Scanned Laser Pico Projection and Stokes-Mueller Matrix Imaging Polarimetry for Detecting Cancer Cells With Different Cytoskeletal Organizations and Metastatic Potencies
The scanned laser pico-projection (SLPP) method and Stokes–Mueller matrix polarimetry technique are used to distinguish cancer cells with different cytoskeletal organizations and metastatic potencies. The speckle contrast of human bone osteosarcoma cancer cells (U2OS) with different cytos...
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IEEE
2018-01-01
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| Series: | IEEE Photonics Journal |
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| Online Access: | https://ieeexplore.ieee.org/document/8556511/ |
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| author | Yu-Jen Chang Yu-Lung Lo Wen-Tai Chiu Chih-Ling Huang Heng-Ai Chang Quoc-Hung Phan |
| author_facet | Yu-Jen Chang Yu-Lung Lo Wen-Tai Chiu Chih-Ling Huang Heng-Ai Chang Quoc-Hung Phan |
| author_sort | Yu-Jen Chang |
| collection | DOAJ |
| description | The scanned laser pico-projection (SLPP) method and Stokes–Mueller matrix polarimetry technique are used to distinguish cancer cells with different cytoskeletal organizations and metastatic potencies. The speckle contrast of human bone osteosarcoma cancer cells (U2OS) with different cytoskeletal drug treatments is measured by the SLPP method. The results show that the contrast is directly related to the organization of the microtubules and actin fibers. The Stokes–Mueller matrix polaimetry technique is employed to measure polarization parameters of the same samples. The measurement results are found to be consistent with those obtained using the SLPP method. Finally, the Stokes–Mueller matrix polaimetry technique is used to extract the polarization parameters of cell-pairs with different metastatic potencies. It is shown that the extracted value of the linear phase retardance (β) is particularly sensitive to the organization of the microtubules and actin fibers. Furthermore, the linear phase retardance (β) and depolarization index (Δ) are both sensitive to the metastatic potency of the cancer cells. In general, both techniques provide a rapid, noninvasive and label-free approach for distinguishing cancer cells with different cytoskeleton organizations and metastatic potentials, and are thus of significant benefit in evaluating the malignancy of cancer cells. |
| format | Article |
| id | doaj-art-a50c2264f6a546569c5fc1de4d9c4a24 |
| institution | Matheson Library |
| issn | 1943-0655 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | IEEE |
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| series | IEEE Photonics Journal |
| spelling | doaj-art-a50c2264f6a546569c5fc1de4d9c4a242025-07-01T23:40:50ZengIEEEIEEE Photonics Journal1943-06552018-01-0110611210.1109/JPHOT.2018.28844488556511Scanned Laser Pico Projection and Stokes-Mueller Matrix Imaging Polarimetry for Detecting Cancer Cells With Different Cytoskeletal Organizations and Metastatic PotenciesYu-Jen Chang0Yu-Lung Lo1https://orcid.org/0000-0002-3217-5038Wen-Tai Chiu2https://orcid.org/0000-0003-0310-0675Chih-Ling Huang3Heng-Ai Chang4Quoc-Hung Phan5Department of Mechanical Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Mechanical Engineering, National Cheng Kung University, Tainan, TaiwanDepartment of Biomedical Engineering, National Cheng Kung University, Tainan, TaiwanCenter for Fundamental Science, Kaohsiung Medical University, Kaohsiung, TaiwanInstitute of Basic Medical Sciences, National Cheng Kung University, Tainan, TaiwanDepartment of Mechanical Engineering, National Cheng Kung University, Tainan, TaiwanThe scanned laser pico-projection (SLPP) method and Stokes–Mueller matrix polarimetry technique are used to distinguish cancer cells with different cytoskeletal organizations and metastatic potencies. The speckle contrast of human bone osteosarcoma cancer cells (U2OS) with different cytoskeletal drug treatments is measured by the SLPP method. The results show that the contrast is directly related to the organization of the microtubules and actin fibers. The Stokes–Mueller matrix polaimetry technique is employed to measure polarization parameters of the same samples. The measurement results are found to be consistent with those obtained using the SLPP method. Finally, the Stokes–Mueller matrix polaimetry technique is used to extract the polarization parameters of cell-pairs with different metastatic potencies. It is shown that the extracted value of the linear phase retardance (β) is particularly sensitive to the organization of the microtubules and actin fibers. Furthermore, the linear phase retardance (β) and depolarization index (Δ) are both sensitive to the metastatic potency of the cancer cells. In general, both techniques provide a rapid, noninvasive and label-free approach for distinguishing cancer cells with different cytoskeleton organizations and metastatic potentials, and are thus of significant benefit in evaluating the malignancy of cancer cells.https://ieeexplore.ieee.org/document/8556511/Cancer cellcytoskeletonscanned laser pico projection (SLPP)stokes-mueller matrix polarimetry |
| spellingShingle | Yu-Jen Chang Yu-Lung Lo Wen-Tai Chiu Chih-Ling Huang Heng-Ai Chang Quoc-Hung Phan Scanned Laser Pico Projection and Stokes-Mueller Matrix Imaging Polarimetry for Detecting Cancer Cells With Different Cytoskeletal Organizations and Metastatic Potencies IEEE Photonics Journal Cancer cell cytoskeleton scanned laser pico projection (SLPP) stokes-mueller matrix polarimetry |
| title | Scanned Laser Pico Projection and Stokes-Mueller Matrix Imaging Polarimetry for Detecting Cancer Cells With Different Cytoskeletal Organizations and Metastatic Potencies |
| title_full | Scanned Laser Pico Projection and Stokes-Mueller Matrix Imaging Polarimetry for Detecting Cancer Cells With Different Cytoskeletal Organizations and Metastatic Potencies |
| title_fullStr | Scanned Laser Pico Projection and Stokes-Mueller Matrix Imaging Polarimetry for Detecting Cancer Cells With Different Cytoskeletal Organizations and Metastatic Potencies |
| title_full_unstemmed | Scanned Laser Pico Projection and Stokes-Mueller Matrix Imaging Polarimetry for Detecting Cancer Cells With Different Cytoskeletal Organizations and Metastatic Potencies |
| title_short | Scanned Laser Pico Projection and Stokes-Mueller Matrix Imaging Polarimetry for Detecting Cancer Cells With Different Cytoskeletal Organizations and Metastatic Potencies |
| title_sort | scanned laser pico projection and stokes mueller matrix imaging polarimetry for detecting cancer cells with different cytoskeletal organizations and metastatic potencies |
| topic | Cancer cell cytoskeleton scanned laser pico projection (SLPP) stokes-mueller matrix polarimetry |
| url | https://ieeexplore.ieee.org/document/8556511/ |
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