RT-QuIC Reactivity in the Brain and Other Organs and Tissues in Early, Middle-early, Middle-late and Terminal Stages of Scrapie Agent 263K Intracerebral Infection in Hamsters
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Compuscript Ltd
2025-02-01
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| Series: | Zoonoses |
| Online Access: | https://www.scienceopen.com/hosted-document?doi=10.15212/ZOONOSES-2024-0033 |
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| Summary: | <div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section">
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<h5 class="section-title" id="d1447294e220">Objective:</h5>
<p dir="auto" id="d1447294e222">This study was aimed at analyzing the distribution and progression of prion seeds
across the central nervous system (CNS) and peripheral tissues, by evaluating the
real time quaking-induced conversion (RT-QuIC) reactivity of the brain, and other
organs and tissues, from hamsters in early, middle-early, middle-late and terminal
stages of intracerebral infection with scrapie agent 263K.
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<h5 class="section-title" id="d1447294e225">Methods:</h5>
<p dir="auto" id="d1447294e227">Brain, skin and other organ specimens were collected at approximately 20, 40, 60 and
80 (terminal stage) days post-inoculation, and homogenates were prepared. Protein
misfolding cyclic amplification (PMCA) and RT-QuIC were used for sample detection.
The 50% seeding dose (SD
<sub>50</sub>) values for prion seeding were calculated with the Spearman-Karber method, and RT-QuIC
reactivity lag times were compared among tissues and time points.
</p>
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<h5 class="section-title" id="d1447294e233">Results:</h5>
<p dir="auto" id="d1447294e235">RT-QuIC indicated positive reactions in brain tissue samples across all time points
(20–80 dpi), and increasing seeding capacity and decreasing lag times were observed
during the incubation period. In peripheral tissues, including the heart, liver, spleen,
lung, colon and skin, RT-QuIC positivity showed delayed onset, appearing at later
stages (≥40 dpi) than observed in the brain. The brain exhibited significantly higher
SD
<sub>50</sub> values than peripheral tissues, thus reflecting greater seeding capacity. Prion seeds
were widely distributed in the CNS and peripheral tissues at the terminal stage, and
the highest RT-QuIC positivity and SD
<sub>50</sub> values were observed in the brain.
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<h5 class="section-title" id="d1447294e244">Conclusions:</h5>
<p dir="auto" id="d1447294e246">Prions were widely distributed in the CNS and peripheral tissues of scrapie-infected
hamsters, and CNS tissues exhibited a particularly high level of reactivity and seeding
capacity in the RT-QuIC assay.
</p>
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| ISSN: | 2737-7466 2737-7474 |