Neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharide
Aim. To study the expression of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (Iba1) in the cerebral cingulate and retrosplenial cortex of mice on Day 5 after intraperitoneal (i.p.) administration of bacterial lipopolysaccharide (LPS) at the dose with no nervo...
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Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
2024-11-01
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| Series: | Сеченовский вестник |
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| Online Access: | https://www.sechenovmedj.com/jour/article/view/1143 |
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| author | A. A. Venediktov K. S. Pokidova D. M. Oganesyan V. V. Goloborshcheva G. A. Piavchenko S. L. Kuznetsov |
| author_facet | A. A. Venediktov K. S. Pokidova D. M. Oganesyan V. V. Goloborshcheva G. A. Piavchenko S. L. Kuznetsov |
| author_sort | A. A. Venediktov |
| collection | DOAJ |
| description | Aim. To study the expression of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (Iba1) in the cerebral cingulate and retrosplenial cortex of mice on Day 5 after intraperitoneal (i.p.) administration of bacterial lipopolysaccharide (LPS) at the dose with no nervous tissue inflammation provoked.Materials and methods. The work was performed on 10 female C57BL/6 mice aged 90 ± 3 days. At the same time for 4 days, animals of group 1 were intraperitoneally injected with saline (0.9% NaCl), and animals of group 2 were injected with E. coli LPS endotoxin at a dose of 1 mg/kg/day. On the fifth day, the mice were withdrawn from the experiment by decapitation with xylazine/tiletamine-zoletil premedication, after which histological preparations of the cingulate and retrosplenial cortex were made, stained with antibodies to GFAP and Iba1, and the number of: (1) GFAP-positive cells of cytoplasmic areas, (2) cells with a positive reaction of antibodies to Iba1 were counted using QuPath software. Groups were compared using Mann-Whitney U-test.Results. The number of GFAP-positive cells after LPS administration in Group 2 was significantly higher than in Group 1, exactly 22.5 (8.0; 32.0) vs 9.0 (4.3; 17.0), respectively, p = 0.0038. The number of Iba1-positive portions of cytoplasm also was significantly higher in Group 2, namely 207,5 (154,8; 295,8) vs 128 (89,3; 165,5), respectively, p = 0,014. Both groups showed neither signs of inflammation, excessive blood supply nor hemorrhages, as well as no perivascular edema or leukocytic migration.Conclusion. LPS, administered i.p. to mice at a dose of 1 mg/kg/day for 4 days, allows assessment of changes in glia of CNS in damage with no signs of inflammation there. In cerebral cingulate and retrosplenial cortex, the number of astrocytes with a positive reaction of antibodies to GFAP increases, as well as number macrophages with the expression of the Iba1 protein. |
| format | Article |
| id | doaj-art-aa9013eeb71e4d2e8352c9ee8c81a63e |
| institution | Matheson Library |
| issn | 2218-7332 2658-3348 |
| language | Russian |
| publishDate | 2024-11-01 |
| publisher | Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University) |
| record_format | Article |
| series | Сеченовский вестник |
| spelling | doaj-art-aa9013eeb71e4d2e8352c9ee8c81a63e2025-08-03T13:30:19ZrusFederal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)Сеченовский вестник2218-73322658-33482024-11-01153485710.47093/2218-7332.2024.15.3.48-57605Neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharideA. A. Venediktov0K. S. Pokidova1D. M. Oganesyan2V. V. Goloborshcheva3G. A. Piavchenko4S. L. Kuznetsov5Sechenov First Moscow State Medical University (Sechenov University)Sechenov First Moscow State Medical University (Sechenov University)Sechenov First Moscow State Medical University (Sechenov University)Institute of General Pathology and PathophysiologySechenov First Moscow State Medical University (Sechenov University)Sechenov First Moscow State Medical University (Sechenov University)Aim. To study the expression of glial fibrillary acidic protein (GFAP) and ionized calcium-binding adapter molecule 1 (Iba1) in the cerebral cingulate and retrosplenial cortex of mice on Day 5 after intraperitoneal (i.p.) administration of bacterial lipopolysaccharide (LPS) at the dose with no nervous tissue inflammation provoked.Materials and methods. The work was performed on 10 female C57BL/6 mice aged 90 ± 3 days. At the same time for 4 days, animals of group 1 were intraperitoneally injected with saline (0.9% NaCl), and animals of group 2 were injected with E. coli LPS endotoxin at a dose of 1 mg/kg/day. On the fifth day, the mice were withdrawn from the experiment by decapitation with xylazine/tiletamine-zoletil premedication, after which histological preparations of the cingulate and retrosplenial cortex were made, stained with antibodies to GFAP and Iba1, and the number of: (1) GFAP-positive cells of cytoplasmic areas, (2) cells with a positive reaction of antibodies to Iba1 were counted using QuPath software. Groups were compared using Mann-Whitney U-test.Results. The number of GFAP-positive cells after LPS administration in Group 2 was significantly higher than in Group 1, exactly 22.5 (8.0; 32.0) vs 9.0 (4.3; 17.0), respectively, p = 0.0038. The number of Iba1-positive portions of cytoplasm also was significantly higher in Group 2, namely 207,5 (154,8; 295,8) vs 128 (89,3; 165,5), respectively, p = 0,014. Both groups showed neither signs of inflammation, excessive blood supply nor hemorrhages, as well as no perivascular edema or leukocytic migration.Conclusion. LPS, administered i.p. to mice at a dose of 1 mg/kg/day for 4 days, allows assessment of changes in glia of CNS in damage with no signs of inflammation there. In cerebral cingulate and retrosplenial cortex, the number of astrocytes with a positive reaction of antibodies to GFAP increases, as well as number macrophages with the expression of the Iba1 protein.https://www.sechenovmedj.com/jour/article/view/1143nervous tissueastrocytesmicroglialipopolysaccharideglial fibrillary acidic proteinionized calcium-binding adapter molecule 1 |
| spellingShingle | A. A. Venediktov K. S. Pokidova D. M. Oganesyan V. V. Goloborshcheva G. A. Piavchenko S. L. Kuznetsov Neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharide Сеченовский вестник nervous tissue astrocytes microglia lipopolysaccharide glial fibrillary acidic protein ionized calcium-binding adapter molecule 1 |
| title | Neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharide |
| title_full | Neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharide |
| title_fullStr | Neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharide |
| title_full_unstemmed | Neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharide |
| title_short | Neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharide |
| title_sort | neuroglia markers expression in the cingulate and retrosplenial cortex of mice after nonseptic dose of lipopolysaccharide |
| topic | nervous tissue astrocytes microglia lipopolysaccharide glial fibrillary acidic protein ionized calcium-binding adapter molecule 1 |
| url | https://www.sechenovmedj.com/jour/article/view/1143 |
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