Determination of Total Petroleum Hydrocarbon Composition and Screening for Metallotolerant Bacteria from Polluted Effluent Discharge in Kaduna Metropolis, Nigeria
Study’s Excerpt: • Wastewater samples contained high levels of TPHs and heavy metals. • Pseudomonas, Bacillus, and aureus showed strong metal tolerance. • aureus had highest Fe tolerance (0.92 mg/mL); Bacillus for Pb (0.79 mg/mL). • Pseudomonas tolerated Cu best; coli showed Zn resista...
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Umaru Musa Yar'adua University, Katsina, Nigeria
2025-06-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1158 |
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| author | Nomsu Musa Dearest Sambo Glory Masha Mohammed Abdu |
| author_facet | Nomsu Musa Dearest Sambo Glory Masha Mohammed Abdu |
| author_sort | Nomsu Musa |
| collection | DOAJ |
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Study’s Excerpt:
• Wastewater samples contained high levels of TPHs and heavy metals.
• Pseudomonas, Bacillus, and aureus showed strong metal tolerance.
• aureus had highest Fe tolerance (0.92 mg/mL); Bacillus for Pb (0.79 mg/mL).
• Pseudomonas tolerated Cu best; coli showed Zn resistance.
• Isolates are promising for bioremediation in polluted environments.
Full Abstract:
The release of heavy metals into aquatic systems results in various environmental challenges. The combined presence of hydrocarbons and heavy metals within the environment also poses a health risk to humans, plants, and animals. This study aimed to screen for metallotolerant bacteria from a polluted effluent discharge in Kaduna metropolis, Nigeria. Physicochemical assessment, determination of total petroleum content, and heavy metal screening of the wastewater samples were conducted using standard protocols. Metallotolerant bacteria were screened and identified using standard methods. It was revealed from the result that, on the total petroleum hydrocarbons (TPHs), eicosane had the highest detectable similarity with percentage quality (99%), while oxasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15-hexadecamethyl (50%) was the lowest. Lead had the highest concentration of 0.95±0.017 Mg/L, while zinc had the lowest value, 0.057±0.00 mg/L. The bacterial species identified were Escherichia coli, S. aureus, Klebsiella sp., Pseudomonas sp., and Bacillus species, respectively. Furthermore, the tolerance assay results showed that Pseudomonas species had the maximum tolerance for Cu (0.72 mg/mL), Bacillus species for Pb (0.79 mg/mL), Escherichia coli for Zn (0.76 mg/mL), and Staphylococcus aureus for Fe (0.92 mg/mL), respectively. Based on the maximum tolerance exhibited by these bacterial isolates to heavy metals, this study proved they are good candidates, especially isolates of (Staphylococcus aureus, Pseudomonas species, and Bacillus species) for use in bioremediation and bioaugmentation of heavy metals in polluted industrial sites.
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| format | Article |
| id | doaj-art-a4e4fdc4f11c4bf29a6f1a7f0d1111f8 |
| institution | Matheson Library |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-a4e4fdc4f11c4bf29a6f1a7f0d1111f82025-07-29T14:03:24ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222025-06-0110310.47430/ujmr.25103.018Determination of Total Petroleum Hydrocarbon Composition and Screening for Metallotolerant Bacteria from Polluted Effluent Discharge in Kaduna Metropolis, NigeriaNomsu Musa0Dearest Sambo1Glory Masha2Mohammed Abdu3Department of Microbiology, Faculty of Pure and Applied Sciences, Kaduna State University, NigeriaDepartment of Microbiology, Faculty of Pure and Applied Sciences, Kaduna State University, NigeriaDepartment of Microbiology, Faculty of Life Sciences, Bayero University, Kano-NigeriaDepartment of Microbiology, Faculty of Pure and Applied Sciences, Kaduna State University, Nigeria Study’s Excerpt: • Wastewater samples contained high levels of TPHs and heavy metals. • Pseudomonas, Bacillus, and aureus showed strong metal tolerance. • aureus had highest Fe tolerance (0.92 mg/mL); Bacillus for Pb (0.79 mg/mL). • Pseudomonas tolerated Cu best; coli showed Zn resistance. • Isolates are promising for bioremediation in polluted environments. Full Abstract: The release of heavy metals into aquatic systems results in various environmental challenges. The combined presence of hydrocarbons and heavy metals within the environment also poses a health risk to humans, plants, and animals. This study aimed to screen for metallotolerant bacteria from a polluted effluent discharge in Kaduna metropolis, Nigeria. Physicochemical assessment, determination of total petroleum content, and heavy metal screening of the wastewater samples were conducted using standard protocols. Metallotolerant bacteria were screened and identified using standard methods. It was revealed from the result that, on the total petroleum hydrocarbons (TPHs), eicosane had the highest detectable similarity with percentage quality (99%), while oxasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15-hexadecamethyl (50%) was the lowest. Lead had the highest concentration of 0.95±0.017 Mg/L, while zinc had the lowest value, 0.057±0.00 mg/L. The bacterial species identified were Escherichia coli, S. aureus, Klebsiella sp., Pseudomonas sp., and Bacillus species, respectively. Furthermore, the tolerance assay results showed that Pseudomonas species had the maximum tolerance for Cu (0.72 mg/mL), Bacillus species for Pb (0.79 mg/mL), Escherichia coli for Zn (0.76 mg/mL), and Staphylococcus aureus for Fe (0.92 mg/mL), respectively. Based on the maximum tolerance exhibited by these bacterial isolates to heavy metals, this study proved they are good candidates, especially isolates of (Staphylococcus aureus, Pseudomonas species, and Bacillus species) for use in bioremediation and bioaugmentation of heavy metals in polluted industrial sites. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1158Hydrocarbon compositionMetallotolerantBacteriaEffluent dischargeNigeria |
| spellingShingle | Nomsu Musa Dearest Sambo Glory Masha Mohammed Abdu Determination of Total Petroleum Hydrocarbon Composition and Screening for Metallotolerant Bacteria from Polluted Effluent Discharge in Kaduna Metropolis, Nigeria UMYU Journal of Microbiology Research Hydrocarbon composition Metallotolerant Bacteria Effluent discharge Nigeria |
| title | Determination of Total Petroleum Hydrocarbon Composition and Screening for Metallotolerant Bacteria from Polluted Effluent Discharge in Kaduna Metropolis, Nigeria |
| title_full | Determination of Total Petroleum Hydrocarbon Composition and Screening for Metallotolerant Bacteria from Polluted Effluent Discharge in Kaduna Metropolis, Nigeria |
| title_fullStr | Determination of Total Petroleum Hydrocarbon Composition and Screening for Metallotolerant Bacteria from Polluted Effluent Discharge in Kaduna Metropolis, Nigeria |
| title_full_unstemmed | Determination of Total Petroleum Hydrocarbon Composition and Screening for Metallotolerant Bacteria from Polluted Effluent Discharge in Kaduna Metropolis, Nigeria |
| title_short | Determination of Total Petroleum Hydrocarbon Composition and Screening for Metallotolerant Bacteria from Polluted Effluent Discharge in Kaduna Metropolis, Nigeria |
| title_sort | determination of total petroleum hydrocarbon composition and screening for metallotolerant bacteria from polluted effluent discharge in kaduna metropolis nigeria |
| topic | Hydrocarbon composition Metallotolerant Bacteria Effluent discharge Nigeria |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1158 |
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