A quick-to-implement and optimized CRISPR-Cas9 protocol to obtain insertional and small indel mutants in Chlamydomonas reinhardtii

A quick-to-implement and optimized CRISPR-Cas9 protocol to obtain insertional and small indel mutants in Chlamydomonas reinhardtii

Chlamydomonas reinhardtii is a leading model organism in algal research, widely used to study photosynthesis, chloroplast and cilia biology, and more recently, metabolism, light signaling, the cell cycle, and algal biotechnology. Its sequenced genome has significantly accelerated research in the fie...

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Bibliographic Details
Main Authors: Mariano A. De Silvio, Camila Sánchez-Retuerta, M. Águila Ruiz-Sola, Olga Baidukova, Elena Monte
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:MethodsX
Subjects:
CRISPR gene editing and small indel detection in Chlamydomonas
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016125002626
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