Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens

Effects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium...

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Main Authors: LIU Yong-li, LONG Zi-li, GAO Yue, Takashi Harada
Format: Article
Language:English
Published: Zhejiang University Press 2007-11-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
Online Access:https://www.academax.com/doi/10.3785/1008-9209.2007.06.0591
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author LIU Yong-li
LONG Zi-li
GAO Yue
Takashi Harada
author_facet LIU Yong-li
LONG Zi-li
GAO Yue
Takashi Harada
author_sort LIU Yong-li
collection DOAJ
description Effects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium (MS) supplemented with 0.1-10 μmol·L<sup>-1</sup> α-naphthaleneacetic acid (NAA) and 10 μmol· L<sup>-1</sup> N-6-benzyladenine (BA). Rooted shoots grew into vigorous plantlets after transferred to 1/2 MS medium containing 1 μmol·L<sup>-1</sup> NAA or 1 μmol·L<sup>-1</sup> indolebutyric acid (IBA). The rooted plantlets were transplanted to the field, grew very vigorous and reached 27 cm height after 8 months.
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series 浙江大学学报. 农业与生命科学版
spelling doaj-art-a7095f79d51a4c71b561c9c32e6a56fe2025-08-01T05:36:19ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552007-11-013359159610.3785/1008-9209.2007.06.059110089209Organ formation and plant regeneration in vitro tissue culture of Crassula arborescensLIU Yong-liLONG Zi-liGAO YueTakashi HaradaEffects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium (MS) supplemented with 0.1-10 μmol·L<sup>-1</sup> α-naphthaleneacetic acid (NAA) and 10 μmol· L<sup>-1</sup> N-6-benzyladenine (BA). Rooted shoots grew into vigorous plantlets after transferred to 1/2 MS medium containing 1 μmol·L<sup>-1</sup> NAA or 1 μmol·L<sup>-1</sup> indolebutyric acid (IBA). The rooted plantlets were transplanted to the field, grew very vigorous and reached 27 cm height after 8 months.https://www.academax.com/doi/10.3785/1008-9209.2007.06.0591<italic>Crassula arborescens</italic>plant growth regulatorsshoot
spellingShingle LIU Yong-li
LONG Zi-li
GAO Yue
Takashi Harada
Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens
浙江大学学报. 农业与生命科学版
<italic>Crassula arborescens</italic>
plant growth regulators
shoot
title Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens
title_full Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens
title_fullStr Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens
title_full_unstemmed Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens
title_short Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens
title_sort organ formation and plant regeneration in vitro tissue culture of crassula arborescens
topic <italic>Crassula arborescens</italic>
plant growth regulators
shoot
url https://www.academax.com/doi/10.3785/1008-9209.2007.06.0591
work_keys_str_mv AT liuyongli organformationandplantregenerationinvitrotissuecultureofcrassulaarborescens
AT longzili organformationandplantregenerationinvitrotissuecultureofcrassulaarborescens
AT gaoyue organformationandplantregenerationinvitrotissuecultureofcrassulaarborescens
AT takashiharada organformationandplantregenerationinvitrotissuecultureofcrassulaarborescens