Gene mapping and candidate gene analysis of multi-floret spikelet 3 (mfs3) in rice (Oryza sativa L.)
Rice (Oryza sativa L.) is one of the most important food crops worldwide and a model monocot plant for gene function analysis, so it is an ideal system for studying flower development. This study reports a mutant, named multi-floret spikelet 3 (mfs3), which is related to the spikelet development in...
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KeAi Communications Co., Ltd.
2019-12-01
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| Rangatū: | Journal of Integrative Agriculture |
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| Urunga tuihono: | http://www.sciencedirect.com/science/article/pii/S2095311919626523 |
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| author | Hao ZHENG Jun ZHANG Hui ZHUANG Xiao-qin ZENG Jun TANG Hong-lei WANG Huan CHEN Yan LI Ying-hua LING Guang-hua HE Yun-feng LI |
| author_facet | Hao ZHENG Jun ZHANG Hui ZHUANG Xiao-qin ZENG Jun TANG Hong-lei WANG Huan CHEN Yan LI Ying-hua LING Guang-hua HE Yun-feng LI |
| author_sort | Hao ZHENG |
| collection | DOAJ |
| description | Rice (Oryza sativa L.) is one of the most important food crops worldwide and a model monocot plant for gene function analysis, so it is an ideal system for studying flower development. This study reports a mutant, named multi-floret spikelet 3 (mfs3), which is related to the spikelet development in rice and derived from the ethylmethane sulfonate (EMS)-treated rice cultivar XIDA 1B. In mfs3, the main body of palea (bop) was degenerated severely and only glume-like marginal regions of palea (mrp) remained, while other floral organs developed normally, indicating that the palea identity was seriously influenced by the mutation. It was also observed that the number of floral organs was increased in some spikelets, including 2 lemmas, 4 mrp, 4 lodicules, 8–10 stamens, and 2 pistils, which meant that the spikelet determinacy was lost to some degree in mfs3. Furthermore, genetic analysis demonstrated that the mfs3 trait was controlled by a single recessive gene. Using 426 F2 mutants derived from the cross between sterile line 56S and mfs3, the MULTI-FLORET SPIKELET 3 (MFS3) gene was mapped between the molecular markers RM19347 and RM19352 on Chr.6, with a physical distance of 106.3 kb. Sequencing of candidate genes revealed that an 83-bp fragment loss and a base substitution occurred in the LOC_Os06g04540 gene in the mutant, confirming preliminarily that the LOC_Os06g04540 gene was the MFS3 candidate gene. Subsequent qPCR analysis showed that the mutation caused the down-regulation of OsMADS1 and FON1 genes, and the up-regulation of OsIDS1 and SNB genes, which are all involved in the regulation of spikelet development. The MFS3 mutation also significantly reduced the transcription of the REP gene, which is involved in palea development. These results indicated that the MFS3 gene might be involved in the spikelet meristem determinacy and palea identity by regulating the expression of these related genes. |
| format | Article |
| id | doaj-art-a5ec561ff9cf4e2f8f67fee476ff4ebf |
| institution | Matheson Library |
| issn | 2095-3119 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-a5ec561ff9cf4e2f8f67fee476ff4ebf2025-08-02T15:54:03ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192019-12-01181226732681Gene mapping and candidate gene analysis of multi-floret spikelet 3 (mfs3) in rice (Oryza sativa L.)Hao ZHENG0Jun ZHANG1Hui ZHUANG2Xiao-qin ZENG3Jun TANG4Hong-lei WANG5Huan CHEN6Yan LI7Ying-hua LING8Guang-hua HE9Yun-feng LI10Correspondence ZHENG Hao, +86-23-68250486; Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaChongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaCorrespondence LI Yun-feng, Tel: +86-23-68250486; Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops/Rice Research Institute, Southwest University, Chongqing 400715, P.R.ChinaRice (Oryza sativa L.) is one of the most important food crops worldwide and a model monocot plant for gene function analysis, so it is an ideal system for studying flower development. This study reports a mutant, named multi-floret spikelet 3 (mfs3), which is related to the spikelet development in rice and derived from the ethylmethane sulfonate (EMS)-treated rice cultivar XIDA 1B. In mfs3, the main body of palea (bop) was degenerated severely and only glume-like marginal regions of palea (mrp) remained, while other floral organs developed normally, indicating that the palea identity was seriously influenced by the mutation. It was also observed that the number of floral organs was increased in some spikelets, including 2 lemmas, 4 mrp, 4 lodicules, 8–10 stamens, and 2 pistils, which meant that the spikelet determinacy was lost to some degree in mfs3. Furthermore, genetic analysis demonstrated that the mfs3 trait was controlled by a single recessive gene. Using 426 F2 mutants derived from the cross between sterile line 56S and mfs3, the MULTI-FLORET SPIKELET 3 (MFS3) gene was mapped between the molecular markers RM19347 and RM19352 on Chr.6, with a physical distance of 106.3 kb. Sequencing of candidate genes revealed that an 83-bp fragment loss and a base substitution occurred in the LOC_Os06g04540 gene in the mutant, confirming preliminarily that the LOC_Os06g04540 gene was the MFS3 candidate gene. Subsequent qPCR analysis showed that the mutation caused the down-regulation of OsMADS1 and FON1 genes, and the up-regulation of OsIDS1 and SNB genes, which are all involved in the regulation of spikelet development. The MFS3 mutation also significantly reduced the transcription of the REP gene, which is involved in palea development. These results indicated that the MFS3 gene might be involved in the spikelet meristem determinacy and palea identity by regulating the expression of these related genes.http://www.sciencedirect.com/science/article/pii/S2095311919626523rice (Oryza sativa)multi-floret spikeletpalea degenerationgene mappingcandidate gene |
| spellingShingle | Hao ZHENG Jun ZHANG Hui ZHUANG Xiao-qin ZENG Jun TANG Hong-lei WANG Huan CHEN Yan LI Ying-hua LING Guang-hua HE Yun-feng LI Gene mapping and candidate gene analysis of multi-floret spikelet 3 (mfs3) in rice (Oryza sativa L.) Journal of Integrative Agriculture rice (Oryza sativa) multi-floret spikelet palea degeneration gene mapping candidate gene |
| title | Gene mapping and candidate gene analysis of multi-floret spikelet 3 (mfs3) in rice (Oryza sativa L.) |
| title_full | Gene mapping and candidate gene analysis of multi-floret spikelet 3 (mfs3) in rice (Oryza sativa L.) |
| title_fullStr | Gene mapping and candidate gene analysis of multi-floret spikelet 3 (mfs3) in rice (Oryza sativa L.) |
| title_full_unstemmed | Gene mapping and candidate gene analysis of multi-floret spikelet 3 (mfs3) in rice (Oryza sativa L.) |
| title_short | Gene mapping and candidate gene analysis of multi-floret spikelet 3 (mfs3) in rice (Oryza sativa L.) |
| title_sort | gene mapping and candidate gene analysis of multi floret spikelet 3 mfs3 in rice oryza sativa l |
| topic | rice (Oryza sativa) multi-floret spikelet palea degeneration gene mapping candidate gene |
| url | http://www.sciencedirect.com/science/article/pii/S2095311919626523 |
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