Activation of X-Succinate Synthases for Fumarate Hydroalkylation Using an In Vitro Activation Method

X-succinate synthase enzymes (XSSs) are a class of glycyl radical enzymes (GREs) that play a pivotal role in microbial anaerobic hydrocarbon degradation. They catalyze the addition of hydrocarbons to fumarate using a protein-based glycyl radical, which must first be installed by a radical S-adenosyl...

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Bibliographic Details
Main Authors: Anshika Vats, Shukurah Anas, Ankush Chakraborty, Jian Liu, Jimyung Ryu, Sara Mann, Mary Andorfer
Format: Article
Language:English
Published: Bio-protocol LLC 2025-06-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5357&type=0
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