A Panel of Cultures of Cancer and Normal Lung Cells with a Characterized Expression of PD-L1 to Search and Development of the Target Modifiers of Immunotherapy
Background. The search for effective combinations of immune checkpoint inhibitors with common cytostatics, targeted cancer drugs and other treatments is a modern trend to improve the effectiveness of immunotherapy.Purpose. Development of a panel of lung cancer cell cultures and cells of normal lung ti...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
LLC "Publishing House OKI"
2024-11-01
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| Series: | Антибиотики и Химиотерапия |
| Subjects: | |
| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/1158 |
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| Summary: | Background. The search for effective combinations of immune checkpoint inhibitors with common cytostatics, targeted cancer drugs and other treatments is a modern trend to improve the effectiveness of immunotherapy.Purpose. Development of a panel of lung cancer cell cultures and cells of normal lung tissue with a characterized molecular phenotype by expression of one of the targets of immunotherapy — programmed cell death ligand 1 (PD-L1).Methods. PD-L1 expression was quantitatively analyzed by immunofluorescence method associated with flow cytometry.Results. A panel of lung cancer cell cultures of different histotypes and cells of normal lung tissue with characterized molecular phenotype was formed according to the expression of the immunotherapy target PD-L1. In terms of PD-L1 expression intensity, cell cultures can be arranged in a series: Calu-1 HFL-1 Calu-6 Wi-26 A-549 H-596 H-211, with a 7-fold difference in the index between Calu-1 and H-211.Conclusion. The panel of cultures of cancer and normal lung cells is recommended for the search and development of effective modifiers of the immunotherapy target PD-L1. |
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| ISSN: | 0235-2990 |