A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources

Global protein demand is rising as populations grow and consumers seek sustainable and nutritious alternatives. Plant-based proteins are favored for their consumer acceptance, lower environmental impact, and health benefits. However, the main challenge in extracting high-quality and functional plant...

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Main Authors: Paul Ndubuisi Anyiam, Suphat Phongthai, Passakorn Kingwascharapong, Jaksuma Pongsetkul, Wanli Zhang, Young Hoon Jung, Saroat Rawdkuen
Format: Article
Language:English
Published: Elsevier 2025-10-01
Series:NFS Journal
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352364625000288
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author Paul Ndubuisi Anyiam
Suphat Phongthai
Passakorn Kingwascharapong
Jaksuma Pongsetkul
Wanli Zhang
Young Hoon Jung
Saroat Rawdkuen
author_facet Paul Ndubuisi Anyiam
Suphat Phongthai
Passakorn Kingwascharapong
Jaksuma Pongsetkul
Wanli Zhang
Young Hoon Jung
Saroat Rawdkuen
author_sort Paul Ndubuisi Anyiam
collection DOAJ
description Global protein demand is rising as populations grow and consumers seek sustainable and nutritious alternatives. Plant-based proteins are favored for their consumer acceptance, lower environmental impact, and health benefits. However, the main challenge in extracting high-quality and functional plant proteins for food applications is to select the most suitable extraction method. Conventional protein extraction methods (acid-alkaline and salt extraction) have several drawbacks. Novel-assisted extraction techniques such as enzyme-assisted, ultrasound-assisted, microwave-assisted, high-pressure-assisted, and pulse electric field-assisted extractions, have proven effective in enhancing the yield of high-quality protein. This review examines recent data on novel protein extraction technologies from six promising plant protein sources under optimized conditions, focusing on their yield, protein quality, and functional properties, as well as mechanisms, drawbacks and future perspectives compared to conventional techniques. The final section explores recent trends in enriching foods with protein isolated from some potential plant sources, highlighting their ability to improve food quality and tackle protein deficiencies. The available data indicate that under optimized conditions, cell-disruptive techniques have the potential to improve protein recovery, and functional properties from plant sources, while minimizing environmental pollution. Given the recent surge in interest in alternative plant-based proteins, this review will offer insights and references for future research and development in plant-based protein processing.
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spelling doaj-art-aeee39e9fc5a4f9c9d3b45e51cca3e792025-07-02T04:50:26ZengElsevierNFS Journal2352-36462025-10-0140100239A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sourcesPaul Ndubuisi Anyiam0Suphat Phongthai1Passakorn Kingwascharapong2Jaksuma Pongsetkul3Wanli Zhang4Young Hoon Jung5Saroat Rawdkuen6Innovative Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand; Department of Biochemistry, College of Natural Science, Michael Okpara University of Agriculture, Umudike, Umuahia, P.M.B 7262, Abia State, NigeriaDivision of Food Science and Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Fishery Products, Faculty of Fisheries, Kasetsart University, 10900, ThailandSchool of Animal Technology and Innovation, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandCollege of Food Science and Technology, Hainan University, Haikou 570228, ChinaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of KoreaInnovative Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand; Unit of Innovative Food Packaging and Biomaterials, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand; Corresponding author at: School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand.Global protein demand is rising as populations grow and consumers seek sustainable and nutritious alternatives. Plant-based proteins are favored for their consumer acceptance, lower environmental impact, and health benefits. However, the main challenge in extracting high-quality and functional plant proteins for food applications is to select the most suitable extraction method. Conventional protein extraction methods (acid-alkaline and salt extraction) have several drawbacks. Novel-assisted extraction techniques such as enzyme-assisted, ultrasound-assisted, microwave-assisted, high-pressure-assisted, and pulse electric field-assisted extractions, have proven effective in enhancing the yield of high-quality protein. This review examines recent data on novel protein extraction technologies from six promising plant protein sources under optimized conditions, focusing on their yield, protein quality, and functional properties, as well as mechanisms, drawbacks and future perspectives compared to conventional techniques. The final section explores recent trends in enriching foods with protein isolated from some potential plant sources, highlighting their ability to improve food quality and tackle protein deficiencies. The available data indicate that under optimized conditions, cell-disruptive techniques have the potential to improve protein recovery, and functional properties from plant sources, while minimizing environmental pollution. Given the recent surge in interest in alternative plant-based proteins, this review will offer insights and references for future research and development in plant-based protein processing.http://www.sciencedirect.com/science/article/pii/S2352364625000288Plant-based proteinsAssisted-extractionEmerging techniquesAlternative proteinFood enrichment
spellingShingle Paul Ndubuisi Anyiam
Suphat Phongthai
Passakorn Kingwascharapong
Jaksuma Pongsetkul
Wanli Zhang
Young Hoon Jung
Saroat Rawdkuen
A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources
NFS Journal
Plant-based proteins
Assisted-extraction
Emerging techniques
Alternative protein
Food enrichment
title A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources
title_full A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources
title_fullStr A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources
title_full_unstemmed A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources
title_short A comparative review on novel-assisted extraction techniques for retrieving protein from some potential plant sources
title_sort comparative review on novel assisted extraction techniques for retrieving protein from some potential plant sources
topic Plant-based proteins
Assisted-extraction
Emerging techniques
Alternative protein
Food enrichment
url http://www.sciencedirect.com/science/article/pii/S2352364625000288
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