genes and perform Inc. typing analysis. Furthermore, to conduct a comprehensive collinearity analysis of the blaKPC-2 resistance plasmid gene, gene cluster maps were constructed using Bakta v1.11.0 and Clinker v0.0.28 software packages.ResultsAmong the 81 CR-KP isolates, MLST typing revealed that ST11 was the predominant sequence type, accounting for 66.67% (54/81), with KL64 being the dominant capsular type. Among the non-ST11 CR-KP isolates, the ST15 type accounted for 48.15% (13/27), with KL19 being the predominant capsular serotype. The carriage rate of virulence genes—including rmpA2, fyuA, and 10 other genes—was significantly higher in ST11 CR-KP compared to non-ST11 CR-KP (p < 0.05). Analysis of resistance genes revealed that ST11 CR-KP primarily carried blaKPC-2 (100%, 54/54), whereas the resistance gene profiles among non-ST11 CR-KP isolates were more diverse, including blaNDM, blaIMP, and blaOXA. Plasmid typing indicated that ST11 CR-KP predominantly harbored IncFII (98.15%, 53/54) and RepB (72.22%, 39/54) plasmid types. In contrast, non-ST11 CR-KP isolates exhibited a wider range of plasmid types, including IncX3 (33.33%, 9/27), RepB (25.93%, 7/27), IncFII (25.93%, 7/27), IncFIB (7.41%, 2/27), and both ColKP3 and Col440II (7.41%, 2/27). Antimicrobial susceptibility testing demonstrated high resistance rates to commonly used antibiotics in both ST11 and non-ST11 CR-KP isolates. ST11 CR-KP exhibited 100% resistance to six antibiotics, including ceftriaxone (CRO), cefotetan (CTT), and cefepime (FEP), and showed susceptibility only to gentamicin (GEN), aztreonam/avibactam (AZA), ceftazidime/avibactam (CZA), polymyxin B (POL), and tigecycline (TGC). Non-ST11 CR-KP showed a significantly higher resistance rate to gentamicin (GEN) and ceftazidime/avibactam (CZA) than ST11 CR-KP (p < 0.05), but lower resistance rates to cefotetan (74.07%), all of which were statistically significant (p < 0.05).ConclusionIn the Ningbo region, CR-KP is predominantly of the ST11-KL64 type, exhibiting both strong antimicrobial resistance and high virulence characteristics. Non-ST11 CR-KP isolates carry genetically diverse carbapenemase genes and mobile genetic elements (e.g., IncX3, ColKP3). ST11 CR-KP strains demonstrate significantly stronger resistance profiles compared to non-ST11 strains. Therefore, stringent control over the use of carbapenem antibiotics is essential, along with measures to prevent the spread of resistance plasmids and the continuous improvement of hospital infection control strategies.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1628592/fullKlebsiella pneumoniaecarbapenem resistanceresistance genesvirulence genesplasmids introduction |
| spellingShingle | Hongfei Shi Hongfei Shi Yanye Tu Hong Li Hui Gao Feng Wang Wei Zhang Min Jiang Qian Sun Zheng Bao Xiangwei Yang Yanzi Chang Molecular characteristics and antimicrobial susceptibility of carbapenem-resistant Klebsiella pneumoniae in a multicenter study in Ningbo, China Frontiers in Microbiology Klebsiella pneumoniae carbapenem resistance resistance genes virulence genes plasmids introduction |
| title | Molecular characteristics and antimicrobial susceptibility of carbapenem-resistant Klebsiella pneumoniae in a multicenter study in Ningbo, China |
| title_full | Molecular characteristics and antimicrobial susceptibility of carbapenem-resistant Klebsiella pneumoniae in a multicenter study in Ningbo, China |
| title_fullStr | Molecular characteristics and antimicrobial susceptibility of carbapenem-resistant Klebsiella pneumoniae in a multicenter study in Ningbo, China |
| title_full_unstemmed | Molecular characteristics and antimicrobial susceptibility of carbapenem-resistant Klebsiella pneumoniae in a multicenter study in Ningbo, China |
| title_short | Molecular characteristics and antimicrobial susceptibility of carbapenem-resistant Klebsiella pneumoniae in a multicenter study in Ningbo, China |
| title_sort | molecular characteristics and antimicrobial susceptibility of carbapenem resistant klebsiella pneumoniae in a multicenter study in ningbo china |
| topic | Klebsiella pneumoniae carbapenem resistance resistance genes virulence genes plasmids introduction |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1628592/full |
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