A duplex sequencing approach for high-sensitivity detection of genome-edited plantsEuropean Nucleotide Archive (ENA) at EMBL-EBI

A duplex sequencing approach for high-sensitivity detection of genome-edited plantsEuropean Nucleotide Archive (ENA) at EMBL-EBI

In this paper, we have evaluated a targeted high-throughput massive parallel sequencing approach for detecting single nucleotide mutations or small genomic changes generated by new genomic techniques (NGT).We used unique molecular identifiers (UMIs) for the quantification of the mutant alleles and d...

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Bibliographic Details
Main Authors: Laura Bonfini, Moreno Colaiacovo, Cristian Savini, Christoph von Holst, Matteo Maretti, Francesco Gatto, Federica Magni, Paloma Pérez-Bello, Davide Scaglione
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Food Chemistry: Molecular Sciences
Subjects:
Genetically modified organisms (GMOs)
Detection
Duplex sequencing
Solanum lycopersicum
New genomic techniques (NGT)
Next generation sequencing (NGS)
Online Access:http://www.sciencedirect.com/science/article/pii/S2666566225000395
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http://www.sciencedirect.com/science/article/pii/S2666566225000395

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