Effects of Orally Delivered Double-Stranded RNA of <i>Trehalose-6-Phosphate Synthase</i> on the Population of <i>Frankliniella occidentalis</i>
The invasive pest <i>Frankliniella occidentalis</i> (western flower thrip, WFT) severely impacts agricultural production. RNA interference (RNAi) has emerged as a viable alternative to chemical control methods. Trehalose-6-phosphate synthase (TPS) is an important enzyme for the synthesis...
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MDPI AG
2025-06-01
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| Series: | Insects |
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| author | Tao Lin Xiaoyu Chen Ying Chen Ting Chen Xueyi Liang Hui Wei Guang Yang |
| author_facet | Tao Lin Xiaoyu Chen Ying Chen Ting Chen Xueyi Liang Hui Wei Guang Yang |
| author_sort | Tao Lin |
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| description | The invasive pest <i>Frankliniella occidentalis</i> (western flower thrip, WFT) severely impacts agricultural production. RNA interference (RNAi) has emerged as a viable alternative to chemical control methods. Trehalose-6-phosphate synthase (TPS) is an important enzyme for the synthesis of trehalose in insects. The <i>TPS</i> gene is a potential target for RNAi-based pest control; however, its efficacy against WFTs remains unclear. Feeding with the synthesized dsFoTPS to WFT significantly increased the mortalities of first- and second-instar nymphs and adults and significantly decreased the number of eggs laid by adults within 72 h; the expression of <i>FoTPS</i> was significantly down-regulated in 24, 48, and 72 h. Ingestion of WFTs on <i>Escherichia coli</i> expressing dsFoTPS led to a marked increase in the total pre-reproductive period; reductions in survival rate, adult longevity, oviposition days, fecundity, peak reproduction value, the intrinsic rate of increase, the finite rate of increase, and the net reproductive rate; and an extension of the mean generation time. <i>TPS</i> gene expression was significantly down-regulated on days 7 and 28. A population simulation of WFTs fed with <i>E. coli</i> expressing dsFoTPS indicated that the population suppression was reduced to 1/34 of the control in 100 d. Oral delivery of <i>E. coli</i> expressing dsFoTPS effectively inhibited the survival, fecundity, and population growth of WFTs, offering a novel approach and rationale for the prevention and control of WFTs. |
| format | Article |
| id | doaj-art-add75fbe2bb24d96bcea4b09c0f9e45d |
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| issn | 2075-4450 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj-art-add75fbe2bb24d96bcea4b09c0f9e45d2025-06-25T13:58:28ZengMDPI AGInsects2075-44502025-06-0116661410.3390/insects16060614Effects of Orally Delivered Double-Stranded RNA of <i>Trehalose-6-Phosphate Synthase</i> on the Population of <i>Frankliniella occidentalis</i>Tao Lin0Xiaoyu Chen1Ying Chen2Ting Chen3Xueyi Liang4Hui Wei5Guang Yang6State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Engineering Research Center for Green Pest Management, Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaState Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFujian Engineering Research Center for Green Pest Management, Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaState Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe invasive pest <i>Frankliniella occidentalis</i> (western flower thrip, WFT) severely impacts agricultural production. RNA interference (RNAi) has emerged as a viable alternative to chemical control methods. Trehalose-6-phosphate synthase (TPS) is an important enzyme for the synthesis of trehalose in insects. The <i>TPS</i> gene is a potential target for RNAi-based pest control; however, its efficacy against WFTs remains unclear. Feeding with the synthesized dsFoTPS to WFT significantly increased the mortalities of first- and second-instar nymphs and adults and significantly decreased the number of eggs laid by adults within 72 h; the expression of <i>FoTPS</i> was significantly down-regulated in 24, 48, and 72 h. Ingestion of WFTs on <i>Escherichia coli</i> expressing dsFoTPS led to a marked increase in the total pre-reproductive period; reductions in survival rate, adult longevity, oviposition days, fecundity, peak reproduction value, the intrinsic rate of increase, the finite rate of increase, and the net reproductive rate; and an extension of the mean generation time. <i>TPS</i> gene expression was significantly down-regulated on days 7 and 28. A population simulation of WFTs fed with <i>E. coli</i> expressing dsFoTPS indicated that the population suppression was reduced to 1/34 of the control in 100 d. Oral delivery of <i>E. coli</i> expressing dsFoTPS effectively inhibited the survival, fecundity, and population growth of WFTs, offering a novel approach and rationale for the prevention and control of WFTs.https://www.mdpi.com/2075-4450/16/6/614feedingtrehalose-6-phosphate synthaselife tablepopulation suppressionbacterially expressed dsRNAwestern flower thrip |
| spellingShingle | Tao Lin Xiaoyu Chen Ying Chen Ting Chen Xueyi Liang Hui Wei Guang Yang Effects of Orally Delivered Double-Stranded RNA of <i>Trehalose-6-Phosphate Synthase</i> on the Population of <i>Frankliniella occidentalis</i> Insects feeding trehalose-6-phosphate synthase life table population suppression bacterially expressed dsRNA western flower thrip |
| title | Effects of Orally Delivered Double-Stranded RNA of <i>Trehalose-6-Phosphate Synthase</i> on the Population of <i>Frankliniella occidentalis</i> |
| title_full | Effects of Orally Delivered Double-Stranded RNA of <i>Trehalose-6-Phosphate Synthase</i> on the Population of <i>Frankliniella occidentalis</i> |
| title_fullStr | Effects of Orally Delivered Double-Stranded RNA of <i>Trehalose-6-Phosphate Synthase</i> on the Population of <i>Frankliniella occidentalis</i> |
| title_full_unstemmed | Effects of Orally Delivered Double-Stranded RNA of <i>Trehalose-6-Phosphate Synthase</i> on the Population of <i>Frankliniella occidentalis</i> |
| title_short | Effects of Orally Delivered Double-Stranded RNA of <i>Trehalose-6-Phosphate Synthase</i> on the Population of <i>Frankliniella occidentalis</i> |
| title_sort | effects of orally delivered double stranded rna of i trehalose 6 phosphate synthase i on the population of i frankliniella occidentalis i |
| topic | feeding trehalose-6-phosphate synthase life table population suppression bacterially expressed dsRNA western flower thrip |
| url | https://www.mdpi.com/2075-4450/16/6/614 |
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