Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria
Background: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a significant zoonotic pathogen implicated in severe human illnesses such as hemorrhagic colitis and hemolytic uremic syndrome (HUS). Cattle are known asymptomatic reservoirs, and their fecal shedding of the pathogen poses a public...
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Zagazig University, Faculty of Medicine
2025-08-01
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| Series: | Microbes and Infectious Diseases |
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| author | ABDULRAZAK LAWAL Lawan Kabir Fatima Balarabe Yusuf Abubakar Mannir Usman Fatima Lawal Yuguda Umar |
| author_facet | ABDULRAZAK LAWAL Lawan Kabir Fatima Balarabe Yusuf Abubakar Mannir Usman Fatima Lawal Yuguda Umar |
| author_sort | ABDULRAZAK LAWAL |
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| description | Background: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a significant zoonotic pathogen implicated in severe human illnesses such as hemorrhagic colitis and hemolytic uremic syndrome (HUS). Cattle are known asymptomatic reservoirs, and their fecal shedding of the pathogen poses a public health risk through contamination of food and water sources. This study aimed to isolate and molecularly characterize STEC O157:H7 from cattle feces within Zaria Metropolis, Nigeria. Methods: A cross sectional study was conducted. A total of 328 cattle faecal samples were collected from Eight (8) different Fulani cattle herds. Faecal samples where enriched, selectively cultured, and screened for E. coli O157:H7 using Wellcolex rapid latex agglutination kit and multiplex polymerase chain reaction (PCR) to detect stx1, stx2, eaeA and hlyA genes. Results: A total of 26 (7.93%) isolates were positive for E. coli O157:H7 using Wellcolex rapid latex agglutination kit. Out of which, 1(0.31%) isolate was found in bull while 25(7.62%) were in cows, 3(0.91%) isolates were in calves while 23(7.02%) in adult cattle and 4(1.22%) were in Bokoloji while 22(6.71%) were in Bunaji, a total 26 isolates were subjected to multiplex PCR, only 2(7.69%) expressed the shiga toxin genes (stx1). Conclusion: This study highlights the presence of shiga toxin producing E. coli O157:H7 in the cattle population of Zaria and the need for stringent control measures to prevent transmission to humans. |
| format | Article |
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| language | English |
| publishDate | 2025-08-01 |
| publisher | Zagazig University, Faculty of Medicine |
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| series | Microbes and Infectious Diseases |
| spelling | doaj-art-a72454d7cfce4ed3947d47a01794ece12025-08-03T15:31:45ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402025-08-01635020502810.21608/mid.2024.323848.2248393927Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, NigeriaABDULRAZAK LAWAL0Lawan Kabir1Fatima Balarabe2Yusuf Abubakar3Mannir Usman4Fatima Lawal5Yuguda Umar6Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Bayero University Kano, Nigeria.Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University Zaria, Nigeria.Department of scientific and industrial research, National Research Institute For Chemical Technology Zaria, Nigeria.Federal University of Agriculture Zuru, Zuru, Kebbi State, NigeriaFaculty of Veterinary Medicine, Bayero University, Kano NigeriaDepartment of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University Zaria, Nigeria.Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Bayero University Kano, Nigeria.Background: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a significant zoonotic pathogen implicated in severe human illnesses such as hemorrhagic colitis and hemolytic uremic syndrome (HUS). Cattle are known asymptomatic reservoirs, and their fecal shedding of the pathogen poses a public health risk through contamination of food and water sources. This study aimed to isolate and molecularly characterize STEC O157:H7 from cattle feces within Zaria Metropolis, Nigeria. Methods: A cross sectional study was conducted. A total of 328 cattle faecal samples were collected from Eight (8) different Fulani cattle herds. Faecal samples where enriched, selectively cultured, and screened for E. coli O157:H7 using Wellcolex rapid latex agglutination kit and multiplex polymerase chain reaction (PCR) to detect stx1, stx2, eaeA and hlyA genes. Results: A total of 26 (7.93%) isolates were positive for E. coli O157:H7 using Wellcolex rapid latex agglutination kit. Out of which, 1(0.31%) isolate was found in bull while 25(7.62%) were in cows, 3(0.91%) isolates were in calves while 23(7.02%) in adult cattle and 4(1.22%) were in Bokoloji while 22(6.71%) were in Bunaji, a total 26 isolates were subjected to multiplex PCR, only 2(7.69%) expressed the shiga toxin genes (stx1). Conclusion: This study highlights the presence of shiga toxin producing E. coli O157:H7 in the cattle population of Zaria and the need for stringent control measures to prevent transmission to humans.https://mid.journals.ekb.eg/article_393927_de5b77f2bdc9a5df97dbe373790bd4dd.pdfescherichia coli o157:h7shiga toxinzoonosislatex agglutinationmultiplex pcr |
| spellingShingle | ABDULRAZAK LAWAL Lawan Kabir Fatima Balarabe Yusuf Abubakar Mannir Usman Fatima Lawal Yuguda Umar Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria Microbes and Infectious Diseases escherichia coli o157:h7 shiga toxin zoonosis latex agglutination multiplex pcr |
| title | Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria |
| title_full | Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria |
| title_fullStr | Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria |
| title_full_unstemmed | Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria |
| title_short | Isolation and characterization of shiga toxin-producing Escherichia coli O157:H7 from cattle feces within Zaria Metropolis, Nigeria |
| title_sort | isolation and characterization of shiga toxin producing escherichia coli o157 h7 from cattle feces within zaria metropolis nigeria |
| topic | escherichia coli o157:h7 shiga toxin zoonosis latex agglutination multiplex pcr |
| url | https://mid.journals.ekb.eg/article_393927_de5b77f2bdc9a5df97dbe373790bd4dd.pdf |
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