Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens
Effects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium...
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Format: | Article |
Language: | English |
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Zhejiang University Press
2007-11-01
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Series: | 浙江大学学报. 农业与生命科学版 |
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Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0591 |
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author | LIU Yong-li LONG Zi-li GAO Yue Takashi Harada |
author_facet | LIU Yong-li LONG Zi-li GAO Yue Takashi Harada |
author_sort | LIU Yong-li |
collection | DOAJ |
description | Effects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium (MS) supplemented with 0.1-10 μmol·L<sup>-1</sup> α-naphthaleneacetic acid (NAA) and 10 μmol· L<sup>-1</sup> N-6-benzyladenine (BA). Rooted shoots grew into vigorous plantlets after transferred to 1/2 MS medium containing 1 μmol·L<sup>-1</sup> NAA or 1 μmol·L<sup>-1</sup> indolebutyric acid (IBA). The rooted plantlets were transplanted to the field, grew very vigorous and reached 27 cm height after 8 months. |
format | Article |
id | doaj-art-a7095f79d51a4c71b561c9c32e6a56fe |
institution | Matheson Library |
issn | 1008-9209 2097-5155 |
language | English |
publishDate | 2007-11-01 |
publisher | Zhejiang University Press |
record_format | Article |
series | 浙江大学学报. 农业与生命科学版 |
spelling | doaj-art-a7095f79d51a4c71b561c9c32e6a56fe2025-08-01T05:36:19ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552007-11-013359159610.3785/1008-9209.2007.06.059110089209Organ formation and plant regeneration in vitro tissue culture of Crassula arborescensLIU Yong-liLONG Zi-liGAO YueTakashi HaradaEffects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium (MS) supplemented with 0.1-10 μmol·L<sup>-1</sup> α-naphthaleneacetic acid (NAA) and 10 μmol· L<sup>-1</sup> N-6-benzyladenine (BA). Rooted shoots grew into vigorous plantlets after transferred to 1/2 MS medium containing 1 μmol·L<sup>-1</sup> NAA or 1 μmol·L<sup>-1</sup> indolebutyric acid (IBA). The rooted plantlets were transplanted to the field, grew very vigorous and reached 27 cm height after 8 months.https://www.academax.com/doi/10.3785/1008-9209.2007.06.0591<italic>Crassula arborescens</italic>plant growth regulatorsshoot |
spellingShingle | LIU Yong-li LONG Zi-li GAO Yue Takashi Harada Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens 浙江大学学报. 农业与生命科学版 <italic>Crassula arborescens</italic> plant growth regulators shoot |
title | Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens |
title_full | Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens |
title_fullStr | Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens |
title_full_unstemmed | Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens |
title_short | Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens |
title_sort | organ formation and plant regeneration in vitro tissue culture of crassula arborescens |
topic | <italic>Crassula arborescens</italic> plant growth regulators shoot |
url | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0591 |
work_keys_str_mv | AT liuyongli organformationandplantregenerationinvitrotissuecultureofcrassulaarborescens AT longzili organformationandplantregenerationinvitrotissuecultureofcrassulaarborescens AT gaoyue organformationandplantregenerationinvitrotissuecultureofcrassulaarborescens AT takashiharada organformationandplantregenerationinvitrotissuecultureofcrassulaarborescens |