Biotechnological techniques for intensification of protein extraction from the porcine pancreas
Processing of secondary products after slaughter of farm animals is in demand. The pancreas is a rich source of bioactive protein substances, effective extraction of which is a serious problem today due to their aggregation. The aim of the work was to assess the extractivity of protein substances of...
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| Format: | Article |
| Language: | English |
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The V.M. Gorbatov All-Russian Meat Research Institute
2022-12-01
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| Series: | Теория и практика переработки мяса |
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| Online Access: | https://www.meatjournal.ru/jour/article/view/236 |
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| author | E. A. Kotenkova A. G. Akhremko E. K. Polishchuk M. A. Aryuzina M. E. Spirina |
| author_facet | E. A. Kotenkova A. G. Akhremko E. K. Polishchuk M. A. Aryuzina M. E. Spirina |
| author_sort | E. A. Kotenkova |
| collection | DOAJ |
| description | Processing of secondary products after slaughter of farm animals is in demand. The pancreas is a rich source of bioactive protein substances, effective extraction of which is a serious problem today due to their aggregation. The aim of the work was to assess the extractivity of protein substances of the porcine pancreas using sodium chloride, trehalose, arginine, and combination of glycine and proline. The protein concentration was determined in the obtained extracts by the biuret reaction and their protein composition was assessed by densitometry of two-dimensional electropherograms using software ImageMaster™ 2D Platinum powered by Melanie 8.0. The results showed a positive effect of anti-aggregation agents on the release of protein substances into a solution. The highest protein concentration (33.36±0.64 g/l) was observed when adding 1М L-arginine; however, it was conditioned mainly by an increase in the content of three major protein fractions rather than by diversity of the protein composition. In general, the use of 0.9% NaCl as an extractive agent was quite effective, but selectivity to certain protein groups was observed for anti-aggregation agents such as sodium chloride, trehalose, arginine, glycine and proline, as well as their combination. The obtained results are important for intensifying extraction of protein substances including target ones with the subsequent application in different fields. |
| format | Article |
| id | doaj-art-a8bdd4c0f2674ff4bf7cb0cfbcb45bc7 |
| institution | Matheson Library |
| issn | 2414-438X 2414-441X |
| language | English |
| publishDate | 2022-12-01 |
| publisher | The V.M. Gorbatov All-Russian Meat Research Institute |
| record_format | Article |
| series | Теория и практика переработки мяса |
| spelling | doaj-art-a8bdd4c0f2674ff4bf7cb0cfbcb45bc72025-08-04T13:11:45ZengThe V.M. Gorbatov All-Russian Meat Research InstituteТеория и практика переработки мяса2414-438X2414-441X2022-12-017425826410.21323/2414-438X-2022-7-4-258-264202Biotechnological techniques for intensification of protein extraction from the porcine pancreasE. A. Kotenkova0A. G. Akhremko1E. K. Polishchuk2M. A. Aryuzina3M. E. Spirina4V. M. Gorbatov Federal Research Center for Food SystemsV. M. Gorbatov Federal Research Center for Food SystemsV. M. Gorbatov Federal Research Center for Food SystemsV. M. Gorbatov Federal Research Center for Food SystemsV. M. Gorbatov Federal Research Center for Food SystemsProcessing of secondary products after slaughter of farm animals is in demand. The pancreas is a rich source of bioactive protein substances, effective extraction of which is a serious problem today due to their aggregation. The aim of the work was to assess the extractivity of protein substances of the porcine pancreas using sodium chloride, trehalose, arginine, and combination of glycine and proline. The protein concentration was determined in the obtained extracts by the biuret reaction and their protein composition was assessed by densitometry of two-dimensional electropherograms using software ImageMaster™ 2D Platinum powered by Melanie 8.0. The results showed a positive effect of anti-aggregation agents on the release of protein substances into a solution. The highest protein concentration (33.36±0.64 g/l) was observed when adding 1М L-arginine; however, it was conditioned mainly by an increase in the content of three major protein fractions rather than by diversity of the protein composition. In general, the use of 0.9% NaCl as an extractive agent was quite effective, but selectivity to certain protein groups was observed for anti-aggregation agents such as sodium chloride, trehalose, arginine, glycine and proline, as well as their combination. The obtained results are important for intensifying extraction of protein substances including target ones with the subsequent application in different fields.https://www.meatjournal.ru/jour/article/view/236aggregationanti-aggregation agentextractionprotein2-detrehalosearginineglycineproline |
| spellingShingle | E. A. Kotenkova A. G. Akhremko E. K. Polishchuk M. A. Aryuzina M. E. Spirina Biotechnological techniques for intensification of protein extraction from the porcine pancreas Теория и практика переработки мяса aggregation anti-aggregation agent extraction protein 2-de trehalose arginine glycine proline |
| title | Biotechnological techniques for intensification of protein extraction from the porcine pancreas |
| title_full | Biotechnological techniques for intensification of protein extraction from the porcine pancreas |
| title_fullStr | Biotechnological techniques for intensification of protein extraction from the porcine pancreas |
| title_full_unstemmed | Biotechnological techniques for intensification of protein extraction from the porcine pancreas |
| title_short | Biotechnological techniques for intensification of protein extraction from the porcine pancreas |
| title_sort | biotechnological techniques for intensification of protein extraction from the porcine pancreas |
| topic | aggregation anti-aggregation agent extraction protein 2-de trehalose arginine glycine proline |
| url | https://www.meatjournal.ru/jour/article/view/236 |
| work_keys_str_mv | AT eakotenkova biotechnologicaltechniquesforintensificationofproteinextractionfromtheporcinepancreas AT agakhremko biotechnologicaltechniquesforintensificationofproteinextractionfromtheporcinepancreas AT ekpolishchuk biotechnologicaltechniquesforintensificationofproteinextractionfromtheporcinepancreas AT maaryuzina biotechnologicaltechniquesforintensificationofproteinextractionfromtheporcinepancreas AT mespirina biotechnologicaltechniquesforintensificationofproteinextractionfromtheporcinepancreas |