Super-resolution microscopy reveals glioma cell footprints and exosome deposits
Gliomas are aggressive brain tumors whose infiltrative growth is mediated by intercellular crosstalk. Exosomes, small extracellular vesicles, play a key role in cell–cell communication but are difficult to visualize using conventional microscopy. Performing immunostaining for CD63, a known exosome m...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Cell Adhesion & Migration |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19336918.2025.2534759 |
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| author | Stefania Petrini Frédéric Eghiaian Valentina Apollonio Giulia Pericoli Maria Vinci |
| author_facet | Stefania Petrini Frédéric Eghiaian Valentina Apollonio Giulia Pericoli Maria Vinci |
| author_sort | Stefania Petrini |
| collection | DOAJ |
| description | Gliomas are aggressive brain tumors whose infiltrative growth is mediated by intercellular crosstalk. Exosomes, small extracellular vesicles, play a key role in cell–cell communication but are difficult to visualize using conventional microscopy. Performing immunostaining for CD63, a known exosome marker, and using STED microscopy, we demonstrate exosome secretion in primary glioma cells. Applying mathematical deconvolution, we enhance the contrast and resolution for in-depth analysis of STED images. We identify CD63-positive cellular footprints and exosome deposits in the extracellular space. Quantitative analysis shows CD63-positive exosomes ranging 36.55-157.06 nm in size. CD63/actin co-staining demonstrates different actin polymerization states associated with exosomes. In conclusion, STED microscopy coupled with immunostaining allows exosome primary characterization at the single-vesicle level in the cellular spatial context. |
| format | Article |
| id | doaj-art-aad1b105a91544a38ab7d1f2fbb7d071 |
| institution | Matheson Library |
| issn | 1933-6918 1933-6926 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj-art-aad1b105a91544a38ab7d1f2fbb7d0712025-07-24T08:41:28ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262025-12-0119110.1080/19336918.2025.2534759Super-resolution microscopy reveals glioma cell footprints and exosome depositsStefania Petrini0Frédéric Eghiaian1Valentina Apollonio2Giulia Pericoli3Maria Vinci4Confocal Microscopy Core Facility, Research Center, Bambino Gesù Children’s Hospital, IRCCS, Rome, ItalyAbberior Instruments GmbH, Göttingen, GermanyConfocal Microscopy Core Facility, Research Center, Bambino Gesù Children’s Hospital, IRCCS, Rome, ItalyOnco-Haematology and Pharmaceutical GMP Facility Research Area, Bambino Gesù Children’s Hospital, IRCCS, Rome, ItalyOnco-Haematology and Pharmaceutical GMP Facility Research Area, Bambino Gesù Children’s Hospital, IRCCS, Rome, ItalyGliomas are aggressive brain tumors whose infiltrative growth is mediated by intercellular crosstalk. Exosomes, small extracellular vesicles, play a key role in cell–cell communication but are difficult to visualize using conventional microscopy. Performing immunostaining for CD63, a known exosome marker, and using STED microscopy, we demonstrate exosome secretion in primary glioma cells. Applying mathematical deconvolution, we enhance the contrast and resolution for in-depth analysis of STED images. We identify CD63-positive cellular footprints and exosome deposits in the extracellular space. Quantitative analysis shows CD63-positive exosomes ranging 36.55-157.06 nm in size. CD63/actin co-staining demonstrates different actin polymerization states associated with exosomes. In conclusion, STED microscopy coupled with immunostaining allows exosome primary characterization at the single-vesicle level in the cellular spatial context.https://www.tandfonline.com/doi/10.1080/19336918.2025.2534759CD63exosomesgliomamicroscopySTED |
| spellingShingle | Stefania Petrini Frédéric Eghiaian Valentina Apollonio Giulia Pericoli Maria Vinci Super-resolution microscopy reveals glioma cell footprints and exosome deposits Cell Adhesion & Migration CD63 exosomes glioma microscopy STED |
| title | Super-resolution microscopy reveals glioma cell footprints and exosome deposits |
| title_full | Super-resolution microscopy reveals glioma cell footprints and exosome deposits |
| title_fullStr | Super-resolution microscopy reveals glioma cell footprints and exosome deposits |
| title_full_unstemmed | Super-resolution microscopy reveals glioma cell footprints and exosome deposits |
| title_short | Super-resolution microscopy reveals glioma cell footprints and exosome deposits |
| title_sort | super resolution microscopy reveals glioma cell footprints and exosome deposits |
| topic | CD63 exosomes glioma microscopy STED |
| url | https://www.tandfonline.com/doi/10.1080/19336918.2025.2534759 |
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