Protein Coding Low‐Copy rpb2 and ef1‐α Regions Are Viable Fungal Metabarcoding DNA Markers Which Can Supplement ITS for Better Accuracy

Protein Coding Low‐Copy rpb2 and ef1‐α Regions Are Viable Fungal Metabarcoding DNA Markers Which Can Supplement ITS for Better Accuracy

ABSTRACT The nuclear ribosomal DNA Internal Transcribed Spacer (ITS) region is used as a universal fungal barcode marker, but often lacks a significant DNA barcoding gap between sister taxa. Here we tested the reliability of protein coding low‐copy genes as alternative barcode markers. Mock communit...

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Bibliographic Details
Main Authors: Vasilii Shapkin, Miroslav Caboň, Miroslav Kolařík, Katarína Adamčíková, Petr Baldrian, Tereza Michalová, Tomáš Větrovský, Slavomír Adamčík
Format: Article
Language:English
Published: Wiley 2025-04-01
Series:Ecology and Evolution
Subjects:
amplicon abundance
chimera
sympatric species
threshold
Online Access:https://doi.org/10.1002/ece3.71352
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