The potential of wastewater monitoring as a novel surveillance tool for early warning of Bordetella pertussis outbreaks
The prolonged latency period and delayed reporting of clinical surveillance data have led to a sluggish response to Bordetella pertussis outbreaks in China. Wastewater-based surveillance (WBS) provides real-time, cost-effective monitoring of various viruses. To confirm whether this approach can be a...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2025.2528537 |
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| author | Songzhe Fu Xin Du Zheng Xu Haifeng Li Hui Yao Shuyue Tang Wenjuan Zhao Min Hao Zhiguang Qiu Zengguo Wang |
| author_facet | Songzhe Fu Xin Du Zheng Xu Haifeng Li Hui Yao Shuyue Tang Wenjuan Zhao Min Hao Zhiguang Qiu Zengguo Wang |
| author_sort | Songzhe Fu |
| collection | DOAJ |
| description | The prolonged latency period and delayed reporting of clinical surveillance data have led to a sluggish response to Bordetella pertussis outbreaks in China. Wastewater-based surveillance (WBS) provides real-time, cost-effective monitoring of various viruses. To confirm whether this approach can be applied to B. pertussis, 271 patients infected with B. pertussis were enrolled to evaluate the fecal shedding patterns of the bacterium. Concurrently, we collected wastewater samples in a typical northern (Xi’an) and southern city (Nanchang) in China, from September 2023 to July 2024. The concentrations of B. pertussis in the sewage were detected using quantitative polymerase chain reaction (qPCR). Additionally, metagenomic sequencing with pre-enrichment was employed to trace genotypes of B. pertussis. We report that 40.2% of cases tested positive for B. pertussis in fecal samples. Sewage surveillance identified two waves of B. pertussis infection in Xi’an and one wave of B. pertussis outbreak in Nanchang. The concentration of B. pertussis in sewage showed a strong correlation with the dynamics of diagnosed cases. Notably, each peak of sewage B. pertussis concentration occurred 10 days prior to clinical surveillance, underscoring the value of WBS in early warning. Additionally, both clinical and wastewater surveillance demonstrated consistency in the prevalence estimation of B. pertussis infection cases. Finally, relative to clinical surveillance, wastewater sequencing also revealed the same genotypes of B. pertussis. This study presents the first comprehensive analysis of the fecal shedding of B. pertussis, thereby establishing the potential for early detection of B. pertussis outbreaks through WBS. |
| format | Article |
| id | doaj-art-a6b13bf1bd9a40e18263821e7a3fcaf4 |
| institution | Matheson Library |
| issn | 2222-1751 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-a6b13bf1bd9a40e18263821e7a3fcaf42025-07-10T14:58:12ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512025-12-0114110.1080/22221751.2025.2528537The potential of wastewater monitoring as a novel surveillance tool for early warning of Bordetella pertussis outbreaksSongzhe Fu0Xin Du1Zheng Xu2Haifeng Li3Hui Yao4Shuyue Tang5Wenjuan Zhao6Min Hao7Zhiguang Qiu8Zengguo Wang9Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an, People’s Republic of ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an, People’s Republic of ChinaInstitute of Infectious Diseases, Shenzhen Bay Laboratory, Shenzhen, People’s Republic of ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi'an, People’s Republic of ChinaDepartment of Clinical Laboratory, Xi'an Children's Hospital, Affiliated Children's Hospital of Xi'an Jiaotong University, Xi'an, People’s Republic of ChinaSchool of Health Public, Shaanxi University of Chinese Medicine, Xianyang, People’s Republic of ChinaSchool of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang, People’s Republic of ChinaXi’an Medical University, Xi’an, People’s Republic of ChinaSchool of Environment and Energy, Shenzhen Graduate School, Peking University, Shenzhen, People’s Republic of ChinaDepartment of Clinical Laboratory, Xi'an Children's Hospital, Affiliated Children's Hospital of Xi'an Jiaotong University, Xi'an, People’s Republic of ChinaThe prolonged latency period and delayed reporting of clinical surveillance data have led to a sluggish response to Bordetella pertussis outbreaks in China. Wastewater-based surveillance (WBS) provides real-time, cost-effective monitoring of various viruses. To confirm whether this approach can be applied to B. pertussis, 271 patients infected with B. pertussis were enrolled to evaluate the fecal shedding patterns of the bacterium. Concurrently, we collected wastewater samples in a typical northern (Xi’an) and southern city (Nanchang) in China, from September 2023 to July 2024. The concentrations of B. pertussis in the sewage were detected using quantitative polymerase chain reaction (qPCR). Additionally, metagenomic sequencing with pre-enrichment was employed to trace genotypes of B. pertussis. We report that 40.2% of cases tested positive for B. pertussis in fecal samples. Sewage surveillance identified two waves of B. pertussis infection in Xi’an and one wave of B. pertussis outbreak in Nanchang. The concentration of B. pertussis in sewage showed a strong correlation with the dynamics of diagnosed cases. Notably, each peak of sewage B. pertussis concentration occurred 10 days prior to clinical surveillance, underscoring the value of WBS in early warning. Additionally, both clinical and wastewater surveillance demonstrated consistency in the prevalence estimation of B. pertussis infection cases. Finally, relative to clinical surveillance, wastewater sequencing also revealed the same genotypes of B. pertussis. This study presents the first comprehensive analysis of the fecal shedding of B. pertussis, thereby establishing the potential for early detection of B. pertussis outbreaks through WBS.https://www.tandfonline.com/doi/10.1080/22221751.2025.2528537Bordetella pertussisWastewater based surveillancefaecal sheddingearly warningprevalence estimation |
| spellingShingle | Songzhe Fu Xin Du Zheng Xu Haifeng Li Hui Yao Shuyue Tang Wenjuan Zhao Min Hao Zhiguang Qiu Zengguo Wang The potential of wastewater monitoring as a novel surveillance tool for early warning of Bordetella pertussis outbreaks Emerging Microbes and Infections Bordetella pertussis Wastewater based surveillance faecal shedding early warning prevalence estimation |
| title | The potential of wastewater monitoring as a novel surveillance tool for early warning of Bordetella pertussis outbreaks |
| title_full | The potential of wastewater monitoring as a novel surveillance tool for early warning of Bordetella pertussis outbreaks |
| title_fullStr | The potential of wastewater monitoring as a novel surveillance tool for early warning of Bordetella pertussis outbreaks |
| title_full_unstemmed | The potential of wastewater monitoring as a novel surveillance tool for early warning of Bordetella pertussis outbreaks |
| title_short | The potential of wastewater monitoring as a novel surveillance tool for early warning of Bordetella pertussis outbreaks |
| title_sort | potential of wastewater monitoring as a novel surveillance tool for early warning of bordetella pertussis outbreaks |
| topic | Bordetella pertussis Wastewater based surveillance faecal shedding early warning prevalence estimation |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2025.2528537 |
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