Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor Conditions
Mite-pathogenic Akanthomyces attenuatus JEF-147 was liquid-cultured to produce blastospores as an active ingredient of formulations, which is more practical and cost-effective method than currently used solid cultures although stress resistance remains a challenge. In this work, first starch- or glu...
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Taylor & Francis Group
2025-07-01
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| Series: | Mycobiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/12298093.2025.2532235 |
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| author | Gahyeon Song Mi Rong Lee Jae Su Kim |
| author_facet | Gahyeon Song Mi Rong Lee Jae Su Kim |
| author_sort | Gahyeon Song |
| collection | DOAJ |
| description | Mite-pathogenic Akanthomyces attenuatus JEF-147 was liquid-cultured to produce blastospores as an active ingredient of formulations, which is more practical and cost-effective method than currently used solid cultures although stress resistance remains a challenge. In this work, first starch- or glucose-based artificial liquid culture media were used to culture JEF-147 with high miticidal activity against two-spotted spider mites (Tetranychus urticae) in flask conditions. The starch-based medium produced significantly higher numbers of blastospores than the glucose-based medium. JEF-147 blastospores from the glucose-based medium showed faster pathogenesis than those from starch-based medium. This liquid cultured was scaled up in a 2.5-L bioreactor with the glucose-based medium and 1.0 v/v/m of air supply. Production of blastospores was initiated 36 h after inoculation, but active blastospore production was not observed even in 94 h. Alternatively, the glucose-based medium was supplemented with magnesium sulfate at 1% (w/w) in the same bioreactor conditions, and it produced large number of blastospores. This work suggests that JEF-147 blastospores from the glucose-based liquid culture could be used for mite control and the supplement of salt to the medium possibly ensures the production of blastospores in a scale-up conditions. Specific optimization of salt and culture parameters in the scale up conditions and field study would be essential works to be followed. |
| format | Article |
| id | doaj-art-afa25913f86042a3b7099d2e8c20c3c5 |
| institution | Matheson Library |
| issn | 1229-8093 2092-9323 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Taylor & Francis Group |
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| series | Mycobiology |
| spelling | doaj-art-afa25913f86042a3b7099d2e8c20c3c52025-07-30T03:45:17ZengTaylor & Francis GroupMycobiology1229-80932092-93232025-07-0153457258310.1080/12298093.2025.2532235Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor ConditionsGahyeon Song0Mi Rong Lee1Jae Su Kim2Department of Plant Medicine, Jeonbuk National University, Jeonju, Republic of KoreaDepartment of Plant Medicine, Jeonbuk National University, Jeonju, Republic of KoreaDepartment of Plant Medicine, Jeonbuk National University, Jeonju, Republic of KoreaMite-pathogenic Akanthomyces attenuatus JEF-147 was liquid-cultured to produce blastospores as an active ingredient of formulations, which is more practical and cost-effective method than currently used solid cultures although stress resistance remains a challenge. In this work, first starch- or glucose-based artificial liquid culture media were used to culture JEF-147 with high miticidal activity against two-spotted spider mites (Tetranychus urticae) in flask conditions. The starch-based medium produced significantly higher numbers of blastospores than the glucose-based medium. JEF-147 blastospores from the glucose-based medium showed faster pathogenesis than those from starch-based medium. This liquid cultured was scaled up in a 2.5-L bioreactor with the glucose-based medium and 1.0 v/v/m of air supply. Production of blastospores was initiated 36 h after inoculation, but active blastospore production was not observed even in 94 h. Alternatively, the glucose-based medium was supplemented with magnesium sulfate at 1% (w/w) in the same bioreactor conditions, and it produced large number of blastospores. This work suggests that JEF-147 blastospores from the glucose-based liquid culture could be used for mite control and the supplement of salt to the medium possibly ensures the production of blastospores in a scale-up conditions. Specific optimization of salt and culture parameters in the scale up conditions and field study would be essential works to be followed.https://www.tandfonline.com/doi/10.1080/12298093.2025.2532235Akanthomyces attenuatustetranychus urticaeglucoseMgSO4bioreactor |
| spellingShingle | Gahyeon Song Mi Rong Lee Jae Su Kim Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor Conditions Mycobiology Akanthomyces attenuatus tetranychus urticae glucose MgSO4 bioreactor |
| title | Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor Conditions |
| title_full | Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor Conditions |
| title_fullStr | Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor Conditions |
| title_full_unstemmed | Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor Conditions |
| title_short | Production of Mite-Pathogenic Akanthomyces attenuatus JEF-147 Blastospores in Flask and Bioreactor Conditions |
| title_sort | production of mite pathogenic akanthomyces attenuatus jef 147 blastospores in flask and bioreactor conditions |
| topic | Akanthomyces attenuatus tetranychus urticae glucose MgSO4 bioreactor |
| url | https://www.tandfonline.com/doi/10.1080/12298093.2025.2532235 |
| work_keys_str_mv | AT gahyeonsong productionofmitepathogenicakanthomycesattenuatusjef147blastosporesinflaskandbioreactorconditions AT mironglee productionofmitepathogenicakanthomycesattenuatusjef147blastosporesinflaskandbioreactorconditions AT jaesukim productionofmitepathogenicakanthomycesattenuatusjef147blastosporesinflaskandbioreactorconditions |