Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of praziquantel and pyrantel pamoate in tablet dosage forms of TOTAL. HPLC separation wascarried out by reversed phase chromatography Betasil C18 (250 mm x 4,6mm i.d.; 5 5m particle size...
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| Format: | Article |
| Language: | English |
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Asociaţia Naţională a Fabricanţilor de Produse de Uz Veterinar
2011-06-01
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| Series: | Medicamentul Veterinar |
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| Online Access: | http://www.veterinarypharmacon.com/docs/944-2011-ART4.%20eng.pdf |
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| _version_ | 1839598661543133184 |
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| author | Elena Gabriela Oltean |
| author_facet | Elena Gabriela Oltean |
| author_sort | Elena Gabriela Oltean |
| collection | DOAJ |
| description | An isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of praziquantel and pyrantel pamoate in tablet dosage forms of TOTAL. HPLC separation wascarried out by reversed phase chromatography Betasil C18 (250 mm x 4,6mm i.d.; 5 5m particle size), held at 25°C respective Kromasil 60-5SIL. (250 mm x 4,6 mm i.d.; 5 5m particle size), held at 25°C. The mobile phase consisted of acetonitrile/distilled water (60/40v/v), run at flow rate of 1 mL/min and with UV detection at 210nm, respective acetonitrile/ 0,1% phosphoric acid aq. (60/40 v/v), run at flow rate of 1 mL/ min and with UV detection at 240 nm. Method validation investigated parameters such as linearity (r2=0,9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of TOTAL tablets. |
| format | Article |
| id | doaj-art-a9346f6b38ef47de9d15d6a8123b6027 |
| institution | Matheson Library |
| issn | 1843-9527 2069-2463 |
| language | English |
| publishDate | 2011-06-01 |
| publisher | Asociaţia Naţională a Fabricanţilor de Produse de Uz Veterinar |
| record_format | Article |
| series | Medicamentul Veterinar |
| spelling | doaj-art-a9346f6b38ef47de9d15d6a8123b60272025-08-02T16:12:52ZengAsociaţia Naţională a Fabricanţilor de Produse de Uz VeterinarMedicamentul Veterinar1843-95272069-24632011-06-01516467Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoateElena Gabriela OlteanAn isocratic high-performance liquid chromatography (HPLC) procedure was developed for quantitative determination of praziquantel and pyrantel pamoate in tablet dosage forms of TOTAL. HPLC separation wascarried out by reversed phase chromatography Betasil C18 (250 mm x 4,6mm i.d.; 5 5m particle size), held at 25°C respective Kromasil 60-5SIL. (250 mm x 4,6 mm i.d.; 5 5m particle size), held at 25°C. The mobile phase consisted of acetonitrile/distilled water (60/40v/v), run at flow rate of 1 mL/min and with UV detection at 210nm, respective acetonitrile/ 0,1% phosphoric acid aq. (60/40 v/v), run at flow rate of 1 mL/ min and with UV detection at 240 nm. Method validation investigated parameters such as linearity (r2=0,9999), range, precision, accuracy and specificity. The described method can be successfully applied for the analysis of TOTAL tablets.http://www.veterinarypharmacon.com/docs/944-2011-ART4.%20eng.pdfpraziquantelpyrantel pamoatereversed phase high performance liquid chromatography |
| spellingShingle | Elena Gabriela Oltean Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate Medicamentul Veterinar praziquantel pyrantel pamoate reversed phase high performance liquid chromatography |
| title | Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate |
| title_full | Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate |
| title_fullStr | Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate |
| title_full_unstemmed | Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate |
| title_short | Development and validation of a RP- HPLC method for the quantitation studies of praziquantel and pyrantel pamoate |
| title_sort | development and validation of a rp hplc method for the quantitation studies of praziquantel and pyrantel pamoate |
| topic | praziquantel pyrantel pamoate reversed phase high performance liquid chromatography |
| url | http://www.veterinarypharmacon.com/docs/944-2011-ART4.%20eng.pdf |
| work_keys_str_mv | AT elenagabrielaoltean developmentandvalidationofarphplcmethodforthequantitationstudiesofpraziquantelandpyrantelpamoate |