Characterisation of potential interactions between urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and whey protein isolate
Urea fertilizer augmented with the urease inhibitor (UI) N-(n-butyl)-thiophosphoric triamide (NBPT), is used to stabilize nitrogen and reduce greenhouse gas emissions. Although studies have shown that NBPT does not enter milk via cattle grazing on NBPT-treated fields, questions remain about its pote...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-07-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525006479 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839594084632625152 |
|---|---|
| author | Maria P. Byrne Patrick J. Forrestal Tom F.O' Callaghan Niharika Rahman Aishwarya Ray Martin Danaher Bernard M. Corrigan Chikere G. Nkwonta Karl Richards Farhad Garavand Enda Cummins Sean A. Hogan |
| author_facet | Maria P. Byrne Patrick J. Forrestal Tom F.O' Callaghan Niharika Rahman Aishwarya Ray Martin Danaher Bernard M. Corrigan Chikere G. Nkwonta Karl Richards Farhad Garavand Enda Cummins Sean A. Hogan |
| author_sort | Maria P. Byrne |
| collection | DOAJ |
| description | Urea fertilizer augmented with the urease inhibitor (UI) N-(n-butyl)-thiophosphoric triamide (NBPT), is used to stabilize nitrogen and reduce greenhouse gas emissions. Although studies have shown that NBPT does not enter milk via cattle grazing on NBPT-treated fields, questions remain about its potential to impact milk components were it inadvertently to enter the dairy supply chain. The aim of this study was to examine possible effects of NBPT on the chemistry and functionality of whey protein isolate (WPI). NBPT raised the peak denaturation temperature of WPI (p < 0.05) and resulted in increased hydrogen bonding and β-sheet formation. NBPT reduced the viscosity of WPI and caused faster gelation. NBPT had a competitive adsorption advantage at the air-water interface (p < 0.05) compared to WPI. Microscopy imaging of WPI-NBPT gels revealed increased protein aggregation. Overall, NBPT modified the structure and functionality of WPI, primarily through non-covalent interactions. |
| format | Article |
| id | doaj-art-af75af06b2694e84b5591f5c90a35c2b |
| institution | Matheson Library |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-af75af06b2694e84b5591f5c90a35c2b2025-08-03T04:43:03ZengElsevierFood Chemistry: X2590-15752025-07-0129102800Characterisation of potential interactions between urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and whey protein isolateMaria P. Byrne0Patrick J. Forrestal1Tom F.O' Callaghan2Niharika Rahman3Aishwarya Ray4Martin Danaher5Bernard M. Corrigan6Chikere G. Nkwonta7Karl Richards8Farhad Garavand9Enda Cummins10Sean A. Hogan11Food Chemistry and Technology Department, Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, P61 C996, Cork, Ireland; School of Biosystems and Food Engineering, Agriculture and Food Science, University College Dublin, Belfield, 4, Dublin, IrelandTeagasc, Johnstown Castle, Y35 Y521 Co. Wexford, IrelandSchool of Food and Nutritional Sciences, University College Cork, Gaol Walk, T12 YN60, Cork, IrelandTeagasc, Johnstown Castle, Y35 Y521 Co. Wexford, IrelandSchool of Biosystems and Food Engineering, Agriculture and Food Science, University College Dublin, Belfield, 4, Dublin, IrelandFood Safety Department, Ashtown Food Research Centre, Teagasc, Ashtown, 15, Dublin, IrelandFood Chemistry and Technology Department, Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, P61 C996, Cork, IrelandDepartment of Biosciences, Durham University, United KingdomTeagasc, Johnstown Castle, Y35 Y521 Co. Wexford, IrelandDepartment of Biological Sciences, University of Limerick, V94 T9PX, Limerick, IrelandSchool of Biosystems and Food Engineering, Agriculture and Food Science, University College Dublin, Belfield, 4, Dublin, IrelandFood Chemistry and Technology Department, Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, P61 C996, Cork, Ireland; Corresponding author.Urea fertilizer augmented with the urease inhibitor (UI) N-(n-butyl)-thiophosphoric triamide (NBPT), is used to stabilize nitrogen and reduce greenhouse gas emissions. Although studies have shown that NBPT does not enter milk via cattle grazing on NBPT-treated fields, questions remain about its potential to impact milk components were it inadvertently to enter the dairy supply chain. The aim of this study was to examine possible effects of NBPT on the chemistry and functionality of whey protein isolate (WPI). NBPT raised the peak denaturation temperature of WPI (p < 0.05) and resulted in increased hydrogen bonding and β-sheet formation. NBPT reduced the viscosity of WPI and caused faster gelation. NBPT had a competitive adsorption advantage at the air-water interface (p < 0.05) compared to WPI. Microscopy imaging of WPI-NBPT gels revealed increased protein aggregation. Overall, NBPT modified the structure and functionality of WPI, primarily through non-covalent interactions.http://www.sciencedirect.com/science/article/pii/S2590157525006479Urease inhibitorNBPTSurfactantSustainable ureaWhey protein isolateRheology |
| spellingShingle | Maria P. Byrne Patrick J. Forrestal Tom F.O' Callaghan Niharika Rahman Aishwarya Ray Martin Danaher Bernard M. Corrigan Chikere G. Nkwonta Karl Richards Farhad Garavand Enda Cummins Sean A. Hogan Characterisation of potential interactions between urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and whey protein isolate Food Chemistry: X Urease inhibitor NBPT Surfactant Sustainable urea Whey protein isolate Rheology |
| title | Characterisation of potential interactions between urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and whey protein isolate |
| title_full | Characterisation of potential interactions between urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and whey protein isolate |
| title_fullStr | Characterisation of potential interactions between urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and whey protein isolate |
| title_full_unstemmed | Characterisation of potential interactions between urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and whey protein isolate |
| title_short | Characterisation of potential interactions between urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and whey protein isolate |
| title_sort | characterisation of potential interactions between urease inhibitor n n butyl thiophosphoric triamide nbpt and whey protein isolate |
| topic | Urease inhibitor NBPT Surfactant Sustainable urea Whey protein isolate Rheology |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525006479 |
| work_keys_str_mv | AT mariapbyrne characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT patrickjforrestal characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT tomfocallaghan characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT niharikarahman characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT aishwaryaray characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT martindanaher characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT bernardmcorrigan characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT chikeregnkwonta characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT karlrichards characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT farhadgaravand characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT endacummins characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate AT seanahogan characterisationofpotentialinteractionsbetweenureaseinhibitornnbutylthiophosphorictriamidenbptandwheyproteinisolate |