Development of an ELISPOT assay for numerating IFN-γ-secreting T cells in chicken using novel monoclonal antibodies

Development of an ELISPOT assay for numerating IFN-γ-secreting T cells in chicken using novel monoclonal antibodies

Cellular immune responses play critical roles in the control of pathogenic infection. The measurement of antigen-specific IFN-γ-secreting T cells via an enzyme-linked immunospot assay (ELISPOT) is a valuable method for the evaluation of cellular immune responses. However, in chicken, few of monoclon...

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Bibliographic Details
Main Authors: Zhou Zhou, Ji Wu, Huining Zhang, Xinjie Yuan, Ye Meng, Lina Chen, Yi Yang, Xiaoli Hao, Shaobin Shang
Format: Article
Language:English
Published: Elsevier 2025-10-01
Series:Poultry Science
Subjects:
Chicken interferon-γ
Monoclonal antibody
ELISPOT
IFN-γ-secreting T cells
Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125007679
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