Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formation
In this research, an easy, acurate and rapid spectrophotometric method for determination of sulphite and thiamine hydrochloride is described. This method used proton transfer reaction between sulphite ion with alizarine at (pH 6.73) to develop red colour adduct that bears maximum absorption at 524...
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Tikrit University
2019-03-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/333 |
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| author | Bassima A. Abed Al-Hadi |
| author_facet | Bassima A. Abed Al-Hadi |
| author_sort | Bassima A. Abed Al-Hadi |
| collection | DOAJ |
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In this research, an easy, acurate and rapid spectrophotometric method for determination of sulphite and thiamine hydrochloride is described. This method used proton transfer reaction between sulphite ion with alizarine at (pH 6.73) to develop red colour adduct that bears maximum absorption at 524 nm. This method was developed to determination of thiamine hydrochloride by bleaching the red colour (alizarine-sulphite product) at the optimum conditions established for the determination of sulphite. Beer’s law is obeyed in the concentration range (10-1000) μg/25ml (0.4-40) of sulphite and 10-1500, μg/25ml (0.4-60) for thiamine hydrochloride at the selected wavelength, the molar absorbtivity 2.6×104 l.mol-1.cm-1 for SO32- and 3.37×104 l.mol-1.cm-1 for thiamine hydrochloride, Sandell's index for sensitivity 0.00307 μg .cm-2 for sulphite and 0.0100 μg .cm-2 for B1, the precision (RSD) of the method is ±0.23- ± 0.51% sulphite and ±0.15- ±0.25 thiamine hydrochloride. Good recoveries of sulphite and thiamine hydrochloride from water samples and pharmaceutical formation are achieved using the proposed method.
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| format | Article |
| id | doaj-art-a9eaf9acbfd94328aef90a342a8c2d0e |
| institution | Matheson Library |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-a9eaf9acbfd94328aef90a342a8c2d0e2025-07-14T01:50:59ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262019-03-0124110.25130/tjps.v24i1.333Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formationBassima A. Abed Al-Hadi In this research, an easy, acurate and rapid spectrophotometric method for determination of sulphite and thiamine hydrochloride is described. This method used proton transfer reaction between sulphite ion with alizarine at (pH 6.73) to develop red colour adduct that bears maximum absorption at 524 nm. This method was developed to determination of thiamine hydrochloride by bleaching the red colour (alizarine-sulphite product) at the optimum conditions established for the determination of sulphite. Beer’s law is obeyed in the concentration range (10-1000) μg/25ml (0.4-40) of sulphite and 10-1500, μg/25ml (0.4-60) for thiamine hydrochloride at the selected wavelength, the molar absorbtivity 2.6×104 l.mol-1.cm-1 for SO32- and 3.37×104 l.mol-1.cm-1 for thiamine hydrochloride, Sandell's index for sensitivity 0.00307 μg .cm-2 for sulphite and 0.0100 μg .cm-2 for B1, the precision (RSD) of the method is ±0.23- ± 0.51% sulphite and ±0.15- ±0.25 thiamine hydrochloride. Good recoveries of sulphite and thiamine hydrochloride from water samples and pharmaceutical formation are achieved using the proposed method. https://tjpsj.org/index.php/tjps/article/view/333SulphiteThiamine hydrochlorideAlizarinproton transfer reaction |
| spellingShingle | Bassima A. Abed Al-Hadi Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formation Tikrit Journal of Pure Science Sulphite Thiamine hydrochloride Alizarin proton transfer reaction |
| title | Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formation |
| title_full | Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formation |
| title_fullStr | Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formation |
| title_full_unstemmed | Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formation |
| title_short | Spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction-application to water sample and pharmaceutical formation |
| title_sort | spectrophotometric determination of sulphite and thiamin hydrochloride using proton transfer reaction application to water sample and pharmaceutical formation |
| topic | Sulphite Thiamine hydrochloride Alizarin proton transfer reaction |
| url | https://tjpsj.org/index.php/tjps/article/view/333 |
| work_keys_str_mv | AT bassimaaabedalhadi spectrophotometricdeterminationofsulphiteandthiaminhydrochlorideusingprotontransferreactionapplicationtowatersampleandpharmaceuticalformation |