Steamed root of Rehmannia glutinosa Libosch. relieves methotrexate-induced intestinal mucositis in mice with manninotriose as a core mediator
Chemotherapy-induced intestinal mucositis is a serious complication with few effective treatment options. In Traditional Chinese Medicine (TCM), this toxicity is attributed to Qi and Yin deficiencies, and the steamed root of Rehmannia glutinosa Libosch. (SRR) is commonly prescribed for its therapeut...
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Elsevier
2025-09-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925002609 |
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| author | Ling Wei Yujing Sun Yujiao Li Cory J. Xian Xue-Sen Wen |
| author_facet | Ling Wei Yujing Sun Yujiao Li Cory J. Xian Xue-Sen Wen |
| author_sort | Ling Wei |
| collection | DOAJ |
| description | Chemotherapy-induced intestinal mucositis is a serious complication with few effective treatment options. In Traditional Chinese Medicine (TCM), this toxicity is attributed to Qi and Yin deficiencies, and the steamed root of Rehmannia glutinosa Libosch. (SRR) is commonly prescribed for its therapeutic benefits. This study aimed to evaluate SRR’s effects on methotrexate (MTX)-induced mucositis by modulating the gut microbiota. Mice were administered MTX (300 mg/kg) and SRR (1.56 g/kg), and gut microbiota changes were analysed by 16S rRNA sequencing. Network pharmacology was employed to identify SRR’s active components. SRR alleviated MTX-induced fatigue, diarrhea, weight loss, and intestinal villous damage, and modulated gut microbiota by reducing Clostridium levels, adjusting the Firmicutes/Bacteroidetes ratio, and promoting Gram-positive bacteria. SRR also reduced oxidative damage and inflammation markers (MDA, iNOS and NF-κB) without affecting MTX blood levels. Manninotriose was identified as one of the key active constituents. In conclusion, SRR alleviates MTX-induced intestinal mucositis by exerting antioxidative, anti-inflammatory, and gut microbiota-modulating effects. |
| format | Article |
| id | doaj-art-a8d02770e3394e46ab3bfe3d3114d99d |
| institution | Matheson Library |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-a8d02770e3394e46ab3bfe3d3114d99d2025-07-02T04:50:53ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-09-0111100919Steamed root of Rehmannia glutinosa Libosch. relieves methotrexate-induced intestinal mucositis in mice with manninotriose as a core mediatorLing Wei0Yujing Sun1Yujiao Li2Cory J. Xian3Xue-Sen Wen4State Key Laboratory of Discovery and Utilazation of Functional Components in Traditional Chinese Medicine, Cheeloo College of Medicine, Shandong University, Jinan 250012, PR ChinaDepartment of Endocrinology and Metabolism, Qilu Hospital of Shandong University Jinan, Shandong, 250012, PR ChinaState Key Laboratory of Discovery and Utilazation of Functional Components in Traditional Chinese Medicine, Cheeloo College of Medicine, Shandong University, Jinan 250012, PR ChinaUniSA Clinical and Health Sciences, University of South Australia, Adelaide, SA 5001, Australia; Corresponding authors at: Institute of Pharmacognosy, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, PR China.State Key Laboratory of Discovery and Utilazation of Functional Components in Traditional Chinese Medicine, Cheeloo College of Medicine, Shandong University, Jinan 250012, PR China; Corresponding authors at: Institute of Pharmacognosy, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, PR China.Chemotherapy-induced intestinal mucositis is a serious complication with few effective treatment options. In Traditional Chinese Medicine (TCM), this toxicity is attributed to Qi and Yin deficiencies, and the steamed root of Rehmannia glutinosa Libosch. (SRR) is commonly prescribed for its therapeutic benefits. This study aimed to evaluate SRR’s effects on methotrexate (MTX)-induced mucositis by modulating the gut microbiota. Mice were administered MTX (300 mg/kg) and SRR (1.56 g/kg), and gut microbiota changes were analysed by 16S rRNA sequencing. Network pharmacology was employed to identify SRR’s active components. SRR alleviated MTX-induced fatigue, diarrhea, weight loss, and intestinal villous damage, and modulated gut microbiota by reducing Clostridium levels, adjusting the Firmicutes/Bacteroidetes ratio, and promoting Gram-positive bacteria. SRR also reduced oxidative damage and inflammation markers (MDA, iNOS and NF-κB) without affecting MTX blood levels. Manninotriose was identified as one of the key active constituents. In conclusion, SRR alleviates MTX-induced intestinal mucositis by exerting antioxidative, anti-inflammatory, and gut microbiota-modulating effects.http://www.sciencedirect.com/science/article/pii/S2666893925002609Rehmannia glutinosa Libosch.Methotrexate-induced intestinal mucositisClostridiumFirmicutes/Bacteroidetes ratioManninotrioseProteobacteria and Actinomycetes |
| spellingShingle | Ling Wei Yujing Sun Yujiao Li Cory J. Xian Xue-Sen Wen Steamed root of Rehmannia glutinosa Libosch. relieves methotrexate-induced intestinal mucositis in mice with manninotriose as a core mediator Carbohydrate Polymer Technologies and Applications Rehmannia glutinosa Libosch. Methotrexate-induced intestinal mucositis Clostridium Firmicutes/Bacteroidetes ratio Manninotriose Proteobacteria and Actinomycetes |
| title | Steamed root of Rehmannia glutinosa Libosch. relieves methotrexate-induced intestinal mucositis in mice with manninotriose as a core mediator |
| title_full | Steamed root of Rehmannia glutinosa Libosch. relieves methotrexate-induced intestinal mucositis in mice with manninotriose as a core mediator |
| title_fullStr | Steamed root of Rehmannia glutinosa Libosch. relieves methotrexate-induced intestinal mucositis in mice with manninotriose as a core mediator |
| title_full_unstemmed | Steamed root of Rehmannia glutinosa Libosch. relieves methotrexate-induced intestinal mucositis in mice with manninotriose as a core mediator |
| title_short | Steamed root of Rehmannia glutinosa Libosch. relieves methotrexate-induced intestinal mucositis in mice with manninotriose as a core mediator |
| title_sort | steamed root of rehmannia glutinosa libosch relieves methotrexate induced intestinal mucositis in mice with manninotriose as a core mediator |
| topic | Rehmannia glutinosa Libosch. Methotrexate-induced intestinal mucositis Clostridium Firmicutes/Bacteroidetes ratio Manninotriose Proteobacteria and Actinomycetes |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925002609 |
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