Identification of glycosylated flavonoids as new physiological ligands of the hazel allergen Cor a 1: Complex structures reveal different binding orientation and specificity of mono- and disaccharide derivatives
The global production of hazelnuts is rising, but hazelnut allergies remain common and severe. The PR-10-like allergen Cor a 1, found in hazel pollen and nuts, consists of different isoallergens with over 67 % sequence identity. Cor a 1 binds plant secondary metabolites like the diglycosylated flavo...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-07-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525005589 |
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| Summary: | The global production of hazelnuts is rising, but hazelnut allergies remain common and severe. The PR-10-like allergen Cor a 1, found in hazel pollen and nuts, consists of different isoallergens with over 67 % sequence identity. Cor a 1 binds plant secondary metabolites like the diglycosylated flavonoid Q3O-(Glc)-Gal. Understanding the ligand binding of Cor a 1 isoallergens is crucial to understand their physiological and allergenic properties.We identified physiological mono- and diglycosylated flavonoids from hazel pollen by UHPLC/QTOF-MS and NMR spectroscopy and investigated their binding to Cor a 1 isoallergens by NMR HSQC experiments and ITC titrations. Screening analyses revealed different binding properties of isoallergens. Whereas the isoallergen Cor a 1.0501 bound only monoglycosylated flavonoids, Cor a 1.0401 also bound diglycosylated flavonoids. Protein/ligand complex structures based on NMR NOESY and computational docking experiments revealed different orientations of mono- vs. diglycosylated flavonoids in the amphiphilic pocket and specific binding of disaccharide derivatives. |
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| ISSN: | 2590-1575 |