Antimicrobial Activity of Cinnamon, Tea Tree, and Thyme Essential Oils Against Pathogenic Bacteria Isolated from Tilapia (<i>Oreochromis</i> spp.) in Aquaculture Farms
Overexploitation has led to a rise in pathogenic bacteria within aquaculture, increasing reliance on antibiotics, and developing microorganism resistance. This situation underscores the need to explore alternatives with a reduced ecological impact. Metabolites derived from essential oils have demons...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/13/2799 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839631691462737920 |
|---|---|
| author | Karen A. Terrazas-Pineda Liliana Alamilla-Beltrán Claudia Ariadna Acero-Ortega Juan Antonio Damas-Espinoza Georgina Calderón-Domínguez Rosalva Mora-Escobedo Vicente Vega-Sánchez Fabián Ricardo Gómez-de Anda |
| author_facet | Karen A. Terrazas-Pineda Liliana Alamilla-Beltrán Claudia Ariadna Acero-Ortega Juan Antonio Damas-Espinoza Georgina Calderón-Domínguez Rosalva Mora-Escobedo Vicente Vega-Sánchez Fabián Ricardo Gómez-de Anda |
| author_sort | Karen A. Terrazas-Pineda |
| collection | DOAJ |
| description | Overexploitation has led to a rise in pathogenic bacteria within aquaculture, increasing reliance on antibiotics, and developing microorganism resistance. This situation underscores the need to explore alternatives with a reduced ecological impact. Metabolites derived from essential oils have demonstrated antimicrobial properties that can inhibit or diminish the activity of various microorganisms. In this study, the antimicrobial efficacy of cinnamon (<i>Cinnamomum zeylanicum)</i>, tea tree (<i>Melaleuca alternifolia</i>), and thyme (<i>Thymus vulgaris</i>) essential oils against pathogenic bacteria (<i>Aeromonas</i>, <i>Pseudomonas</i>, <i>Shewanella</i>, <i>Comamonas</i>, <i>Vibrio</i>, <i>Acinetobacter</i>, and <i>Empedobacter</i>) isolated from tilapia (<i>Oreochromis</i> spp.) brooded in Hidalgo State, Mexico, were investigated. Diffusion tests were conducted using discs infused with 12 different antibiotics and discs infused with essential oils at concentrations of 15, 10, and 5 μL each. Minimal inhibitory concentration tests were performed using a 96-well microplate format. All bacterial strains exhibited multi-resistance to various antibiotics; however, thyme and cinnamon effectively inhibited the tested bacteria at the lowest concentrations, while tea tree oil was the least effective. The findings suggest the potential incorporation of thyme and cinnamon as an alternative prevention to decrease the use of antibiotic treatment. |
| format | Article |
| id | doaj-art-b0b47f8c67be4ec1bacbe5bb7c609171 |
| institution | Matheson Library |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-b0b47f8c67be4ec1bacbe5bb7c6091712025-07-11T14:41:06ZengMDPI AGMolecules1420-30492025-06-013013279910.3390/molecules30132799Antimicrobial Activity of Cinnamon, Tea Tree, and Thyme Essential Oils Against Pathogenic Bacteria Isolated from Tilapia (<i>Oreochromis</i> spp.) in Aquaculture FarmsKaren A. Terrazas-Pineda0Liliana Alamilla-Beltrán1Claudia Ariadna Acero-Ortega2Juan Antonio Damas-Espinoza3Georgina Calderón-Domínguez4Rosalva Mora-Escobedo5Vicente Vega-Sánchez6Fabián Ricardo Gómez-de Anda7Departamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Zacatenco, Av. Wilfrido Massieu 399, Col. Nueva Industrial Vallejo, Alcaldía Gustavo A. Madero, Ciudad de México CP 07738, MexicoDepartamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Zacatenco, Av. Wilfrido Massieu 399, Col. Nueva Industrial Vallejo, Alcaldía Gustavo A. Madero, Ciudad de México CP 07738, MexicoESDAI, Universidad Panamericana, Augusto Rodin 498, Ciudad de México CP 03920, MexicoDepartamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Zacatenco, Av. Wilfrido Massieu 399, Col. Nueva Industrial Vallejo, Alcaldía Gustavo A. Madero, Ciudad de México CP 07738, MexicoDepartamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Zacatenco, Av. Wilfrido Massieu 399, Col. Nueva Industrial Vallejo, Alcaldía Gustavo A. Madero, Ciudad de México CP 07738, MexicoDepartamento de Ingeniería Bioquímica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Zacatenco, Av. Wilfrido Massieu 399, Col. Nueva Industrial Vallejo, Alcaldía Gustavo A. Madero, Ciudad de México CP 07738, MexicoÁrea Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Avenida Universidad Km. 1, Ex hacienda Aquetzalpa, Tulancingo CP 43600, Hidalgo, MexicoÁrea Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Avenida Universidad Km. 1, Ex hacienda Aquetzalpa, Tulancingo CP 43600, Hidalgo, MexicoOverexploitation has led to a rise in pathogenic bacteria within aquaculture, increasing reliance on antibiotics, and developing microorganism resistance. This situation underscores the need to explore alternatives with a reduced ecological impact. Metabolites derived from essential oils have demonstrated antimicrobial properties that can inhibit or diminish the activity of various microorganisms. In this study, the antimicrobial efficacy of cinnamon (<i>Cinnamomum zeylanicum)</i>, tea tree (<i>Melaleuca alternifolia</i>), and thyme (<i>Thymus vulgaris</i>) essential oils against pathogenic bacteria (<i>Aeromonas</i>, <i>Pseudomonas</i>, <i>Shewanella</i>, <i>Comamonas</i>, <i>Vibrio</i>, <i>Acinetobacter</i>, and <i>Empedobacter</i>) isolated from tilapia (<i>Oreochromis</i> spp.) brooded in Hidalgo State, Mexico, were investigated. Diffusion tests were conducted using discs infused with 12 different antibiotics and discs infused with essential oils at concentrations of 15, 10, and 5 μL each. Minimal inhibitory concentration tests were performed using a 96-well microplate format. All bacterial strains exhibited multi-resistance to various antibiotics; however, thyme and cinnamon effectively inhibited the tested bacteria at the lowest concentrations, while tea tree oil was the least effective. The findings suggest the potential incorporation of thyme and cinnamon as an alternative prevention to decrease the use of antibiotic treatment.https://www.mdpi.com/1420-3049/30/13/2799<i>Vibrio</i><i>Aeromonas</i><i>Pseudomonas</i>thymolcinnamaldehydeantibiotic resistance |
| spellingShingle | Karen A. Terrazas-Pineda Liliana Alamilla-Beltrán Claudia Ariadna Acero-Ortega Juan Antonio Damas-Espinoza Georgina Calderón-Domínguez Rosalva Mora-Escobedo Vicente Vega-Sánchez Fabián Ricardo Gómez-de Anda Antimicrobial Activity of Cinnamon, Tea Tree, and Thyme Essential Oils Against Pathogenic Bacteria Isolated from Tilapia (<i>Oreochromis</i> spp.) in Aquaculture Farms Molecules <i>Vibrio</i> <i>Aeromonas</i> <i>Pseudomonas</i> thymol cinnamaldehyde antibiotic resistance |
| title | Antimicrobial Activity of Cinnamon, Tea Tree, and Thyme Essential Oils Against Pathogenic Bacteria Isolated from Tilapia (<i>Oreochromis</i> spp.) in Aquaculture Farms |
| title_full | Antimicrobial Activity of Cinnamon, Tea Tree, and Thyme Essential Oils Against Pathogenic Bacteria Isolated from Tilapia (<i>Oreochromis</i> spp.) in Aquaculture Farms |
| title_fullStr | Antimicrobial Activity of Cinnamon, Tea Tree, and Thyme Essential Oils Against Pathogenic Bacteria Isolated from Tilapia (<i>Oreochromis</i> spp.) in Aquaculture Farms |
| title_full_unstemmed | Antimicrobial Activity of Cinnamon, Tea Tree, and Thyme Essential Oils Against Pathogenic Bacteria Isolated from Tilapia (<i>Oreochromis</i> spp.) in Aquaculture Farms |
| title_short | Antimicrobial Activity of Cinnamon, Tea Tree, and Thyme Essential Oils Against Pathogenic Bacteria Isolated from Tilapia (<i>Oreochromis</i> spp.) in Aquaculture Farms |
| title_sort | antimicrobial activity of cinnamon tea tree and thyme essential oils against pathogenic bacteria isolated from tilapia i oreochromis i spp in aquaculture farms |
| topic | <i>Vibrio</i> <i>Aeromonas</i> <i>Pseudomonas</i> thymol cinnamaldehyde antibiotic resistance |
| url | https://www.mdpi.com/1420-3049/30/13/2799 |
| work_keys_str_mv | AT karenaterrazaspineda antimicrobialactivityofcinnamonteatreeandthymeessentialoilsagainstpathogenicbacteriaisolatedfromtilapiaioreochromisisppinaquaculturefarms AT lilianaalamillabeltran antimicrobialactivityofcinnamonteatreeandthymeessentialoilsagainstpathogenicbacteriaisolatedfromtilapiaioreochromisisppinaquaculturefarms AT claudiaariadnaaceroortega antimicrobialactivityofcinnamonteatreeandthymeessentialoilsagainstpathogenicbacteriaisolatedfromtilapiaioreochromisisppinaquaculturefarms AT juanantoniodamasespinoza antimicrobialactivityofcinnamonteatreeandthymeessentialoilsagainstpathogenicbacteriaisolatedfromtilapiaioreochromisisppinaquaculturefarms AT georginacalderondominguez antimicrobialactivityofcinnamonteatreeandthymeessentialoilsagainstpathogenicbacteriaisolatedfromtilapiaioreochromisisppinaquaculturefarms AT rosalvamoraescobedo antimicrobialactivityofcinnamonteatreeandthymeessentialoilsagainstpathogenicbacteriaisolatedfromtilapiaioreochromisisppinaquaculturefarms AT vicentevegasanchez antimicrobialactivityofcinnamonteatreeandthymeessentialoilsagainstpathogenicbacteriaisolatedfromtilapiaioreochromisisppinaquaculturefarms AT fabianricardogomezdeanda antimicrobialactivityofcinnamonteatreeandthymeessentialoilsagainstpathogenicbacteriaisolatedfromtilapiaioreochromisisppinaquaculturefarms |