Local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detector
Optical-resolution optoacoustic (photoacoustic) microscopy is a hybrid imaging modality combining focused optical excitation with ultrasound detection, thus achieving micrometer-scale spatial resolution and high-contrast angiographic imaging. Despite these important advantages, maintaining safe lase...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Photoacoustics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597925000576 |
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| author | Daria Voitovich Alexey Kurnikov Anna Orlova Aleksej Petushkov Liubov Shimolina Anastasia Komarova Marina Shirmanova Yu-Hang Liu Daniel Razansky Pavel Subochev |
| author_facet | Daria Voitovich Alexey Kurnikov Anna Orlova Aleksej Petushkov Liubov Shimolina Anastasia Komarova Marina Shirmanova Yu-Hang Liu Daniel Razansky Pavel Subochev |
| author_sort | Daria Voitovich |
| collection | DOAJ |
| description | Optical-resolution optoacoustic (photoacoustic) microscopy is a hybrid imaging modality combining focused optical excitation with ultrasound detection, thus achieving micrometer-scale spatial resolution and high-contrast angiographic imaging. Despite these important advantages, maintaining safe laser fluence levels is essential to prevent tissue damage while ensuring sufficient detection sensitivity. Here, we introduce a model that directly relates the detector’s noise-equivalent pressure (NEP) to the local laser fluence at the imaged blood vessel. The model incorporates acoustic propagation effects from an optoacoustic source to a spherically focused detector with limited aperture and bandwidth, offering a more comprehensive understanding of how fluence and ultrasonic sensitivity are interconnected. The effects of ultrasound generation propagation and detection were accounted for using analytical estimations and numerical simulations, while detector's NEP was experimentally measured with a calibrated hydrophone. The proposed model for evaluating of local laser fluence with a calibrated ultrasound detector was validated through in vitro experiments with superficially located blood layer and numerical Monte Carlo/k-Wave simulations featuring deeper vessels. In vivo experiments employing 532 nm laser excitation and wideband 1–30 MHz ultrasonic detection further demonstrated the model’s capacity for real-time adjustments of laser parameters to ensure tissue safety. |
| format | Article |
| id | doaj-art-a58b4c40b32741c2ac7d6eefc0c1b621 |
| institution | Matheson Library |
| issn | 2213-5979 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj-art-a58b4c40b32741c2ac7d6eefc0c1b6212025-06-27T05:50:22ZengElsevierPhotoacoustics2213-59792025-08-0144100734Local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detectorDaria Voitovich0Alexey Kurnikov1Anna Orlova2Aleksej Petushkov3Liubov Shimolina4Anastasia Komarova5Marina Shirmanova6Yu-Hang Liu7Daniel Razansky8Pavel Subochev9Institute of Applied Physics, Russian Academy of Sciences, 46 Ulyanov Str., Nizhny Novgorod 603950, Russia; Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod 603005, RussiaInstitute of Applied Physics, Russian Academy of Sciences, 46 Ulyanov Str., Nizhny Novgorod 603950, RussiaInstitute of Applied Physics, Russian Academy of Sciences, 46 Ulyanov Str., Nizhny Novgorod 603950, Russia; Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod 603005, RussiaInstitute of Applied Physics, Russian Academy of Sciences, 46 Ulyanov Str., Nizhny Novgorod 603950, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod 603005, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod 603005, RussiaInstitute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod 603005, RussiaInstitute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, UZH Zurich, Rämistrasse 71, Zurich 8006, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Gloriastrasse 35, Zurich 8092, SwitzerlandInstitute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, UZH Zurich, Rämistrasse 71, Zurich 8006, Switzerland; Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Gloriastrasse 35, Zurich 8092, Switzerland; Corresponding author at: Institute for Biomedical Engineering and Institute of Pharmacology and Toxicology, Faculty of Medicine, UZH Zurich, Rämistrasse 71, Zurich 8006, Switzerland.Institute of Applied Physics, Russian Academy of Sciences, 46 Ulyanov Str., Nizhny Novgorod 603950, Russia; Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, Nizhny Novgorod 603005, Russia; Corresponding author at: Institute of Applied Physics, Russian Academy of Sciences, 46 Ulyanov Str., Nizhny Novgorod 603950, Russia.Optical-resolution optoacoustic (photoacoustic) microscopy is a hybrid imaging modality combining focused optical excitation with ultrasound detection, thus achieving micrometer-scale spatial resolution and high-contrast angiographic imaging. Despite these important advantages, maintaining safe laser fluence levels is essential to prevent tissue damage while ensuring sufficient detection sensitivity. Here, we introduce a model that directly relates the detector’s noise-equivalent pressure (NEP) to the local laser fluence at the imaged blood vessel. The model incorporates acoustic propagation effects from an optoacoustic source to a spherically focused detector with limited aperture and bandwidth, offering a more comprehensive understanding of how fluence and ultrasonic sensitivity are interconnected. The effects of ultrasound generation propagation and detection were accounted for using analytical estimations and numerical simulations, while detector's NEP was experimentally measured with a calibrated hydrophone. The proposed model for evaluating of local laser fluence with a calibrated ultrasound detector was validated through in vitro experiments with superficially located blood layer and numerical Monte Carlo/k-Wave simulations featuring deeper vessels. In vivo experiments employing 532 nm laser excitation and wideband 1–30 MHz ultrasonic detection further demonstrated the model’s capacity for real-time adjustments of laser parameters to ensure tissue safety.http://www.sciencedirect.com/science/article/pii/S2213597925000576Optical-resolution optoacoustic (photoacoustic) microscopySafety limitLaser fluenceNoise equivalent pressureNoise equivalent laser fluenceLocal temperature |
| spellingShingle | Daria Voitovich Alexey Kurnikov Anna Orlova Aleksej Petushkov Liubov Shimolina Anastasia Komarova Marina Shirmanova Yu-Hang Liu Daniel Razansky Pavel Subochev Local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detector Photoacoustics Optical-resolution optoacoustic (photoacoustic) microscopy Safety limit Laser fluence Noise equivalent pressure Noise equivalent laser fluence Local temperature |
| title | Local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detector |
| title_full | Local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detector |
| title_fullStr | Local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detector |
| title_full_unstemmed | Local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detector |
| title_short | Local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detector |
| title_sort | local laser fluence estimation in optical resolution optoacoustic angiography employing calibrated ultrasound detector |
| topic | Optical-resolution optoacoustic (photoacoustic) microscopy Safety limit Laser fluence Noise equivalent pressure Noise equivalent laser fluence Local temperature |
| url | http://www.sciencedirect.com/science/article/pii/S2213597925000576 |
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